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Journal of Parasitic Diseases

, Volume 40, Issue 3, pp 779–784 | Cite as

Histopathological and bacterial study of Persian sturgeon fry, Acipenser persicus (Borodin, 1897) exposed to copper sulfate and potassium permanganate

  • Batol Moshtaghi
  • Hossein Khara
  • Zabiyollah Pazhan
  • Alireza Shenavar
Original Article
  • 81 Downloads

Abstract

Persian sturgeon frys were exposed to different concentrations of copper sulfate and potassium permanganate in order to the evaluation of their impacts on bacterial load of skin, gill and surrounding water and also the histopathological alternations of gill tissue. For this purpose, the sublethal doses were determined after a pre-test and then the experiment was done in 4 (for copper sulfate: 0.07, 0.14, 026 and 0.5 mg/l) and 5 (for potassium permanganate: 0.07, 0.14, 026, 0.5 and 1 mg/l) treatments with three replicates inside the glass aquaria. Also, one group without disinfecting drug was considered as control for each experiment. The microbial and histopathological investigations were done after 96 h exposure. According to our results, a range of histopathological alternations were observed in gills tissue including mucus coagulation and secretion, hyperplasia, lamellar necrosis, hyperplasia, lamellar adhesion, haemorrhage, thickening of secondary lamellae, hypertrophy of supporter cartilage, clubbing of gill lamellae and sliming of primary lamellae. The severity of these alternations increased with increasing of the doses of the copper sulfate and potassium permanganate. The bacterial load (CFU/g) of gill, skin and surrounding water was lower in 0.07 mg/l copper sulfate treatment and 1 mg/l potassium permanganate treatment (P < 0.05) than in other treatments. In conclusion, our results showed that the certain doses of the copper sulfate and potassium permanganate have disinfecting effects on bacterial load of gill, skin and surrounding water, although this is along with some histopathological alternations. Also, it seems that the copper sulfate has higher disinfecting power than potassium permanganate.

Keywords

Copper sulfate Potassium permanganate Histopathology Gill Persian sturgeon 

Notes

Acknowledgments

The authors would like to acknowledge head and staff of International Sturgeon Research Institute for technical assistance throughout the course of the study and providing of fish. The authors also acknowledge Mr. Ali Hallajianand, Mrs. Roghayeh Fathollahi for their helpful comments and assistances during the course of the experiment.

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Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • Batol Moshtaghi
    • 1
  • Hossein Khara
    • 2
  • Zabiyollah Pazhan
    • 3
  • Alireza Shenavar
    • 3
  1. 1.Young Researchers Club, Lahijan BranchIslamic Azad UniversityLahijanIran
  2. 2.Department of Fishery, College of Natural Resources, Lahijan BranchIslamic Azad UniversityLahijanIran
  3. 3.The International Research Institute of SturgeonRashtIran

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