Genotypic and phenotypic characterization of Phytophthora infestans populations from potato in Turkey
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Late blight disease caused by Phytophthora infestans is an important yield reducing, prolific, and destructive pathogen of Solanaceae family members, mainly tomato and potato. P. infestans may cause entire crop loss unless controlled by chemical and cultural control measures. The Cukurova region of Turkey is one of the main potato-producing areas of Turkey and climatic conditions incite P. infestans to develop and cause severe yield losses in the region. A total of 186 isolates of P. infestans were obtained through survey studies conducted during the 2013–2014 potato-growing seasons in the Cukurova region comprised of Hatay, Adana and Mersin provinces in Turkey. All the isolates were analyzed for their metalaxyl resistance, mating type, mitochondrial DNA (mtDNA) haplotype and allozyme genotype diversity. P. infestans isolates were metalaxyl sensitive and both mating types were found in the potato-growing areas of the Cukurova region. The A1 mating type was more common (68.8%) than A2 (22.5%) and 8.6% of the P. infestans isolates were self-compatible. mtDNA haplotypes were diverse in the region and Ia was the most common type. Allozyme analyses revealed that the US-1 (Gpi 86/100) and US-6 (Gpi 100/100) patterns were prevalent in the potato-growing areas of the Cukurova region. This study contains the first data on molecular and biochemical characterization of potato late blight in Turkey.
KeywordsCukurova region Potato Phytopthora infestans Mating types Metalaxyl resistance mtDNA Allozyme genotype
This study is a part of the project fully supported by the Scientific and Technical Research Council of Turkey (TUBITAK) with project number, TOVAG-112O112.
- Carter, D. A., Archer, S. A., Buck, K. W., Shaw, D. S., & Shattock, R. C. (1991). DNA polymorphisms in Phytophthora infestans the U.K. experience (pp. 272–294). Cambridge University Press.Google Scholar
- Caten, C. E., & Jinks, J. L. (1968). Spontaneous variability of single isolates of Phytophthora infestans. I. Cultural variation. Canadian Journal of Botany, 46(4), 329–348.Google Scholar
- Chowdappa, P., Kumar, N. B. J., Madhura, S., Kumar, M. S. P., Myers, K. L., Fry, W. E., Squire, J. S., & Cooke, D. (2013). Emergence of 13_A2 blue lineage of Phytophthora infestans was responsible for severe outbreaks of late blight on tomato in south-west India. Journal of Phytopathology, 161, 49–58.CrossRefGoogle Scholar
- Cooke, D. E. L., Cano, L. M., Raffaele, S., Bain, R. A., Cooke, L. R., Etherington, G. J., Deahl, K. L., Farrer, R. A., Gilroy, E. M., Goss, E. M., Grünwald, N. J., Hein, I., MacLean, D., & Kamoun, S. (2012). Genome analyses of an aggressive and invasive lineage of the Irish potato famine pathogen. PLoS Pathogens. https://doi.org/10.1371/journal.ppat.1002940.
- Danies, G., Small, I. M., Myers, K., Childers, R., & Fry, W. E. (2013). Phenotypic characterization of recent clonal lineages of Phytophthora infestans in the United States. Plant Disease, 97(7), 873–881.Google Scholar
- Dey, T., Saville, A., Myers, K., Tewari, S., Cooke, D., Tripaty, S., Fry, W., Ristaino, J. B., & Roy, S. (2018). Large sub-clonal variation in Phytophthora infestans from recent severe late blight epidemics in India. Article in Scientific Reports. February, 2018, 4429. https://doi.org/10.1038/s41598-018-22192-1.Google Scholar
- Drenth, A., Goodwin, S. B., Fry, W. E., & Davidse, L. C. (1993). Genotypic diversity of Phytophthora infestans in The Netherlands revealed by DNA polymorphisms. Phytopathology, 83(10), 1087–1092.Google Scholar
- Erselius, L.J., Hohl, H.R., Ordonez, M.E., Oyarzun, P.J., Jarrin, F., Velasco, A., Ramon, M.P, Forbes, G.A., (1998). Genetic diversity among isolates of from various host in Ecuador, CIP program report 1997–1998.Google Scholar
- Flier, W. G., Kroon, L. P. N. M., Hermansen, A., van Raaij, H. M. G., Speiser, B., Tamm, L., Fuchs, J. G., Lambion, J., Razzaghian, J., Andrivon, D., Wilcockson, S., & Leifert, C. (2007). Genetic structure and pathogenicity of populations of Phytophthora infestans from organic potato crops in France, Norway, Switzerland and the United Kingdom. Plant Pathology, 56, 562–572.CrossRefGoogle Scholar
- Fry, W. E., Goodwin, S. B., Dyer, A. T., Matusazak, J. M., Drenth, A., Tooley, P. W., Sujkowski, L. S., Koh, Y. J., Cohen, B. A., Spielman, L. J., Deahl, K. L., & Inglis, D. A. (1993). Historical and recent migrations of Phytophthora infestans: Chronology, pathways, and implications. Plant Disease, 77, 653–661.CrossRefGoogle Scholar
- Fry, W. E., Birch, P. R. J., Judelson, H. S., Grünwald, N. J., Danies, G., Everts, K. L., Gevens, A. J., Gugino, B. K., Johnson, D. A., Johnson, S. B., McGrath, M. T., Myers, K. L., Ristaino, J. B., Roberts, P. D., Secor, G., & Smart, C. D. (2015). Five reasons to consider Phytophthora infestans a re-emerging pathogen. Phytopathology, 105, 966–981.Google Scholar
- Galindo, J., & Gallegly, M. E. (1960). The nature of sexuality in Phytophthora infestans. Phytopathology, 50, 123–128.Google Scholar
- Griffith, G. W., & Shaw, D. S. (1998). Polymorphisms in Phytophthora infestans: Four mitochondrial haplotypes are detected after PCR amplification of DNA from pure cultures or from host lesions. Appl. Environ. Microbiology, 64(10), 4007.Google Scholar
- Li, Y., van-der Lee, T., Zhu, J. H., Jind, G. H., Lane, C. Z., Zhu, S. X., Zhang, R. F., Liu, B. W., Zhao, Z. J., Kessel, G., Huanga, S. W., & Jacobsen, E. (2013). Population structure of Phytophthora infestans in China – geographic clusters and presence of the EU genotype Blue_13. Plant Pathology., 62, 932–942.CrossRefGoogle Scholar
- Njoroge, A. W., Andersson, B., & Lees, A. K. (2018). Genotyping of Phytophthora infestans in eastern-Africa reveals a dominating invasive European lineage. Phytopathology. https://doi.org/10.1094/phyto-07-18-0234-r.
- Rekad, F. Z., Cooke, L., Puglısı, I., Randall, E., Guenaouı, Y., Bouznad, Z., Evolı, M., Pane, A., Schena, L., Di Sanlio, G. M., & Caccıola, S. A. (2017). Characterization of Phytophthora infestans populations in northwestern Algeria during 2008-2014. Fungal Biology, 467–477.Google Scholar
- Statsyuk, N.V., Kuznetsova, I.N., Kozlovskaya, B.E., Kozlovsky, S.N., Elansky, E.V., Valeva, E.V., Flippov, A.V. (2010). Characteristics of the Phytophthora infestans population in Russia. Twelfth EuroBlight workshop in France. EPPO. Special Report no.14,247–254.Google Scholar
- Sujkowski, L. S., Goodwin, S. B., Dyer, A. T., & Fry, W. E. (1994). Increased genotypic diversity via migration and possible occurrence of sexual reproduction of Phytophthora infestans in Poland. Phytopathology, 84, 201–207.Google Scholar
- Tosun, N., Yıldırım, A., Türküsay, H., & Tanyolaç, B. (2007). Genetic variation among Phytophthora infestans (tomato blight) isolates from Western Turkey revealed by inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) markers. Pakistan Journal of. Botonical, 39(3), 897–902, 2007.Google Scholar
- TUIK (2015). http://www.tuik.gov.tr. Accessed 23 Aug 2018.
- TUIK (2016). http://www.tuik.gov.tr. Accessed 23 Aug 2018.
- Wang, J., Fernández-Pavía, S. P., Larsen, M. M., Garay-Serrano, E., Gregorio-Cipriano, R., Rodríguez-Alvarado, G., Grünwald, N. J., & Goss, E. M. (2017). High levels of diversity and population structure in the potato late blight pathogen at the Mexico Centre of origin. Molecular Ecology, 26, 1091–1107.CrossRefGoogle Scholar
- Yoshida, K., Schuenemann, J. W., Cano, L. M., Pais, M., Mishra, B., Sharma, R., Lanz, C., Martin, F. N., Kamoun, S., Krause, J., Thines, N., Weige, D., & Burbano, H. A. (2013). The rise and fall of the Phytophthora infestans lineage that triggered the Irish potato famine. eLife, 2, e00731. https://doi.org/10.7554/eLife.00731.CrossRefGoogle Scholar