Laboratory utility of coproscopy, copro immunoassays and copro nPCR assay targeting Hsp90 gene for detection of Cryptosporidium in children, Cairo, Egypt
- 116 Downloads
Cryptosporidium is a significant cause of diarrhea worldwide especially in children. Infection may end fatally in immunocompromised patients. Multi-attribute analysis was used to determine the lab utility of 4 diagnostics; coproscopy of AF stained fecal smear, fecal immunoassays by ICT and ELISA and copro-nPCR assay targeting Hsp90 gene, for detection of Cryptosporidium in stool of 250 Egyptian children (150 diarrheic and 100 non-diarrhaeic children). Also, to determine Cryptosporidium molecular prevalence. Cryptosporidium was an important enteric pathogen among both diarrheic and non-diarrheic study children with a clearly high prevalence of 16.4 % (n = 41). Conventional methods had perfect specificity (100 %) but couldn`t be used as a consistent single detection method due to their lowered sensitivities. Multi-attribute analysis ranked nPCR the highest test for lab use. Being the test with the best diagnostic yield, nPCR is a reliable diagnostic test and is going to replace conventional methods for reliable detection of Cryptosporidium.
KeywordsCryptosporidium Hsp90 Copro-DNA Nested PCR Multi-attribute Fecal immunoassay ELISA ICT AF stain
Conflict of interest
The authors declare that they have no Conflict of interests.
- AL-Hindi AI, EL Manama AA, Elnabris KJ (2007) Cryptosporidiosis among children attending Al-Nasser Pediatric Hospital, Gaza, Palestine. Turk J Med Sci 37:367–372Google Scholar
- El-Hamshary EM, El-Sayed HF, Hussein EM, Rayan HZ, Rasha H, Soliman RH (2008) Comparison of polymerase chain reaction, immunochromatographic assay and staining techniques in diagnosis of cryptosporidiosis. PUJ 1:77–86Google Scholar
- El-Settawy MA, Fathy GM (2012) Evaluation and comparison of PCR, coproantigen ELISA and microscopy for diagnosis of Cryptosporidium in human diarrheic specimens. J Am Sci 8:1385Google Scholar
- Garcia LS (2007) Diagnostic Medical Parasitology, 5th edn. ASM Press, Washington D. CGoogle Scholar
- Khurana S, Sharma P, Sharma A, Malla N (2012) Evaluation of Ziehl-Neelsen staining, auramine phenol staining, antigen detection enzyme linked immunosorbent assay and polymerase chain reaction, for the diagnosis of intestinal cryptosporidiosis. Trop Parasitol 2:20–23CrossRefPubMedPubMedCentralGoogle Scholar
- Mac Pherson DW, Mc Queen R (1993) Cryptosporidiosis: multi-attribute evaluation of six diagnostic methods. J Clin Microbiol 31:198–202Google Scholar
- Salyer S, Gillespie T, Rwego I, Chaman C, Goldberg T (2012) Epidemiology and molecular relationships of Cryptosporidium spp. in people, primates, and livestock from Western Uganda. PLoS 6:1–6Google Scholar
- Smith HV (2008) Diagnostics. In: Fayer R, Xiao L (eds) Cryptosporidiosis of man and animals. CRC Press and IWA Publishing, Boca Raton, pp 173–208Google Scholar
- Tahira F, Khan HM, Shukla I, Shujatullah F, Malik MA, Shahid M (2012) Prevalence of Cryptosporidium in children with diarrhoea in north Indian tertiary care hospital. J Community Med Health Edu 2:1–3Google Scholar