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Toxicity Analysis of Ferterra (Chlorantraniliprole 0.4% GR) on Tilapia (Oreochromis niloticus (Linn.): Histological and Ultrastructural Observations

  • Arnab Kumar Mondal
  • Kaushik Goswami
  • Santu Ghosh
  • Sandipan Pal
  • Aloke Kumar Mukherjee
  • Palas Samanta
  • Debraj Kole
  • Apurba Ratan GhoshEmail author
Research Article
  • 4 Downloads

Abstract

Oreochromis niloticus (Linn.) was treated with different doses such as 10% (0.044 g, i.e. T1), 20% (0.088 g i.e. T2) and 30% (0.132 g i.e. T3) of LC50 value (11.008 mg/l) of Ferterra (Chlorantraniliprole 0.4% GR) for 15 days and the effects were investigated through Haematoxylin–Eosin and scanning electron microscopy method. Histological observations revealed that at T2 level, primary gill lamellae showed sharp distortions than T1; but at T3 condition vacuolations and fusion of primary and secondary gill lamellae were more prominent. Intestinal changes in the columnar epithelial cells and basement membranes were more severe at T2, and at T3 level the villi became blunt and columnar epithelial cells became fused to form a mass. In liver, disarray of cords of hepatocytes, degeneration and destruction of acinar cells were prominent at T2 and T3. In kidney, at T2 level the proximal, distal and collecting tubules were malformed, but at T3 the tubules showed shrinkage and disorganised glomerulus. Topologically, distortion and fragmentation of microridges including double-ridged structures of the gill lamellae were of serious concerns at T3 level. Intestine revealed damage of compactly arranged microvilli followed by disorientation and destruction of CEC at T3 level. Serious damage in the hepatocytes along with topological necrosis at T3 level was very prominent. Kidney was the most affected region at T2 and T3 showing shrinkage and degeneration of glomeruli, vacuolation and distortion of PCT, DCT and the haemopoietic tissue of the trunk kidney. The present study has been able to establish the dose-dependent tissue responses of Ferterra, therefore, the judicial use of it may be recommended for the sustenance of aquatic ecosystem.

Keywords

Ferterra Oreochromis niloticus Histology SEM Gill Intestine Liver Kidney 

Notes

Acknowledgements

The authors are grateful to the Head, The Department of Environmental Science, The University of Burdwan, providing instruments and laboratory facilities. We are also thankful to DST-FIST for providing fund for augmenting the infrastructural and instruments facilities in the Department of Environmental Science, through which we are benefitted a lot.

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Copyright information

© Zoological Society, Kolkata, India 2019

Authors and Affiliations

  1. 1.Ecotoxicology Laboratory, Department of Environmental ScienceThe University of Burdwan, GolapbagBurdwanIndia
  2. 2.Department of Environmental ScienceAghorekamini Prakashchandra MahavidyalayaHooghlyIndia
  3. 3.P.G. Department of Conservation Biology, Durgapur Government CollegeDurgapurIndia
  4. 4.System Toxicology Research CenterKorea Institute of ToxicologyYuseong-gu, DaejeonRepublic of Korea

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