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Probiotics and Antimicrobial Proteins

, Volume 11, Issue 3, pp 1055–1069 | Cite as

Actinomycin-Producing Endophytic Streptomyces parvulus Associated with Root of Aloe vera and Optimization of Conditions for Antibiotic Production

  • Sandhya ChandrakarEmail author
  • Ashwini Kumar Gupta
Article

Abstract

Endophytic actinomycetes are a rich source of novel antimicrobial compounds. The aim of this study was to evaluate the production of antimicrobial compound by endophytic Streptomyces sp. Av-R5 associated with root of Aloe vera against multidrug-resistant human pathogens. The 16S rRNA sequence of the isolate Av-R5 has been identified as Streptomyces parvulus NBRC 13193T (AB184326) and the sequence was submitted to the National Center for Biotechnology Information (NCBI) GenBank database (accession number KY771080). Streptomyces parvulus Av-R5 grown under submerged fermentation condition optimized by central composite design (glucose 11.16 g/L, soybean meal 10.25 g/L, sodium chloride 11.18 g/L, calcium carbonate 1.32 g/L at pH 7.19 at 31.42 °C with 6.04% seed inoculum for 10 days of incubation) exhibited the highest activity against multidrug-resistant Staphylococcus aureus JNMC-3, Staphylococcus epidermidis JNMC-4, Klebsiella pneumoniae MTCC-3384, Klebsiella pneumoniae JNMC-6, Pseudomonas aeruginosa MTCC-741, Proteus vulgaris JNMC-7, Candida albicans MTCC-183, and Aspergillus niger MTCC-872. The structures of the active compounds were elucidated by UV-Vis spectroscopy, 1H and 13C NMR, FT-IR, and ESIMS. Actinomycin D and actinomycin X were detected in crude extracts and major components were eluted by HPLC at 10.96 and 6.81 min, respectively. In this case, a high yield of actinomycin D and actinomycin X (400 mg/L) was achieved with Streptomyces parvulus Av-R5, fermented in glucose soybean meal broth media, which can be used in industrial fermentation process to obtain high yields.

Keywords

Endophytic actinomycetes Streptomyces parvulus Antimicrobial activity Optimization Multidrug-resistant pathogens 

Notes

Acknowledgements

Authors are thankful to the Head, SLS, PRSU, Raipur, for providing the necessary facilities for the research. The authors also thank Microbial Type Culture Collection and Gene Bank (MTCC), Chandigarh for providing cultures and Pandit Jawaharlal Nehru Medical College, Raipur, Chhattisgarh for providing clinical cultures for research.

Funding

This study was funded by University Grant Commission, New Delhi, India (F.7-145/2007BSR) and granted financial support from DST, New Delhi under FIST program and UGC, New Delhi for DRS-SAP III.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

12602_2018_9451_MOESM1_ESM.docx (1.8 mb)
ESM 1 (DOCX 1891 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Microbiology Research Laboratory, School of Studies in Life SciencePt. Ravishankar Shukla UniversityRaipurIndia

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