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Inhibitory effect of Fungastop and Bion against carrot soft rot caused by Sclerotinia sclerotiorum


This study was conducted to assess likely inhibitory effects of Fungastop™ and Acibenzolar-S-Methyl (Bion®) against carrot rot caused by Sclerotinia sclerotiorum during postharvest phase. The results showed that Fungastop was able to markedly decrease mycelial growth and sclerotia formation of three isolates of the pathogen. In addition, Fungastop significantly reduced myceliogenic and carpogenic germination of sclerotia in the three tested isolates of S. sclerotiorum. Fungastop showed high antifungal efficacy against carrot rot in vivo as carrots were inoculated with the pathogen 2.5 h after treatment with Bion and Fungastop. However inhibitory effect of Bion against the disease was higher than Fungastop over time. The enzymatic tests revealed that Bion was able to significantly enhance levels of enzyme activities related to disease resistance including β-N-acetyl hexosaminidase, endochitinase, chitin 1,4-β-chitobiosidase and β −1,3-glucanase six days after treatment. In addition, activities of phenylalanine ammonia lyase and peroxidase were markedly increased four days after treatment. Bion showed higher effect in increasing enzyme activities related to disease resistance than Fungastop. The treatment with Fungastop and Bion enhanced relative conductivity of S. sclerotiorum mycelia over 160 min compared with control. The treatment with Fungastop increased the cell membrane permeability of mycelia more than Bion.

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  1. Adams, P. B., & Ayers, W. A. (1979). Ecology of Sclerotinia species. Phytopathology, 69, 896–898.

  2. Ali, Z., Smith, I., & Guest, D. I. (2000). Combinations of potassium Phosphonate and bion (acibenzolar-S-methyl) reduce root infection and dieback of Pinus radiata, Banksia integrifolia and Isopogon cuneatus caused by Phytophthora cinnamomi. Australasian Plant Pathology, 29, 59–63.

  3. Amini, J. (2015). Induced resistance in potato plants against verticillium wilt invoked by chitosan and acibenzolar-S-methyl. Australian Journal of Crop Science, 9, 570–576.

  4. Araujo, L., Silva Bispo, W. M., Rios, V. S., Fernandes, S. A., & Rodrigues, F. A. (2015). Induction of the phenylpropanoid pathway by acibenzolar-S-methyl and potassium phosphite increases mango resistance to Ceratocystis fimbriata infection. Plant Disease, 99, 447–459.

  5. Bagamboula, C. F., Uyttendaele, M., & Debevere, J. (2004). Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and pcymene towards Shigella sonnei and S. flexneri. Food Microbiology, 21, 33–42.

  6. Bi, Y., Tian, S. P., Guo, Y. R., Ge, Y. H., & Qin, G. Z. (2006). Sodium silicate reduces postharvest decay on Hami melons: Induced resistance and fungistatic effects. Plant Disease, 90, 279–283.

  7. Boland, G. J., & Hall, R. (1994). Index of plant hosts of Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology, 16, 93–108.

  8. Colson-Hanks, E. S., Allen, S. J., & Deverall, B. J. (2000). Effect of 2,6-dichloroisonicotinic acid or benzothiadiazole on Alternaria leaf spot, bacterial blight and Verticillium wilt in cotton under field conditions. Australasian Plant Pathology, 29, 170–177.

  9. Cruz, M. F. A., Rodrigues, F. A., Diniz, A. P., Alves Moreira, M., & Barros, E. G. (2014). Soybean resistance to Phakopsora pachyrhizi as affected by acibenzolar-S-methyl, jasmonic acid and silicon. Journal of Phytopathology, 162, 133–136.

  10. de Capdeville, G., Wilson, C. L., Beer, S. V., & Aist, J. R. (2002). Alternative disease control agents induce resistance to blue mold in harvested ‘red delicious’ apple fruit. Phytopathology, 92, 900–908.

  11. Droby, S., Vinokur, V., Weiss, B., Cohen, L., Daus, A., Goldschmidt, E. E., & Porat, R. (2002). Induction of resistance to Penicillium digitatum in grapefruit by the yeast biocontrol agent Candida oleophila. Phytopathology, 92, 393–399.

  12. Duan, Y., Ge, C., Liu, S., Chen, C., & Zhou, M. (2013). Effect of phenylpyrrole fungicide fludioxonil on morphological and physiological characteristics of Sclerotinia sclerotiorum. Pesticide Biochemistry and Physiology, 106, 61–67.

  13. El-Ghaouth, A., Arul, J., Asselin, A., & Benhamou, N. (1992). Antifungal activity of chitosan on post-harvest pathogens: Induction of morphological and cytological alterations in Rhizopus stolonifer. Mycological Research, 9, 769–779.

  14. Elsherbiny, E. A., & Taher, M. A. (2018). Silicon induces resistance to postharvest rot of carrot caused by Sclerotinia sclerotiorum and the possible of defense mechanisms. Postharvest Biology and Technology, 140, 11–17.

  15. Finlayson, J. E., Rimmer, S. R., & Pritchard, M. K. (1989). Infection of carrots by Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology, 11, 242–246.

  16. Foster, A. J., Kora, C., Mcdonald, M. R., & Boland, G. J. (2011). Development and validation of a disease forecast model for Sclerotinia rot of carrot. Canadian Journal of Plant Pathology, 33, 187–201.

  17. Gilardi, G., Demarchi, S., Gullino, M. L., & Garibaldi, A. (2016). Evaluation of the short term effect of nursery treatments with phosphite-based products, acibenzolar-S-methyl, pelleted Brassica carinata and biocontrol agents, against lettuce and cultivated rocket Fusarium wilt under artificial inoculation and greenhouse conditions. Crop Protection, 85, 23–32.

  18. Gossen, B. D., & Rimmer, S. R. (2001). First report of resistance to benomyl fungicide in Sclerotinia sclerotiorum. Plant Disease, 85, 1206.

  19. Herrero, M.-L., Toppe, B., & Eikemo, H. (2012). Evaluation of acibenzolar-S-methyl and other low-toxicity products for control of rose powdery mildew (Podosphaera pannosa) in greenhouses. Acta Agriculturae Scandinavica, Section B - Soil and Plant Science, 62, 666–671.

  20. Hildebrand, P. D., Forney, C. F., Song, J., Fan, L., & McRae, K. B. (2008). Effect of a continuous low ozone exposure (50 nL L−1) on decay and quality of stored carrots. Postharvest Biology and Technology, 49, 397–402.

  21. Huang, H. C., & Kozub, G. C. (1993). Influence of inoculum production temperature on carpogenic germination of sclerotia of Sclerotinia sclerotiorum. Canadian Journal of Microbiology, 39, 548–550.

  22. Iscan, G., Kirimer, N., Kurkcuoglu, M., Baser, K. H. C., & Demirci, F. (2002). Antimicrobial screening of Mentha piperita essential oils. Journal of Agricultural and Food Chemistry, 50, 3943–3946.

  23. Keinath, A. P., Wechter, W. P., & Farnham, M. W. (2016). Suppression of Bacterial Blight on Mustard Greens with Host Plant Resistance and Acibenzolar-S-Methyl. Plant Disease, 100, 1921–1926.

  24. Kora, C., McDonald, M. R., & Boland, G. J. (2003). Sclerotinia rot of carrot: An example of phonological adaptation and bicyclic development of Sclerotinia sclerotiorum. Plant Disease, 87, 456–470.

  25. Kora, C., McDonald, M. R., & Boland, G. J. (2005). Epidemiology of sclerotinia rot of carrot caused by Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology, 27, 245–258.

  26. Lambert, R. J. W., Skandamis, P. N., Coote, P. J., & Nychas, G. J. E. (2001). A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. Journal of Applied Microbiology, 91, 453–462.

  27. Martínez-Romero, D., Serrano, M., Bailén, G., Guillén, F., Zapata, P. J., Valverde, J. M., Castillo, S., Fuentes, M., & Valero, D. (2008). The use of a natural fungicide as an alternative to preharvest synthetic fungicide treatments to control lettuce deterioration during postharvest storage. Postharvest Biology and Technology, 47, 54–60.

  28. Molina, M., & Giannuzzi, L. (1999). Combined effect of temperature and propionic acid concentration on the growth of Aspergillus parasiticus. Food Research International, 32, 677–682.

  29. Molloy, C., Cheah, L., & Koolaard, J. (2004). Induced resistance against Sclerotinia sclerotiorum in carrots treated with enzymatically hydrolysed chitosan. Postharvest Biology and Technology, 33, 61–65.

  30. Nascimento, K. J. T., Leonardo, A., Resende, R. S., Schurt, D. A., Silva, W. L. D., & Rodrigues, F. D. A. (2016). Silicon, acibenzolar-S-methyl and potassium phosphite in the control of brown spot in rice. Bragantia, 75, 212–221.

  31. Ojaghian, M. R., Almoneafy, A. A., Cui, Z. Q., Xie, G. L., Zhang, J.-Z., Shang, C., & Li, B. (2013). Application of acetyl salicylic acid and chemically different chitosans against storage carrot rot. Postharvest Biology and Technology, 8, 51–60.

  32. Ojaghian, M. R., Chen, Y., Chen, S., Cui, Z.-Q., Xie, G.-L., & Zhang, J.-Z. (2014). Antifungal and enzymatic evaluation of plant crude extracts derived from cinnamon and rosemary against Sclerotinia carrot rot. Annals of Applied Biology, 164, 415–429.

  33. Ojaghian, M.R., Cui, Z-q., Xie, G-L., Li, B., &, Zhang, J. (2012b). Brassica green manure rotation crops reduce potato stem rot caused by Sclerotinia sclerotium. Australasian Plant Pathology, 41, 347–349.

  34. Ojaghian, M. R., Heng, J., Xie, G.-L., Cui, Z.-q., Zhang, J., & Li, B. (2012a). In vitro biofumigation of Brassica tissues against potato stem rot caused by Sclerotinia sclerotiorum. Plant Pathology Journal, 28, 185–190.

  35. Ojaghian, M. R., Wang, L., Xie, G.-L., & Zhang, J.-Z. (2018). Increased resistance against storage rot in transgenic carrots expressing chitinase chit42 from Trichoderma harzianum. Scientia Horticulturae, 234, 81–86.

  36. Ojaghian, M. R., Wang, Q., Li, X., Sun, X., Xie, G.-L., Zhang, J.-Z., Fan, H.-W., & Wang, L. (2016). Inhibitory effect and enzymatic analysis of E-cinnamaldehyde against sclerotinia carrot rot. Pesticide Biochemistry and Physiology, 127, 8–14.

  37. Ojaghian, M. R., Zhang, Z.-J., Xie, G.-L., Wang, Q., Li, X. L., & Guo, D.-P. (2017). Efficacy of UV-C radiation in inducing systemic acquired resistance against storage carrot rot caused by Sclerotinia sclerotiorum. Postharvest Biology and Technology, 130, 94–102.

  38. Parkinson, L. E., Crew, K. S., Thomas, J. E., & Dann, E. K. (2015). Efficacy of acibenzolar-S-methyl (bion®) treatment of Australian commercial passionfruit, Passiflora edulis f. sp. flavicarpa, on resistance to Passionfruit woodiness virus (PWV) and activities of chitinase & β-1,3-glucanase. Australasian Plant Pathology, 44, 311–318.

  39. Porat, R., Vinokur, V., Weiss, B., Cohen, L., Daus, A., Goldschmidt, E. E., & Droby, S. (2003). Induction of resistance to Penicillium digitatum in grapefruit by β-aminobutyric acid. European Journal of Plant Pathology, 109, 901–907.

  40. Rios, J. A., Rodrigues, F. A., Debona, D., Resende, R. S., Moreira, W. R., & Andrade, C. C. L. (2014). Induction of resistance to Pyricularia oryzae in wheat by acibenzolar-S-methyl, ethylene and jasmonic acid. Tropical Plant Pathology, 39, 224–233.

  41. Roberti, R., Veronesi, A., Cesari, A., Cascone, A., Di Berardino, I., Bertini, L., & Caruso, C. (2008). Induction of PR proteins and resistance by the biocontrol agent Clonostachys rosea in wheat plants infected with Fusarium culmorum. Plant Science, 175, 339–347.

  42. Rubatzky, V. E., Quiros, C. F., & Simon, P. W. (1999). Carrots and related vegetable Umbelliferae. New York, USA: CABI Publishing.

  43. Ryan, C. A., & Farmer, E. F. (1991). Oligsaccharide signals in plants: A current assessment. Annual Review of Plant Biology, 42, 651–674.

  44. Sarwar, N., Hayat, Z. C. H. M., Haq, I., & Jamil, F. F. (2005). Induction of systemic resistance in chickpea against Fusarium wilt by seed treatment with salicylic acid and bion. Pakistan Journal of Botany, 37, 989–995.

  45. Schouteden, N., Lemmens, E., Stuer, N., Curtis, R., Panis, B., & Waele, D. D. (2017). Direct nematicidal effects of methyl jasmonate and acibenzolar-S-methyl against Meloidogyne incognita. Natural Product Research, 31, 1219–1222.

  46. Sun, Y., Wang, Y., Xie, Z., Guo, E., Han, L., Zhang, X., & Feng, J. (2017). Activity and biochemical characteristics of plant extract cuminic acid against Sclerotinia sclerotiorum. Crop Protection, 101, 76–83.

  47. Tahvonen, R. (1985). The prevention of Botrytis cinerea and Sclerotinia sclerotiorum on carrots during storage by spraying the tops with fungicide before harvesting. Annales Agrigulturae Fenniae, 24, 89–95.

  48. Tripathi, D., & Pappu, H. R. (2015). Evaluation of acibenzolar-S-methyl-induced resistance against Iris Yellow Spot Tospovirus. European Journal of Plant Pathology, 142, 855–864.

  49. Wang, Y., Duan, Y., Wang, J., & Zhou, M. (2015). A new point mutation in the iron-sulfur subunit of succinate dehydrogenase confers resistance to boscalid in Sclerotinia sclerotiorum. Molecular Plant Pathology, 16, 653–661.

  50. Wu, B. M., & Subbarao, K. V. (2008). Effects of soil temperature, moisture and burial depths on carpogenic germination of Sclerotinia sclerotiorum and S.minor. Phytopathology, 98, 1144–1152.

  51. Xu, C., Hou, Y., Wang, J., Yang, G., Liang, X., & Zhou, M. (2014). Activity of a novel strobilurin fungicide benzothiostrobin against Sclerotinia sclerotiorum. Pesticide Biochemistry and Physiology, 115, 32–38.

  52. Yigit, F. (2011). Acibenzolar-S-methyl induces lettuce resistance against Xanthomonas campestris pv. vitians. African Journal of Biotechnology, 10, 9606–9612.

  53. Yu, C., Zeng, L., Sheng, K., Chen, F., Zhou, T., Zheng, X., & Yu, T. (2014). γ-Aminobutyric acid induces resistance against Penicillium expansum by priming of defence responses in pear fruit. Food Chemistry, 159, 29–37.

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The authors thank Dr. Zhou-Qi Cui for her technical supports and advices to improve this article. I gratefully appreciate the members of Plant Pathology Lab., Zhejiang University for help with this research. This research was funded by Ton Duc Thang University, Ho Chi Minh City, Vietnam.

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Correspondence to Seyedmohammadreza Ojaghian.

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Ojaghian, S., Wang, L., Zhang, J. et al. Inhibitory effect of Fungastop and Bion against carrot soft rot caused by Sclerotinia sclerotiorum. Phytoparasitica 48, 95–106 (2020).

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  • Carpogenic
  • Endochitinase
  • POD
  • PPO
  • S. Sclerotiorum