Journal of Parasitic Diseases

, Volume 40, Issue 3, pp 981–985 | Cite as

IL-18 gene polymorphism in patients with visceral leishmaniasis in East Azarbaijan, Iran

  • Ehsan Ahmadpour
  • Ahad BazmaniEmail author
  • Mohamad Hasan Kohansal
  • Abdolhasan Kazemi
  • Zohre Babaloo
Original Article


Visceral leishmaniasis (VL) is a parasitic disease caused by Leishmania species. According to the important role of cellular immunity against VL, this study was directed to determine the frequency of −607A/C and −137G/C genotypes on promoter region of interleukin-18 gene. The study groups included 91 patients with confirmed history of VL, 106 healthy seronegative, and 79 healthy seropositive individuals. All three groups were analyzed by amplification refractory mutation system polymerase chain reaction (ARMS-PCR). The highest rate of −607/A, and −607/C alleles was observed in seronegative individuals (66/67 %) and in the patients (72/83 %). Allele frequency of −607/C is more than −607/A allele in all groups. In position of −137, frequency of −137/G allele in all groups was more than −137/C. Statistical analysis of distribution of genotypes, did not reveal any significant difference among groups. On the basis of the results, there was no significant association between VL and polymorphism of IL-18 promoter. The results of this study showed that IL-18 gene promoter polymorphisms at positions −607 and −137 are not associated with VL in East Azerbaijan, Iran.


Interleukin-18 Visceral leishmaniasis ARMS-PCR East Azerbaijan Iran 



This project was supported by a grant from the Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences.

Conflict of interests

The authors declare that there is no conflict of interests.


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Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • Ehsan Ahmadpour
    • 1
    • 2
  • Ahad Bazmani
    • 2
    Email author
  • Mohamad Hasan Kohansal
    • 3
  • Abdolhasan Kazemi
    • 4
  • Zohre Babaloo
    • 5
  1. 1.Toxoplasmosis Research CenterMazandaran University of Medical SciencesSariIran
  2. 2.Infectious and Tropical Diseases Research CenterTabriz University of Medical SciencesTabrizIran
  3. 3.Department of Medical Parasitology and Mycology, School of MedicineZanjan University of Medical SciencesZanjanIran
  4. 4.Drug Applied Research Center, Department of Medical Parasitology and MycologyTabriz University of Medical SciencesTabrizIran
  5. 5.Immunology Research CenterTabriz University of Medical SciencesTabrizIran

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