Probiotics and Antimicrobial Proteins

, Volume 11, Issue 1, pp 256–263 | Cite as

Assessment of Plant-Probiotic Performance of Novel Endophytic Bacillus sp. in Talc-Based Formulation

  • Jasim Basheer
  • Aswani Ravi
  • Jyothis Mathew
  • Radhakrishnan Edayileveettil KrishnankuttyEmail author


Endophytic bacteria are considered to have a plethora of plant growth promoting and anti-phytopathogenic traits to live within the plants. Hence, they have immense promises for plant probiotic development. In the current study, plant probiotic endophytic Bacillus sp. CaB5 which has been previously isolated from Capsicum annuum was investigated for its performance in talc-based formulation. For this, CaB5 was made into formulation with sterile talc, calcium carbonate, and carboxymethyl cellulose. The viability analysis of the formulation by standard plate count and fluorescence methods has confirmed the stable microbial count up to 45 days. Plant probiotic performance of the prepared formulation was analyzed on cowpea (Vigna unguiculata) and lady’s finger (Abelmoschus esculentus). The results showed the formulation treatment to have enhancement effect on seed germination as well as plant growth in both selected plants. The results highlight the potential of CaB5-based formulation for field application to enhance growth of economically important plants.


Bacillus sp. Talc Biofertilizer Formulation preparation Fluorescence microscopy 


Funding Information

This study was supported by Kerala State Council Science Technology and Environment (KSCSTE) under KSCSTE-SARD Programme and KSCSTE-SRS project, Department of Biotechnology (DBT), Government of India under DBT-RGYI, and DBT-MSUB support scheme (BT/PR4800/INF/22/152/2012 dated 23.03.2012).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Jasim Basheer
    • 1
  • Aswani Ravi
    • 1
  • Jyothis Mathew
    • 1
  • Radhakrishnan Edayileveettil Krishnankutty
    • 1
    Email author
  1. 1.School of BiosciencesMahatma Gandhi University, PD Hills (PO)KottayamIndia

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