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Journal of Parasitic Diseases

, Volume 42, Issue 4, pp 604–607 | Cite as

Morphological and molecular identification of Cyclospora species in sheep and goat at Tamil Nadu, India

  • Kanta Basnett
  • K. Nagarajan
  • C. Soundararajan
  • S. Vairamuthu
  • Ganne Venkata Sudhakar Rao
Original Article
  • 14 Downloads

Abstract

A total of 281 fresh fecal samples (65 sheeps and 216 goats) were examined for the presence of Cyclospora species in Tamil Nadu from October 2017 to April 2018. All the faecal samples were examined by direct smear method, saturated sucrose floatation technique and modified Ziehl–Neelsen staining method and it was confirmed—at genus level with PCR technique. Overall prevalence of Cyclospora infection in—small ruminant was 2.14%. The prevalence of Cyclospora species in sheep and goat was 3.08 and 1.85% respectively. Cyclospora oocyst was observed mostly during winter season (Dec–Feb) with 7.14% followed by summer (March and April) and Northeast mansoon (October and November) with 1.67 and 1.66% respectively. The oocyst detected from faecal sample through conventional faecal examination was confirmed by PCR.

Keywords

Cyclospora Sheep Goat Faecal examination PCR Tamil Nadu 

Notes

Author’s contribution

KB Collection and processing of samples and literature collection. KN Assistance in literature collection, typing and preparation of manuscript. CS Assistance in literature collection, typing and preparation of manuscript. SV Assisted in manuscript preparation. VSR Assisted in manuscript preparation.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Indian Society for Parasitology 2018

Authors and Affiliations

  • Kanta Basnett
    • 1
  • K. Nagarajan
    • 1
  • C. Soundararajan
    • 2
  • S. Vairamuthu
    • 3
  • Ganne Venkata Sudhakar Rao
    • 1
  1. 1.Department of Veterinary Pathology, Madras Veterinary CollegeTamil Nadu Veterinary and Animal Sciences UniversityChennaiIndia
  2. 2.Department of Veterinary Parasitology, Madras Veterinary CollegeTamil Nadu Veterinary and Animal Sciences UniversityChennaiIndia
  3. 3.Centralised Clinical Laboratory, Madras Veterinary CollegeTamil Nadu Veterinary and Animal Sciences UniversityChennaiIndia

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