Morphological and molecular identification of Cyclospora species in sheep and goat at Tamil Nadu, India
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A total of 281 fresh fecal samples (65 sheeps and 216 goats) were examined for the presence of Cyclospora species in Tamil Nadu from October 2017 to April 2018. All the faecal samples were examined by direct smear method, saturated sucrose floatation technique and modified Ziehl–Neelsen staining method and it was confirmed—at genus level with PCR technique. Overall prevalence of Cyclospora infection in—small ruminant was 2.14%. The prevalence of Cyclospora species in sheep and goat was 3.08 and 1.85% respectively. Cyclospora oocyst was observed mostly during winter season (Dec–Feb) with 7.14% followed by summer (March and April) and Northeast mansoon (October and November) with 1.67 and 1.66% respectively. The oocyst detected from faecal sample through conventional faecal examination was confirmed by PCR.
KeywordsCyclospora Sheep Goat Faecal examination PCR Tamil Nadu
KB Collection and processing of samples and literature collection. KN Assistance in literature collection, typing and preparation of manuscript. CS Assistance in literature collection, typing and preparation of manuscript. SV Assisted in manuscript preparation. VSR Assisted in manuscript preparation.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
- Orlandi PA, Frazar C, Carter L, Chu DT (2004) Detection of Cyclospora and Cryptosporidium from fresh produce: isolation and identification by polymerase chain reaction (PCR) and microscopic analysis. Bacteriol Anal Manual, Chapter 19a. U.S. Food and Drug Administration. https://www.fda.gov/food/foodscienceresearch/laboratorymethods/ucm073638.htm. Accessed 12 Sept 2018