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The Effects of a Synbiotic Mixture of Galacto-Oligosaccharides and Bacillus Strains in Caspian Salmon, Salmo trutta caspius Fingerlings

  • Maryam AftabgardEmail author
  • Alireza Salarzadeh
  • Mahmoud Mohseni
Article

Abstract

The effect of dietary supplementation with a synbiotic mixture of galacto-oligosaccharides (GOS) and Bacillus spp. was examined in Caspian salmon, Salmo trutta caspius (Kessler, 1877) fingerlings. Caspian salmon fed with the synbiotic diet had significantly higher weight gain rate, protein efficiency ratio, and survival rate, as well as lower feed conversion ratio, compared to the control group (P < 0.05). The serum protein, albumin, globulin, and lactate dehydrogenase levels of the fish fed with the synbiotic diet were significantly higher than the control group (P < 0.05), while the serum alkaline phosphatase levels were significantly lower (P < 0.05). The activities of the innate immune response parameters, including lysozyme, superoxide dismutase, and catalase were significantly higher in the Caspian salmon fed with the synbiotic diet (P < 0.05). The gut microbiota of the Caspian salmon fed with the synbiotic diet contained significantly elevated total viable aerobic bacterial counts (TVABCs), lactic acid bacteria (LAB) levels, and LAB/TVABCs ratio (P < 0.05). Additionally, the gut activities of amylase, trypsin, and chymotrypsin in the gut, as well as the trypsin/chymotrypsin ratio, were significantly increased in the fish that received the synbiotic diet (P < 0.05). In conclusion, the combined GOS and Bacillus spp. supplement positively affected the growth, survival rate, immunobiochemical parameters, digestive activity, and beneficial microbial density in the gut of Caspian salmon fingerlings.

Keywords

Galacto-oligosaccharides Bacillus spp. Digestive enzymes Innate immunity Gut lactic acid bacteria Caspian salmon 

Notes

Acknowledgements

We are thankful to the staff at the CFRC for providing the facilities and their technical assistance for this study.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Maryam Aftabgard
    • 1
    Email author
  • Alireza Salarzadeh
    • 1
  • Mahmoud Mohseni
    • 2
  1. 1.Department of Fisheries, College of Natural Resources, Bandar Abbas BranchIslamic Azad UniversityBandar AbbasIran
  2. 2.Coldwater Fishes Research Center (CFRC), Iranian Fisheries Science Research Institute (IFSRI)Agricultural Research, Education and Extension Organization (AREEO)TonekabonIran

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