pp 1–10 | Cite as

Effects of Silicon on the Growth, Photosynthesis and Chloroplast Ultrastructure of Oryza sativa L. Seedlings under Acid Rain Stress

  • Shuming Ju
  • Liping WangEmail author
  • Jiayi Chen
Original Paper


Silicon (Si) is a beneficial element for plants and can increase plant resistance. In the present work, a hydroponic experiment was carried out to study the effects of Si on the growth and photosynthesis of rice (Oryza sativa L.) seedlings under simulated acid rain (SAR) stress. The growth, photosynthesis and chloroplast ultrastructure of rice seedlings treated with combined or single weak SAR (pH 4.0) and/or Si (1, 2 or 4 mM) were improved. Spraying with moderate or severe SAR (pH 3.0 or 2.0) significantly inhibited the growth and photosynthesis and severely damaged the chloroplast ultrastructure of rice seedlings. The incorporation of exogenous Si increased the growth and photosynthesis and improved the chloroplast ultrastructure of rice seedlings treated with moderate or severe SAR (pH 3.0 or 2.0). The 2.0 mM Si treatment had more significant promoting or alleviating effects than the 1 and 4 mM Si treatments. The stomatal conductance (Gs), chlorophyll content, maximum quantum efficiency of PSII photochemistry (Fv/Fm), actual photochemical quantum efficiency of PSII photochemistry (Y) and chloroplast ultrastructure were improved with the addition of Si to the SAR treatment, which indicated that the positive effect of Si on photosynthesis was partly associated with stomatal and non-stomatal factors. Thus, Si fertilization improves rice resistance to acid rain.


Silicon Acid rain Oryza sativa L. Growth Photosynthesis 


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The National Spark Plan Project (Nos. S2013C100537) and the Construction Projects Jiangsu Laboratory of Pollution Control and Resource Reuse.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.


  1. 1.
    Abbasi T, Poornima P, Kannadasan T, Abbasi SA (2013) Acid rain: past, present, and future. Int J Environ Eng 97:229–272CrossRefGoogle Scholar
  2. 2.
    Ju SM, Wang LP, Yin NN, Li D, Wang YK, Zhang CY (2017a) Silicon alleviates simulated acid rain stress of Oryza sativa L. seedlings by adjusting physiology activity and mineral nutrients. Protoplasma 254:2071–2081. CrossRefGoogle Scholar
  3. 3.
    Wang M, Tang Y, Anderson CWN, Jeyakumar P, Yang J (2018) Effect of simulated acid rain on fluorine mobility and the bacterial community of phosphogypsum. Environ Sci Pollut R 25:15336–15348CrossRefGoogle Scholar
  4. 4.
    Singh A, Agrawal M (2008) Acid rain and its ecological consequences. J Environ Biol 29:15–24Google Scholar
  5. 5.
    Hu HQ, Wang LH, Zhou Q, Huang XH (2016b) Combined effects of simulated acid rain and lanthanum chloride on chloroplast structure and functional elements in rice. Environ Sci Pollut Res 23:8902–8916CrossRefGoogle Scholar
  6. 6.
    Wang L, Wang W, Zhou Q, Huang X (2014b) Combined effects of lanthanum (III) chloride and acid rain on photosynthetic parameters in rice. Chemosphere 112:355–361CrossRefGoogle Scholar
  7. 7.
    Wang YW, Jiang XH, Li K, Wu M, Zhang RF, Zhang L, Chen GX (2014a) Photosynthetic responses of Oryza sativa L. seedlings to cadmium stress: physiological, biochemical and ultrastructural analyses. Biometals 27:389–401CrossRefGoogle Scholar
  8. 8.
    Sun Z, Wang L, Zhou Q, Huang X (2013) Effects and mechanisms of the combined pollution of lanthanum and acid rain on the root phenotype of soybean seedlings. Chemosphere 93:344–352CrossRefGoogle Scholar
  9. 9.
    Macaulay BM, Enahoro GE (2015) Effects of simulated acid rain on the morphology, phenology and dry biomass of a local variety of maize (Suwan-1) in southwestern Nigeria. Environ Monit Assess 187:622CrossRefGoogle Scholar
  10. 10.
    Debnath B, Irshad M, Mitra S, Li M, Hm R, Liu S et al (2018) Acid rain deposition modulates photosynthesis, enzymatic and non-enzymatic antioxidant activities in tomato. Int J Environ Res 2018(12):203–214CrossRefGoogle Scholar
  11. 11.
    Wang T, Yang W, Xie Y, Shi D, Ma Y, Sun X (2017) Effects of exogenous nitric oxide on the photosynthetic characteristics of bamboo (Indocalamus barbatus McClure) seedlings under acid rain stress. Plant Growth Regul 82:69–78CrossRefGoogle Scholar
  12. 12.
    Liu MH, Yi LT, Yu SQ, Yu F, Yin XM (2015) Chlorophyll fluorescence characteristics and the growth response of Elaeocarpusglabripetalusto simulated acid rain. Photosynthetica 53:23–28CrossRefGoogle Scholar
  13. 13.
    Bernacchi CJ, Bagley JE, Serbin SP, Ruiz-Vera UM, Rosenthal DM, Vanloocke A (2013) Modelling C3 photosynthesis from the chloroplast to the ecosystem. Plant Cell Environ 36:1641–1657CrossRefGoogle Scholar
  14. 14.
    Li P, Song A, Li Z, Fan F, Liang Y (2015) Silicon ameliorates manganese toxicity by regulating both physiological processes and expression of genes associated with photosynthesis in rice (Oryza sativa L.). Plant Soil 397:289–301CrossRefGoogle Scholar
  15. 15.
    Hodson MJ, White PJ, Mead A, Broadley MR (2005) Phylogenetic variation in the silicon composition of plants. Ann Bot 96:1027–1046CrossRefGoogle Scholar
  16. 16.
    Katz O (2015) Silica phytoliths in angiosperms: phylogeny and early evolutionary history. New Phytol 208:642–646CrossRefGoogle Scholar
  17. 17.
    Bogdan K, Schenk MK (2008) Arsenic in rice (Oryza sativa L.) related to dynamics of arsenic and silicic acid in paddy soils. Environ Sci Technol 42:7885–7890CrossRefGoogle Scholar
  18. 18.
    Liang YC, Hua HX, Zhu YG, Zhang J, Cheng CM, Romheld V (2006) Importance of plant species and external silicon concentration to active silicon uptake and transport. New Phytol 172:63–72CrossRefGoogle Scholar
  19. 19.
    Cooke J, Leishman MR (2016) Consistent alleviation of abiotic stress with silicon addition: a meta-analysis. Funct Ecol 30:1340–1357CrossRefGoogle Scholar
  20. 20.
    Detmann KC, Araújo WL, Martins SCV, Sanglard LMVP, Reis JV, Detmann E, Rodrigues FÁ, Nunes-Nesi A, Fernie AR, DaMatta FM (2012) Silicon nutrition increases grain yield, which, in turn, exerts a feed-forward stimulation of photosynthetic rates via enhanced mesophyll conductance and alters primary metabolism in rice. New Phytol 196:752–762CrossRefGoogle Scholar
  21. 21.
    Guntzer F, Catherine KFG, Meunier JD (2012) Benefits of plant silicon for crops: a review. Agron Sustain Dev 32:201–213CrossRefGoogle Scholar
  22. 22.
    Mateos-Naranjo E, Andrades-Moreno L, Davy AJ (2013) Silicon alleviates deleterious effects of high salinity on the halophytic grass Spartina densiflora. Plant Physi Bioch 63:115–121CrossRefGoogle Scholar
  23. 23.
    Sahebi M, Hanafi MM, Akmar ASN, Rafii MY, Azizi P, Tengoua FF et al (2015) Importance of silicon and mechanisms of biosilica formation in plants. BioMed Res Int 2015:1–16. CrossRefGoogle Scholar
  24. 24.
    Song A, Li P, Fan F, Li Z, Liang Y (2014) The effect of silicon on photosynthesis and expression of its relevant genes in rice (Oryza sativa L.) under high-zinc stress. PLoS One 9(11):e113782. CrossRefGoogle Scholar
  25. 25.
    Larssen T, Lydersen E, Tang D, He Y, Gao J, Liu H, Duan L, Seip HM, Vogt RD, Mulder J, Shao M, Wang Y, Shang H, Zhang X, Solberg S, Aas W, Okland T, Eilertsen O, Angell V, Li Q, Zhao D, Xiang R, Xiao J, Luo J (2006) Acid rain in China. Environ Sci Technol 40:418–425CrossRefGoogle Scholar
  26. 26.
    Wailes EJ, Cramer GL, Chavez EC, Hansen JM (2010) Arkansas global rice model: international baseline projections for 1997–2010. Arkansas Agricultural Experiment Station, Arkansas, pp 1–46Google Scholar
  27. 27.
    Yamaji N, Ma JF (2011) Further characterization of a rice silicon efflux transporter, Lsi2. Soil Sci Plant Nutr 57:259–264CrossRefGoogle Scholar
  28. 28.
    Ju SM, Yin NN, Wang LP, Zhang CY, Wang YK (2017b) Effects of silicon on Oryza sativa L. seedling roots under simulated acid rain stress. PLoS One 12(3):1–19CrossRefGoogle Scholar
  29. 29.
    Ju SM, Wang LP, Zhang CY, Yin TC, Shao SL (2017c) Alleviatory effects of silicon on the foliar micromorphology and anatomy of rice (Oryza sativa L.) seedlings under simulated acid rain. PLoS One 12(10):e0187021. CrossRefGoogle Scholar
  30. 30.
    Yoshida S, Forno DA, Cock J (1976) Laboratory manual for physiological studies of rice. Int Rice Res InstGoogle Scholar
  31. 31.
    Liang J (2008) A study on effeets of acid rain on soil, yield and quality forming of crops in Nanjing, dissertation. Nanjing University of InformationGoogle Scholar
  32. 32.
    Wang C, Guo P, Han G, Feng X, Zhang P, Tian X (2010) Effect of simulated acid rain on the litter decomposition of Quercusacutissima and Pinusmassoniana in forest soil microcosms and the relationship with soil enzyme activities. Sci Total Environ 408:2706–2713CrossRefGoogle Scholar
  33. 33.
    Jin X, Wen X, Xie X, Yu J, Lu ZW (2015) Analysis of historical change of acid rain pollution trend of Nanjing (in Chinese). The Administration and Technique of Environmental Monitoring 27:65–68Google Scholar
  34. 34.
    Luo X, Li J, Zhang P, Zhu ZZ, Li Y (2013) Advances in research on the chemical composition of precipitation and its sources in China. Earth Environ 41:566–575Google Scholar
  35. 35.
    Wen K, Liang C, Wang L, Hu G, Zhou Q (2011) Combined effects of lanthanumion and acid rain on growth, photosynthesis and chloroplast ultrastructure in soybean seedlings. Chemosphere 84:601–608CrossRefGoogle Scholar
  36. 36.
    Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148:350–382CrossRefGoogle Scholar
  37. 37.
    Vicherová E, Hájek M, Hájek T (2015) Calcium intolerance of fen mosses: physiological evidence, effects of nutrient availability and successional drivers. Perspect Plant Ecol Evol Syst 17:347–359. CrossRefGoogle Scholar
  38. 38.
    Imran MA, Hussain S, Hussain M, Ch MN, Meo AA (2014) Effect of simulated acid rain (SAR) on some morphochemical aspects of mash (Vigna mungo L.). Pak J Bot 46:245–250Google Scholar
  39. 39.
    Ramlall C, Varghese B, Ramdhani S, Pammenter NW, Bhatt A, Berjak P, Sershen (2015) Effects of simulated acid rain on germination, seedling growth and oxidative metabolism of recalcitrant-seeded Trichiliadregeana grown in its natural seed bank. Physiol Plant 153:149–160CrossRefGoogle Scholar
  40. 40.
    Sun J, H H, Li Y, Wang L, Zhou Q, Huang X (2016) Effects and mechanism of acid rain on plant chloroplast ATP synthase. Environ Sci Pollut Res 23:18296–18306CrossRefGoogle Scholar
  41. 41.
    Abe SS, Yamasaki Y, Wakatsuki T (2016) Assessing silicon availability in soils of rice-growing lowlands and neighboring uplands in Benin and Nigeria. Rice Sci 23(4):196–202CrossRefGoogle Scholar
  42. 42.
    Fan XY, Lin WP, Liu R, Jiang NH, Cai KZ (2018) Physiological response and phenolic metabolism in tomato (Solanum lycopersicum) mediated by silicon under Ralstonia solanacearum infection. J Integr Agr 17(10):2160–2171CrossRefGoogle Scholar
  43. 43.
    Zhang Y, Shi Y, Gong HJ, Zhao Hi LIHL, Hu YH, Wang YC (2018) Beneficial effects of silicon on photosynthesis of tomato seedlings under water stress. J Integr Agr 17(10):2151–2159CrossRefGoogle Scholar
  44. 44.
    Velikova V, Tsonev T, Yordanov I (1999) Light and CO2 responses of photosynthesis and chlorophyll fluorescence characteristics in bean plants after simulated acid rain. Physiol Plant 107:77–83CrossRefGoogle Scholar
  45. 45.
    Liu J, Zhou G, Yang C, Ou Z, Peng C (2007) Responses of chlorophyll fluorescence and xanthophyll cycle in leaves of Schimasuperba Gardn. & champ. And Pinus massoniana lamb. To simulated acid rain at Dinghushan biosphere reserve, China. Acta Physiol Plant 29:33–38CrossRefGoogle Scholar
  46. 46.
    Hu HQ, Wang LH, Li YL, Sun JW, Zhou Q, Huang XH (2016a) Insight into mechanism of lanthanum (III) induced damage to plant photosynthesis. Ecotoxicol Environ Saf 127:43–50CrossRefGoogle Scholar
  47. 47.
    L’Huillier L, d’Auza J, Durand M, Michaud-Ferrière N (1996) Nickel effects on two maize (Zea mays) cultivars: growth, structure, Ni concentration, and localization. Can J Bot 74:1547–1554CrossRefGoogle Scholar
  48. 48.
    Rajpoot R, Rani A, Srivastava RK, Pandey P, Dubey RS (2016) Terminalia arjuna bark extract alleviates nickel toxicity by suppressing its uptake and modulating antioxidativedefence in rice seedlings. Protoplasma 253:1449–1462CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.School of Environment and Spatial InformaticsChina University of Mining & TechnologyXuzhouChina
  2. 2.Xuzhou Institute of TechnologyXuzhouChina

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