Probiotics and Antimicrobial Proteins

, Volume 11, Issue 3, pp 981–989 | Cite as

Effects of Dietary Supplementation of Probiotic Mix and Prebiotic on Growth Performance, Cecal Microbiota Composition, and Protection Against Escherichia coli O78 in Broiler Chickens

  • Reda TarabeesEmail author
  • Khaled M. Gafar
  • Mohamed S. EL-Sayed
  • Awad A. Shehata
  • Marwa Ahmed


The current study conducted to investigate the effects of a multi-strain commercial probiotic mix and prebiotic (isomaltooligosaccharide, IMO) on broiler performance parameters, cecal microbiota composition, and protection against challenge with avian pathogenic Escherichia coli (APEC) O78. For this purpose, 101-day-old Cobb chicks were randomly allocated into four experimental groups (G)—G01: basal diet, G02: basal diet and challenged with E. coli O78 at 28 days old, G03: basal diet with probiotic mix and challenged with E. coli O78 at 28 days old, and G04: basal diet with IMO and challenged with E. coli O78 at 28 days old. Results showed that weekly body weights in G03 were heavier (P < 0.05) than those of G01 and G02 at the fourth and fifth week. The body gain at the fourth and fifth week was higher (P < 0.05) in G03 than those of the other groups. The hot carcass weight (g) was significantly higher in broiler chickens kept in G03 and G04 compared with those in the control groups (G01 and G02). The probiotic mix and IMO significantly increased the total lactobacilli and total lactobacilli-enterococci populations in the ceca of treated broilers, respectively compared with those in the control groups. The treated broilers (G03 and G04) also showed lower mortality percentage and E. coli recovery rates the liver and spleen than those in G02. It was concluded that probiotic mix or IMO significantly improved the growth performance and modulated the intestinal microbiota of broiler chickens challenged with APEC O78.


Probiotic Isomaltooligosaccharide Broilers Intestinal microbiota Escherichia coli O78 


Funding Information

The authors would like to acknowledge the Science and Technology Development Fund, Egypt, Grant No. 9222 for their partial support.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that there is no conflict of interest.


The authors would like to state that the manuscript has not been published and is not under consideration for publication elsewhere.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Reda Tarabees
    • 1
    Email author
  • Khaled M. Gafar
    • 2
  • Mohamed S. EL-Sayed
    • 1
  • Awad A. Shehata
    • 3
    • 4
  • Marwa Ahmed
    • 5
  1. 1.Department of Bacteriology, Mycology, and Immunology, Faculty of Veterinary MedicineUniversity of Sadat CitySadat CityEgypt
  2. 2.Department of Nutrition and Clinical Nutrition, Faculty of Veterinary MedicineUniversity of Sadat CitySadat CityEgypt
  3. 3.Department of Birds and Rabbit Diseases, Faculty of Veterinary MedicineUniversity of Sadat CitySadat CityEgypt
  4. 4.Faculty of Veterinary Medicine, Albrecht-Daniel-Thaer-InstituteUniversity of LeipzigLeipzigGermany
  5. 5.Directorate of Veterinary MedicineEl HamoulEgypt

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