Variability in Probiotic Formulations Revealed by Proteomics and Physico-chemistry Approach in Relation to the Gut Permeability
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Variability in the efficacy, safety, and quality of probiotic formulations depends on many factors, including process conditions used by manufacturers. Developing reliable analytical tools is therefore essential to quickly monitor manufacturing differences in probiotic samples for their quality assessment. Here, multi-strain probiotics from two production sites and countries were investigated by proteomics and physico-chemistry approaches in relation to the protective effect on gut barrier. Proteomic analyses showed differences in protein abundances, identities, and origins of two series of VSL#3 samples from different sites. Even though both formulations were qualitatively similar in thermal and colloidal profiles, significant differences were quantitatively observed in terms of maximum decomposition temperature Tmax (p < 0.05) and phase transition temperature Tm (p < 0.01). Such variability in physical and biochemical features impacts on probiotic functionalities and translates into a differential modulation of gut permeability in mice. Physico-chemical scans provide coherent data with proteomics and represent a new tool for time and cost effective quality control of probiotic-based products.
KeywordsThermal decomposition Transition phase Colloidal properties Intestinal permeability Multistrain probiotics
We acknowledge Mrs. Lynn Doran for DSC and TGA analyses at the Laboratory of Food Sciences and Formulation in Gembloux Agro-Bio Tech (BE). The authors wish to thank Prof. Claudio De Simone, the inventor of the De Simone Formulation, US patent 5,716,615.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have received no financial support for this study. The proteomic analysis by Creative Proteomic was commissioned by Prof. C. de Simone.
This study was conducted in agreement with the Italian law, and the protocol was approved by the Ethics Committee and by a National Committee of Ministry of Health (permission no. 1126/2016-PR).
- 2.Razafindralambo H (2018a) Trends in probiotic applications. Studium Press LLC, HoustonGoogle Scholar
- 3.Soccol CR, Vandenberghe LP d S, Spier MR, Medeiros ABP, Yamaguishi CT, Lindner JDD, Pandey A, Thomaz-Soccol V (2010) The potential of probiotics: a review. Food Technol Biotechnol 48:413–434Google Scholar
- 9.Song D, Ibrahim S, Hayek S (2012) Recent application of probiotics in food and agricultural science. Probiotics 10:1–34Google Scholar
- 10.Guarner F, Khan AG, Garisch J, Eliakim R, Gangl A, Thomson A, Krabshuis J, Lemair T, Kaufmann P, de Paula JA (2012) World gastroenterology organisation global guidelines: probiotics and prebiotics october 2011. J Clin Gastroenterol 46:468–481. https://doi.org/10.1097/MCG.0b013e3182549092 CrossRefPubMedGoogle Scholar
- 11.Lee YK, Salminen S (2009) Handbook of probiotics and prebiotics. John Wiley & SonsGoogle Scholar
- 16.Razafindralambo H (2018b) Advances in physical chemistry tools for probiotic characterization. In: Razafindralambo H (ed) Trends in probiotic applications. Studium Press LLC, Houston, pp 50–81Google Scholar
- 19.Biagioli M, Laghi L, Carino A, Cipriani S, Distrutti E, Marchianò S, Parolin C, Scarpelli P, Vitali B, Fiorucci S (2017) Metabolic variability of a multispecies probiotic preparation impacts on the anti-inflammatory activity. Front Pharmacol 8:505. https://doi.org/10.3389/fphar.2017.00505 CrossRefPubMedPubMedCentralGoogle Scholar
- 20.Cinque B, La Torre C, Lombardi F, Palumbo P, Van der Rest M, Cifone MG (2016) Production conditions affect the in vitro anti-tumoral effects of a high concentration multi-strain probiotic preparation. PLoS One 11:e0163216. https://doi.org/10.1371/journal.pone.0163216 CrossRefPubMedPubMedCentralGoogle Scholar
- 22.Aires J, Anglade P, Baraige F, Zagorec M, Champomier-Vergès MC, Butel MJ (2010) Proteomic comparison of the cytosolic proteins of three Bifidobacterium longum human isolates and Bifidobacterium longum NCC2705. BMC Microbiol 10:29. https://doi.org/10.1186/1471-2180-10-29 CrossRefPubMedPubMedCentralGoogle Scholar
- 25.Cifone MG, Cinque B, La Torre C, Lombardi F, Palumbo P, van den Rest ME, Vuotto C, Donelli G. (2017) Complexities and pitfalls in the production of multispecies probiotics: the paradigmatic case of VSL#3 formulation and visbiome. In: Floch M H, Ringel T and Walker WA (ed) The microbiota in gastrointestinal pathophysiology. Elsevier, pp. 171–178. https://doi.org/10.1016/B978-0-12-804024-9.00020-3