More Protection of Lactobacillus acidophilus Than Bifidobacterium bifidum Probiotics on Azoxymethane-Induced Mouse Colon Cancer
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Based on the ability of the probiotics in the gut microflora modification, they can have the beneficial effects on diseases in the short and/or the long term. In previous study, we revealed that unlike Bifidobacterium bifidum, the amount of Lactobacillus acidophilus remained almost unchanged in mice gut microflora in the long term, indicating more stability of L. acidophilus than B. bifidum which can be used to prevent some incurable diseases such as cancer. Thirty-eight male BALB/c mice were divided into four groups, control, azoxymethane (AOM), L. acidophilus, and B. bifidum probiotics, to evaluate the protective effects of the probiotics on AOM-induced mouse colon cancer. Except for the control group, the rest of the animals were weekly given AOM (15 mg/kg, s.c) in three consecutive weeks. Colon lesion incidence was 74% in the AOM group in comparison with the control (0%) (P < 0.05). The lesions were varied from mild to severe dysplasia and colonic adenocarcinoma. Administration of the probiotics inhibited the incidence of colonic lesions by about 57% in L. acidophilus (P < 0.05) and 27% in B. bifidum (P > 0.05) compared to the AOM group. The serum levels of CEA and CA19-9 tumor markers were significantly decreased in L. acidophilus in comparison with the AOM group (P < 0.05). Moreover, the serum levels of IFN-γ and IL-10 and the number of CD4+ and CD8+ cells were significantly increased in L. acidophilus compared to AOM (P < 0.05). Our study highlighted the more potential effects of L. acidophilus probiotic than B. bifidum on mouse colon cancer.
KeywordsLactobacillus acidophilus Bifidobacterium bifidum Probiotics Gut microflora Colon cancer Mice
This study was supported by Tehran University of Medical Sciences (Grant Number: 17733) and Iran University of Medical Sciences (Grant Number: 18890).
Compliance with Ethical Standards
All procedures performed in studies involving animals were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.
Conflict of Interest
The authors declare that they have no conflict of interest.
- 4.Alizadeh AM, Afrouzan H, Dinparast-Djadid N, Sawaya AC, Azizian S, Hemmati HR, Mohagheghi MA, Erfani S (2015) Chemoprotection of MNNG-initiated gastric cancer in rats using Iranian propolis. Arch Iran Med 18:18–23 doi:0151801/aim.006Google Scholar
- 14.Alizadeh AM, Mohammadghasemi F, Zendehdel K, Kamyabi-moghaddam Z, Tavassoli A, Amini-najafi F, Khosravi A (2015) Apoptotic and proliferative activity of mouse gastric mucosa following oral administration of fumonisin B1. Iran J Basic Med Sci 18:8Google Scholar
- 15.Khaniki M, Azizian S, Alizadeh AM, Hemmati H, Emamipour N, Mohagheghi MA (2013) The antiproliferative and anticancerogenic effects of nano-curcumin in rat colon cancer. Tehran Univ Med J 71:277–284Google Scholar
- 16.Fareez IM, Lim SM, Zulkefli NAA, Mishra RK, Ramasamy K (2017) Cellulose derivatives enhanced stability of alginate-based beads loaded with lactobacillus plantarum LAB12 against low pH, high temperature and prolonged storage. Probiotics & Antimicrob Prot 1–15. https://doi.org/10.1007/s12602-017-9284-8
- 18.Urbanska AM, Bhathena J, Cherif S, Prakash S (2014) Orally delivered microencapsulated probiotic formulation favorably impacts polyp formation in APC (Min/+) model of intestinal carcinogenesis. Artif Cells Nanomed Biotechnol 1–11. https://doi.org/10.3109/21691401.2014.898647
- 19.Chen CC, Lin WC, Kong MS, Shi HN, Walker WA, Lin CY, Huang CT, Lin YC, Jung SM, Lin TY (2012) Oral inoculation of probiotics Lactobacillus acidophilus NCFM suppresses tumour growth both in segmental orthotopic colon cancer and extra-intestinal tissue. Br J Nutr 107:1623–1634. https://doi.org/10.1017/s0007114511004934 CrossRefGoogle Scholar
- 20.Lee J-A, Ko J-H, Jung B-G, Kim T-H, Hong J-I, Park Y-S, Lee B-J (2013) Fermented Prunus mume with probiotics inhibits 7, 12-Dimethylbenz [a] anthracene and 12-O-Tetradecanoyl phorbol-13-acetate induced skin carcinogenesis through alleviation of oxidative stress. Asian Pac J Cancer Prev 14:2973–2978CrossRefGoogle Scholar
- 24.Mahmoodi M, Alizadeh AM, Sohanaki H, Rezaei N, Amini-Najafi F, Khosravi AR, Hosseini S-K, Safari Z, Hydarnasab D, Khori V (2012) Impact of fumonisin B1 on the production of inflammatory cytokines by gastric and colon cell lines. Iran J Allergy Asthma Immunol 11:165–173Google Scholar
- 25.Rizzo A, Pallone F, Monteleone G, Fantini MC (2011) Intestinal inflammation and colorectal cancer: a double-edged sword? World J Gastroenterol 17:3092Google Scholar
- 26.Mahmoodi M, Alizadeh AM, Amini-Najafi M, Khosravi AR, Hosseini S-K, Safari Z, Hydarnasab D (2012) Effects of fumonisin B1 on the stomach and colon cell lines in vitro. Tehran Univ Med Sci 69(12):744–753Google Scholar