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The Application of Lactobacillus reuteri CCM 8617 and Flaxseed Positively Improved the Health of Mice Challenged with Enterotoxigenic E. coli O149:F4

  • Z. AndrejčákováEmail author
  • D. Sopková
  • R. Vlčková
  • Z. Hertelyová
  • S. Gancarčíková
  • R. Nemcová
Article

Abstract

The aim of our study was to monitor the effects of dietary synbiotics on experimentally infected mice. Sixty mice were divided into the following three groups: negative control group C1, positive control group C2 (mice infected with enterotoxigenic Escherichia coli O149:F4NAL), and experimental group LF (Lactobacillus reuteri CCM 8617RIF + 10% flaxseed + E. coli O149:F4NAL). Supplements were administered for 42 days. Microbiological, hematological, and biochemical analyses, electrophoretic analysis of lactate dehydrogenase (LDH) isoenzymes, and analysis of fatty acids using gas chromatography and isotachophoresis were performed. We recorded higher numbers of jejunal and ileal lactic acid bacteria, lower Enterobacteriaceae counts in the feces of the animals, and an increased production of organic acids in the synbiotic-fed group. The supplements applied favored n−3 polyunsaturated fatty acid (PUFA) metabolism and inhibited n−6 PUFA metabolism; thus, they influenced the n−6 to n−3 and eicosapentaenoic to arachidonic acid ratios. Additionally, the incorporation of n−3 PUFAs to the cell membrane decreased the activity of LDH, transaminases, and alkaline phosphatase. Results obtained in our study indicate the positive effect of continuous supplementation of combination of probiotic cheese enriched with L. reuteri CCM 8617RIF and crushed flaxseed on composition of intestinal microflora and alleviation of the course of infection induced by pathogenic bacterium E. coli O149:F4NAL.

Keywords

Probiotic Flaxseed Lactobacillus E. coli Mice Infection 

Notes

Acknowledgments

We would like to thank Assoc. Prof. Stewart Odendhal, DVM, PhD., for the English correction.

Funding information

This work was financially supported by the projects of the Ministry of Education, Science, Research and Sport of the Slovak Republic No. 1/0476/16 and No. 1/0241/18.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. 1.Institute of Physiology, Department of Anatomy, Histology and PhysiologyUniversity of Veterinary Medicine and Pharmacy in KošiceKošiceSlovak Republic
  2. 2.Department of Experimental Medicine, Faculty of MedicinePavol Jozef Šafarik UniversityKošiceSlovak Republic
  3. 3.Institute of Microbiology and GnotobiologyUniversity of Veterinary Medicine and Pharmacy in KošiceKošiceSlovak Republic

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