Isolation and Preliminary Screening of a Weissella confusa Strain from Giant Panda (Ailuropoda melanoleuca)

  • Lvchen Xiong
  • Xueqin Ni
  • Lili Niu
  • Yi Zhou
  • Qiang Wang
  • Abdul Khalique
  • Qian Liu
  • Yan Zeng
  • Gang Shu
  • Kangcheng Pan
  • Bo Jing
  • Dong Zeng
Article
  • 10 Downloads

Abstract

Weissella confusa has recently received attention for its probiotic potential. Some W. confusa and Weissella cibaria strains isolated from fermented foods show favorable probiotic effects. However, the probiotic properties of W. confusa isolated from giant panda remain unreported to date. Thus, this study isolated a W. confusa strain from giant panda feces and then investigated its characteristics and probiotic properties. A lactic acid bacteria strain was isolated from giant panda fecal samples. The isolated strain was screened by in vitro probiotic property tests, including in vitro antimicrobial test, antioxidant test, surface hydrophobicity, and stress resistance. On the basis of biochemical identification and 16S rDNA sequencing, the W. confusa strain was identified as BSP201703. This Weissella confusa strain can survive at pH 2 and 0.3% (w/v) concentration of bile salt environment and inhibit common intestinal pathogens. It also possesses an in vitro antioxidant capacity, a high auto-aggregation ability, and a high surface hydrophobicity. BSP201703 might serve as a probiotic to giant pandas.

Keywords

Giant panda Weisslella confusa Probiotic properties Screening 

Notes

Acknowledgements

The present study was supported by National Natural Science Foundation of China (No. 31672318) and the Funded Project of Chengdu giant panda Breeding Research Foundation (No. CPF2014-15 and CPF2015-16).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Animal Microecology Institute, College of Veterinary MedicineSichuan Agricultural UniversityChengduChina
  2. 2.Chengdu Wildlife Institute, Chengdu ZooChengduChina
  3. 3.Key laboratory of Animal Disease and Human Health of Sichuan ProvinceChengduChina

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