Assessing the Safety and Efficacy of Lactobacillus plantarum MTCC 5690 and Lactobacillus fermentum MTCC 5689 in Colitis Mouse Model
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Probiotic lactobacilli have an unprecedented history of safe use, although some cases of infections have raised concerns about their safety, and hence, a rigorous screening of any new strain even of Lactobacillus is a must in order to study possible adverse interactions with the host, particularly under unhealthy conditions. The present study was, therefore, undertaken to investigate the safety as well as therapeutic efficacy of probiotic Lactobacillus plantarum MTCC 5690 and L. fermentum MTCC 5689 strains in dextran sodium sulfate (DSS)-induced colitis mouse model. Both MTCC 5690 and MTCC 5689 did not induce any detrimental effect on the colitic mice, as was reflected by normal colon and caecum length, blood biochemistry, hematology, and absence of inflammation. Although translocation of both the strains was observed in extraintestinal organs, probiotic-fed mice had significantly improved intestinal permeability and decreased myeloperoxidase (MPO) activity. Probiotic interventions also led to an improved health index and better growth of colitis mice compared to colitis animals with no probiotic intervention. These results point towards the safe use of L. plantarum MTCC 5690 and L. fermentum MTCC 5689 as biotherapeutics for amelioration of inflammatory conditions after establishing their efficacy in human clinical trials.
KeywordsProbiotics Lactobacilli Colitis Safety Anti-inflammatory
The authors acknowledge the Director, ICAR-National Dairy Research Institute (NDRI), Karnal, India, for providing facilities to carry out this study. The financial support from ICAR-National Dairy Research Institute, Karnal, in terms of providing fellowship to the first author is also duly acknowledged.
Compliance with Ethical Standards
The animal studies were conducted as per current legislation on animal experiments with approval from the Institutional Animal Ethical Committee (IAEC approval no. 76/14) of Indian Council of Agricultural Research-National Dairy Research Institute, Deemed University, Karnal-132001, Haryana, India (Reg. No. 1705/GO/ac/13/CPCSA/2013).
Conflict of Interest
The authors declare that they have no conflict of interest.
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