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Journal of Parasitic Diseases

, Volume 40, Issue 3, pp 1038–1043 | Cite as

In vitro production of Toxocara canis excretory-secretory (TES) antigen

  • Divyamol Thomas
  • N. Jeyathilakan
  • S. Abdul Basith
  • T. M. A. Senthilkumar
Original Article

Abstract

Toxocara canis is a widespread gastrointestinal nematode parasite of dogs and cause Toxocara larva migrans, an important zoonotic disease in humans on ingestion of infective eggs. Toxocarosis is one of the few human parasitic diseases whose serodiagnosis uses a standardized antigen, T. canis excretory secretory antigen (TES). The present study describes collection of T. canis adult worm, collection and embryonation of T. canis eggs, hatching and separation of T. canis larvae, in vitro maintenance of T. canis second stage larvae for production of TES, concentration of culture fluid TES and yield of TES in correlation with various methods cited in literature.

Keywords

Toxocara canis In vitro cultivation Excretory-secretory antigen 

Notes

Acknowledgments

The authors wishes to thank Dr. S.A. ASOKAN, Dean, Madras Veterinary College, Chennai, India for the facilities provided. The work was supported by Indian Council of Agricultural Research, New Delhi, India with financial assistance in the form of Junior Research Fellowship.

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Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • Divyamol Thomas
    • 1
  • N. Jeyathilakan
    • 2
  • S. Abdul Basith
    • 1
  • T. M. A. Senthilkumar
    • 3
  1. 1.Department of Veterinary ParasitologyMadras Veterinary College (TANUVAS)ChennaiIndia
  2. 2.Department of Veterinary ParasitologyVeterinary College and Research Institute (TANUVAS)OrathanaduIndia
  3. 3.Department of Animal BiotechnologyMadras Veterinary College (TANUVAS)ChennaiIndia

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