Ocean Science Journal

, Volume 54, Issue 1, pp 129–139 | Cite as

Physiological Responses of Cultured Seaweed Pyropia yezoensis to Phosphorous Limitation in the Nakdong River Estuary, Korea

  • Sangil Kim
  • Sang Chol Yoon
  • Man Ho Yoo
  • Kyung Woo Park
  • Sang Rul Park
  • Seok-Hyun YounEmail author


Pyropia yezoensis cultured in the estuary of the Nakdong River in Korea is an important fisheries resource for the local economy. Over the past few decades, the environmental conditions of this estuary have undergone marked changes due to coastal development, and particularly so since the construction of weirs in the main stream of the Nakdong River in 2012, which has resulted in changes in seawater nutrient concentrations. Although the production of P. yezoensis has declined notably since 2012, little is known regarding the relationship between this decline and the environmental changes. In this study, during the period from February to March 2017, we investigated environmental parameters and physiological characteristics of the cultured P. yezoensis in the Nakdong River Estuary in order to determine the factors limiting the growth of this macroalga. Water temperature, salinity, and irradiance at estuary sites were found to be favorable for Pyropia culture. High nitrogen (N) and low phosphorus (P) concentrations were measured in the sea water, which has led to a high N:P ratio. Comparable trends were observed in the N, P, and photosynthetic pigment contents of P. yezoensis tissues. Our results indicate that a deficiency in P might be a factor limiting the growth of Pyropia cultured in the Nakdong River Estuary. This study provides valuable information for gaining an understanding of the impact of environmental changes on the production of Pyropia culture in the Nakdong River Estuary.


Pyropia yezoensis Nakdong River estuary limiting factor phosphorus limitation 


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  1. Atkinson MJ, Smith SV (1983) C:N:P ratios of benthic marine plants. Limnol Oceanogr 28:568–574CrossRefGoogle Scholar
  2. Bae S-H (1991) The origin and development process of laver culture industry in Korea 1. Laver culture history till the end of Chosun dynasty. Bull Korean Fish Soc 24:153–166 (in Korean)Google Scholar
  3. Carruthers TJB, Longstaff BJ, Dennison WC, Abal EG, Aioi K (2001) Measurement of light penetration in relation to seagrass. In: Short FT, Coles RG, Short CA (eds) Global seagrass research methods. Elsevier Science, Amsterdam, pp 369–392CrossRefGoogle Scholar
  4. Chai C, Yu Z, Shen Z, Song X, Cao X, Yao Y (2009) Nutrient characteristics in the Yangtze River Estuary and the adjacent East China Sea before and after impoundment of the Three Gorges Dam. Sci Total Environ 407:4687–4695CrossRefGoogle Scholar
  5. Chang S-D, Chin P, Park K-Y (1983) Effects of temperature, salinity, and silt and clay on the rate of photosynthesis of laver, Porphyra yezoensis. Bull Korean Fish Soc 16:335–340 (in Korean)Google Scholar
  6. Chapman HD, Pratt PF (1961) Methods of analysis for soils, plants, and waters. Soil Sci 93:68CrossRefGoogle Scholar
  7. Choe S, Chung TW (1972) Nutrients and suspended organic particulates in the Estuary of Nak-Dong River. J Korean Soc Oceanogr 7:1–14 (in Korean)Google Scholar
  8. Cochlan WP, Herndon J, Kudela RM (2008) Inorganic and organic nitrogen uptake by the toxigenic diatom Pseudo-nitzschia australis (Bacillariophyceae). Harmful Algae 8:111–118CrossRefGoogle Scholar
  9. Erzini K (2005) Trends in NE Atlantic landings (southern Portugal): identifying the relative importance of fisheries and environmental variables. Fish Oceanogr 14:195–209CrossRefGoogle Scholar
  10. FAO (2018) Global aquaculture production. Food and Agriculture Organization of the United Nations. Accessed 24 Feb 2018Google Scholar
  11. García-Sánchez MJ, Fernández JA, Niell FX (1996) Photosynthetic response of P-deficient Gracilaria tenuistipitata under two different phosphate treatments. Physiol Plantarum 96:601–606CrossRefGoogle Scholar
  12. Gong G-C, Chang J, Chiang K-P, Hsiung T-M, Hung C-C, Duan S-W, Codispoti LA (2006) Reduction of primary production and changing of nutrient ratio in the East China Sea: Effect of the Three Gorges Dam? Geophys Res Lett 33:L07610. doi:10.1029/2006GL025800CrossRefGoogle Scholar
  13. Gordillo FJL (2012) Environment and algal nutrition. In: Wiencke C, Bischof K (eds) Seaweed biology. Novel insights into ecophysiology, ecology and utilization. Springer, Berlin, pp 67–86Google Scholar
  14. Hafting JT (1999) Effect of tissue nitrogen and phosphorus quota on growth of Porphyra yezoensis blades in suspension cultures. Hydrobiologia 398/399:205–314CrossRefGoogle Scholar
  15. Hanisak MD (1983) The nitrogen relationships of marine macroalgae. In: Carpenter EJ, Capone DG (eds) Nitrogen in the marine environment. Academic Press, New York, pp 699–730CrossRefGoogle Scholar
  16. Harrison PJ, Hurd CL (2001) Nutrient physiology of seaweeds: application of concepts to aquaculture. Cah Biol Mar 42:71–82Google Scholar
  17. He P, Xu S, Zhang H, Wen S, Dai Y, Lin S, Yarish C (2008) Bioremediation efficiency in the removal of dissolved inorganic nutrients by the red seaweed, Porphyra yezoensis, cultivated in the open sea. Water Res 42:1281–1289CrossRefGoogle Scholar
  18. Hein M, Pedersen MF, Sand-Jensen K (1995) Size-dependent nitrogen uptake in micro- and macroalgae. Mar Ecol-Prog Ser 118:247–253CrossRefGoogle Scholar
  19. Hernández I, Corzo A, Gordillo FJ, Robles MD, Saez E, Fernández JA, Niell FX (1993) Seasonal cycle of the gametophytic from of Porphyra umbilicalis: nitrogen and carbon. Mar Ecol-Prog Ser 99:301–311CrossRefGoogle Scholar
  20. Hori Y, Mochizuki S, Shimamoto N (2008) Relationship between the discoloration of cultivated Porphyra thalli and long-term changes of the environmental factors in the northern part of Harima-Nada, eastern Seto Inland Sea, Japan. Bull Jpn Soc Fish Oceanogr 72:107–112 (in Japanese)Google Scholar
  21. Israel A, Levy I, Friedlander M (2006) Experimental tank cultivation of Porphyra in Israel. J Appl Phycol 18:235–240CrossRefGoogle Scholar
  22. Jang S-T, Kim K-C (2006) Changes of oceanographic environment in the Nakdong Estuary. J Korean Soc Oceanogr 11:11–20 (in Korean)Google Scholar
  23. Kawamura Y (2012) Nori cultivation and nitrogen budget in the inner area of Ariake Bay. Aquabiology 34:142–148 (in Japanese)Google Scholar
  24. Kim KC, Kim JJ, Kim YE, Han KM (1996) Outflow characteristics of Nakdong River plume. J Kor Soc Coast Ocean Eng 8:305–313 (in Korean)Google Scholar
  25. KMI (2018a) Statistics–Pyropia/Porphyra. Fisheries Outlook Center, Korea Marine Institute. Accessed 24 Feb 2018Google Scholar
  26. KMI (2018b) Monthly report–seaweed. Fisheries Outlook Center, Korea Marine Institute. Accessed 24 Feb 2018Google Scholar
  27. Kwon J-N, Shim J, Lee SY, Cho JD (2013) Effects of meteorological and oceanographic properties on variability of laver production at Nakdong River Estuary, south coast of Korea. Kor J Fish Aquat Sci 46:868–877 (in Korean)Google Scholar
  28. Lobban CS, Harrison PJ (1994) Seaweed biology and ecology. Cambridge University Press, New York, 366 pGoogle Scholar
  29. Loneragan NR, Bunn SE (1999) River flow and estuarine ecosystem: implication for coastal fisheries from a review and a case study of Logan River, southeast Queensland. Aust J Ecol 24:431–440CrossRefGoogle Scholar
  30. Manabe T, Ishio S (1991) Bloom of Coscinodiscus wailesii and DO deficit of bloom in Seto Inland Sea. Mar Pollut Bull 23:181−184CrossRefGoogle Scholar
  31. Mizuta H, Shirakura Y, Yasui H (2002) Relationship between phycoerythrin and nitrogen content in Gloiopeltis furcata and Porphyra yezoensis. Algae 17:89–93CrossRefGoogle Scholar
  32. Morais P, Chícharo MA, Chícharo L (2009) Changes in a temperate estuary during the filling of the biggest European dam. Sci Total Environ 407:2245–2259CrossRefGoogle Scholar
  33. Nishikawa T, Hori Y, Tanida K, Imai I (2007) Population dynamics of the harmful diatom Eucampia zodiacus Ehrenberg causing bleachings of Porphyra thalli in aquaculture in Harima-Nada, the Seto Inland Sea, Japan. Harmful Algae 6:763–773CrossRefGoogle Scholar
  34. Nishikawa T, Tarutani K, Yamamoto T (2010) Nitrate and phosphate uptake kinetics of the harmful diatom Coscinodiscus wailesii, a causative organism in the bleaching of aquacultured Porphyra thalli. Harmful Algae 9:563–567CrossRefGoogle Scholar
  35. Park S, Lee J, Choi JU, Heo N, An S (2016) Study on the long-term changes in water quality and benthic ecology and evaluation on effect of the barrage in Nakdong River estuary. J Wetl Res 18:58–67 (in Korean)CrossRefGoogle Scholar
  36. Pedersen MF, Borum J, Fotel FL (2010) Phosphorus dynamics and limitation of fast- and slow-growing temperate seaweeds in Oslofjord, Norway. Mar Ecol-Prog Ser 399:103–115CrossRefGoogle Scholar
  37. Pereira R, Kraemer G, Yarish C, Sousa-Pinto I (2008) Nitrogen uptake by gametophytes of Porphyra dioica (Bangiales, Rhodophyta) under controlled-culture conditions. Eur J Phycol 43:107–118CrossRefGoogle Scholar
  38. Ribeiro ALNL, Tesima KE, Souza, JMC, Yokoya NS (2013) Effects of nitrogen and phosphorus availabilities on growth, pigment, and protein contents in Hypnea cervicornis J. Agardh (Gigartinales, Rhodophyta). J Appl Phycol 25:1151–1157CrossRefGoogle Scholar
  39. Sampath-Wiley P, Neefus CD (2007) An improved method for estimating R-phycoerythrin and R-phycocyanin contents from crude aqueous extracts of Porphyra (Bangiales, Rhodophyta). J Appl Phycol 19:123–129CrossRefGoogle Scholar
  40. Shim J, Hwang JR, Lee SY, Kwon J-N (2014) Variations in nutrients & CO2 uptake rates of Porphyra yezoensis Ueda and a simple evaluation of in situ N & C demand rates at aquaculture farms in South Korea. Korean J Environ Biol 32:297–305 (in Korean)CrossRefGoogle Scholar
  41. Tantanasarit C, Englande A, Babel S (2013) Nitrogen, phosphorus and silicon uptake kinetics by marine diatom Chaetoceros calcitrans under high nutrient concentrations. J Exp Mar Biol Ecol 446:67–75CrossRefGoogle Scholar
  42. Wang W-J, Sun X-T, Liu F-L, Liang ZR, Zhang J-H, Wang FJ (2016) Effect of abiotic stress on the gametophyte of Pyropia katadae var. hemiphylla (Bangiales, Rhodophyta). J Appl Phycol 28:469–479CrossRefGoogle Scholar
  43. Watanabe Y, Yamada H, Mine T, Kawamura Y, Nishihara GN, Terada R (2016) Photosynthetic responses of Pyropia yezoensis f. narawaensis (Bangiales, Rhodophyta) to a thermal and PAR gradient vary with the life-history stage. Phycologia 55:665–672CrossRefGoogle Scholar
  44. Wellburn AR (1994) The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144:307–313CrossRefGoogle Scholar
  45. Wheeler PA, Björnsäter BR (1992) Seasonal fluctuations in tissue nitrogen, phosphorus, and N:P for five macroalgal species common to the Pacific northwest coast. J Phycol 28:1–6CrossRefGoogle Scholar
  46. Wu CY, Zhang YX, Li RZ, Penc ZS, Zhang YF, Liu QC, Zhang JP, Fan X (1984) Utilization of ammonium-nitrogen by Porphyra yezoensis and Gracilaria verrucosa. Hydrobiologia 116/117:475–477Google Scholar
  47. Yamamoto M, Watanabe Y, Kinoshita H (1991) Effects of water temperature on the growth of red alga Porphyra yezoensis form narawaensis (nori) cultivated in an outdoor raceway tank. Nippon Suisan Gakk 57:2211–2217 (in Japanese)CrossRefGoogle Scholar
  48. Yoo SG (2003) Introduction to aquaculture. Gu-Deok Press, Busan, 359 p (in Korean)Google Scholar
  49. Yoo M-H, Youn SH, Park K-W, Kim A-R, Yoon SC, Suh YS (2016) The characteristics of spatio-temporal distribution on phytoplankton in the Nakdong River estuary, during 2013–2015. J Korean Soc Mar Environ Saf 22:739–749 (in Korean)Google Scholar
  50. Yoon SC, Youn SH, Lee S-M, Yoon Y-Y (2016) Long-term variations of water quality and material flux in the Nakdong River, Korea. J Korean Soc Water Sci Technol 24:161–169 (in Korean)CrossRefGoogle Scholar
  51. Yoon SC, Youn SH, Suh YS (2017) The characteristics of spatiotemporal distribution on environmental factors after construction of artificial structure in the Nakdong River estuary. J Korean Soc Mar Environ Energy 20:1–11 (in Korean)CrossRefGoogle Scholar

Copyright information

© KSO, KIOST and Springer 2019

Authors and Affiliations

  • Sangil Kim
    • 1
  • Sang Chol Yoon
    • 1
  • Man Ho Yoo
    • 1
  • Kyung Woo Park
    • 1
  • Sang Rul Park
    • 2
  • Seok-Hyun Youn
    • 1
    Email author
  1. 1.Ocean Climate and Ecology Research DivisionNational Institute of Fisheries ScienceBusanKorea
  2. 2.Estuarine and Coastal Ecology Laboratory, Department of Marine Life SciencesJeju National UniversityJejuKorea

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