Effect of Lactobacillus casei on Innate Immunity Responses and Aeromonas hydrophila Resistance in Shabot, Tor grypus

  • Takavar MohammadianEmail author
  • Abdolhossein Jangaran-Nejad
  • Mehrzad Mesbah
  • Tahereh Shirali
  • Pedram Malekpouri
  • Mohammad-Reza Tabandeh


For investigating the possible effect of Lactobacillus casei on the immune status of Shabot, Tor grypus, 480 fish weighing 40 ± 10 g were randomly divided into four groups in triplicate. The first group (control) was fed on a commercial diet without probiotic supplementation. Other groups (A, B, and C) were received diet supplemented by different levels of L. casei, i.e., 5 × 106, 5 × 107, and 5 × 108 CFU g−1, respectively. The feeding trial was performed for 60 continuous days followed by 15 days as withdraw period, i.e., day 75. Serum immune responses were determined on days 0, 30, 60, and 75 of feeding trial. Gene expression in the kidneys was analyzed on days 0, 60, and 75 as well as following bacterial challenge. The results showed that dietary L. casei supplementation significantly (P < 0.05) increased serum lysozyme, serum bactericidal, complement, and respiratory burst activities as compared with control group, while it was observed that treated fish did not show any significant differences in immune responses on day 75 (P < 0.05). Results of gene expression (TNF-α, IL-1β, and IL-8) showed significant increases among different experimental times (P < 0.05). According to obtained results, it might be concluded that the feeding by L. casei could likely enhance the immune responses and gene expression in T. grypus. Treatments A and C were better than B, but the lower concentration is better as an economic viewpoints and biosecurity. Therefore, concentration A is suggested.


Immune status Tor grypus Lactobacillus casei TNF-α IL-1β IL-8 


Funding Information

This work was funded by a grant from the Shahid Chamran University of Ahvaz Research Council.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Takavar Mohammadian
    • 1
    Email author
  • Abdolhossein Jangaran-Nejad
    • 1
  • Mehrzad Mesbah
    • 1
  • Tahereh Shirali
    • 1
  • Pedram Malekpouri
    • 2
  • Mohammad-Reza Tabandeh
    • 3
  1. 1.Department of Clinical Sciences, Faculty of Veterinary MedicineShahid Chamran University of AhvazAhvazIran
  2. 2.Young Researchers and Elites Club, Science and Research BranchIslamic Azad UniversityTehranIran
  3. 3.Department of Biochemistry and Molecular Biology, Faculty of Veterinary MedicineShahid Chamran University of AhvazAhvazIran

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