Bacteriocin of Pediococcus acidilactici HW01 Inhibits Biofilm Formation and Virulence Factor Production by Pseudomonas aeruginosa
Pseudomonas aeruginosa is a potential source of food contamination that leads to food spoilage and infections as a result of the generation of biofilm and virulence factors. In the present study, we demonstrate that bacteriocin produced by Pediococcus acidilactici HW01 (HW01 bacteriocin) effectively inhibited the biofilm formation of Ps. aeruginosa (66.41, 45.77, and 21.73% of biofilm formation at 0.5, 1, and 2 mg/mL of HW01 bacteriocin, respectively) as well as the production of virulence factors. By means of a microtiter plate method and scanning electron microscopy, HW01 bacteriocin inhibited biofilm formation by Ps. aeruginosa in a dose-dependent manner. Although the viability of biofilm cells of Ps. aeruginosa was reduced in the presence of HW01 bacteriocin, the viability of planktonic cells of Ps. aeruginosa was not affected by HW01 bacteriocin (2.0 × 109 CFU/mL vs. 2.4 × 109 CFU/mL in the absence and the presence of HW01 bacteriocin, respectively). Additionally, HW01 bacteriocin decreased the twitching motility of Ps. aeruginosa as well as the production of virulence factors, such as pyocyanin, protease, and rhamnolipid. Furthermore, HW01 bacteriocin significantly inhibited Ps. aeruginosa biofilm formation on the surface of stainless steel (57% reduction at 24 h and 83% reduction at 72 h). These results indicate that HW01 bacteriocin is an effective antagonist of Ps. aeruginosa as a result of its ability to inhibit biofilm formation and the production of virulence factors.
KeywordsPediococcus acidilactici Bacteriocin Pseudomonas aeruginosa Biofilm Virulence factor
The authors are grateful to Prof. Wang June Kim, Department of Food Science and Biotechnology, Dongguk University, Goyang, Korea, for providing Ped. acidilactici HW01 used in this study.
This work was supported by a grant from the National Research Foundation of Korea, which is funded by the Korean government (NRF-2017R1D1A1B03028730).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 26.Soccol CR, Vandenberghe LPD, Spier MR, Medeiros ABP, Yamaguishi CT, Lindner JD, Pandey A, Thomaz-Soccol V (2010) The potential of probiotics: a review. Food Technol Biotech 48:413–434Google Scholar
- 41.de la Fuente-Nunez C, Korolik V, Bains M, Nguyen U, Breidenstein EB, Horsman S, Lewenza S, Burrows L, Hancock RE (2012) Inhibition of bacterial biofilm formation and swarming motility by a small synthetic cationic peptide. Antimicrob Agents Chemother 56:2696–2704PubMedPubMedCentralCrossRefGoogle Scholar
- 50.Das T, Kutty SK, Tavallaie R, Ibugo AI, Panchompoo J, Sehar S, Aldous L, Yeung AW, Thomas SR, Kumar N, Gooding JJ, Manefield M (2015) Phenazine virulence factor binding to extracellular DNA is important for Pseudomonas aeruginosa biofilm formation. Sci Rep 5:8398PubMedPubMedCentralCrossRefGoogle Scholar