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Journal of Parasitic Diseases

, Volume 40, Issue 3, pp 910–913 | Cite as

2D-PAGE analysis of the soluble proteins of the tropical liver fluke, Fasciola gigantica and biliary amphistome, Gigantocotyle explanatum, concurrently infecting Bubalus bubalis

  • Yasir A. Khan
  • Mohd Abdul Hannan Khan
  • S. M. A. Abidi
Original Article

Abstract

The digenetic trematodes, Fasciola gigantica and Gigantocotyle explanatum, belonging to the family Fasciolidae and Paramphistomidae respectively, have been often found to concurrently infect the liver of Indian water buffalo Bubalus bubalis, causing serious pathological damage to the vital organ, incurring huge economic losses. In the present study the soluble gene products of both F. gigantica and G. explanatum were analyzed by 2 dimensional polyacrylamide gel electrophoresis. The soluble proteomic profile revealed considerable similarity as well as differences in the size, distribution pattern, total number, the isoelectric point (pI) and molecular weight (Mr) of the resolved polypeptide spots. The maximum number of polypeptide spots with a molecular weight range of >10 to 160 kDa were recorded with a pI range of 7–9 followed by pI range of 5–7, 9–10 and 3–5 in both the parasites. However, considerable variation was recorded in the Mr of the polypeptides belonging to each pI range. The genetic heterogeneity could be an obvious contributing factor for such differences but some polypeptides appeared to be conserved in the two species. The molecular similarities and the habitat preference by these worms may be a consequence of microenvironmental cues that guide these flukes to reach their habitat through different routes and establish a successful host-parasite relationship.

Keywords

Digenetic trematodes 2D PAGE Concurrent infection Bubalus bubalis 

Notes

Acknowledgments

Authors are grateful to the Chairman, Department of Zoology, Aligarh Muslim University for providing the necessary facilities. Thanks are extended to University Grants Commission, New Delhi for financial assistance to YAK. Financial support under the BBSRC UK CIDLID project to SMAA and fellowship to MAHK is also gratefully acknowledged.

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Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • Yasir A. Khan
    • 1
  • Mohd Abdul Hannan Khan
    • 1
  • S. M. A. Abidi
    • 1
  1. 1.Section of Parasitology, Department of ZoologyAligarh Muslim UniversityAligarhIndia

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