Toxicity Analysis of Ferterra (Chlorantraniliprole 0.4% GR) on Tilapia (Oreochromis niloticus (Linn.): Histological and Ultrastructural Observations

  • Arnab Kumar Mondal
  • Kaushik Goswami
  • Santu Ghosh
  • Sandipan Pal
  • Aloke Kumar Mukherjee
  • Palas Samanta
  • Debraj Kole
  • Apurba Ratan GhoshEmail author
Research Article


Oreochromis niloticus (Linn.) was treated with different doses such as 10% (0.044 g, i.e. T1), 20% (0.088 g i.e. T2) and 30% (0.132 g i.e. T3) of LC50 value (11.008 mg/l) of Ferterra (Chlorantraniliprole 0.4% GR) for 15 days and the effects were investigated through Haematoxylin–Eosin and scanning electron microscopy method. Histological observations revealed that at T2 level, primary gill lamellae showed sharp distortions than T1; but at T3 condition vacuolations and fusion of primary and secondary gill lamellae were more prominent. Intestinal changes in the columnar epithelial cells and basement membranes were more severe at T2, and at T3 level the villi became blunt and columnar epithelial cells became fused to form a mass. In liver, disarray of cords of hepatocytes, degeneration and destruction of acinar cells were prominent at T2 and T3. In kidney, at T2 level the proximal, distal and collecting tubules were malformed, but at T3 the tubules showed shrinkage and disorganised glomerulus. Topologically, distortion and fragmentation of microridges including double-ridged structures of the gill lamellae were of serious concerns at T3 level. Intestine revealed damage of compactly arranged microvilli followed by disorientation and destruction of CEC at T3 level. Serious damage in the hepatocytes along with topological necrosis at T3 level was very prominent. Kidney was the most affected region at T2 and T3 showing shrinkage and degeneration of glomeruli, vacuolation and distortion of PCT, DCT and the haemopoietic tissue of the trunk kidney. The present study has been able to establish the dose-dependent tissue responses of Ferterra, therefore, the judicial use of it may be recommended for the sustenance of aquatic ecosystem.


Ferterra Oreochromis niloticus Histology SEM Gill Intestine Liver Kidney 



The authors are grateful to the Head, The Department of Environmental Science, The University of Burdwan, providing instruments and laboratory facilities. We are also thankful to DST-FIST for providing fund for augmenting the infrastructural and instruments facilities in the Department of Environmental Science, through which we are benefitted a lot.


  1. APHA. 2005. Standard methods for examination of water and wastewater. Washington, DC: American Public Health Association WWA.Google Scholar
  2. Arjmandi, R., M. Tavakol, and M. Shayeghi. 2011. Determination of organophosphorus insecticide residues in the rice paddies. International Journal Environmental Science Technology 7 (1): 175–182.Google Scholar
  3. Arockia, J.J., and John M.C. Mitton. 2006. Effect of carbamate pesticide lannate (methomy1) on the biochemical components of the freshwater cichlid Oreochromis mossambicus (Peters). Indian Journal of Environment and Ecoplaning 12 (1): 263–268.Google Scholar
  4. Bagheri, H., M. Saraji, M. Chitsazan, S.R. Mousavi, and M. Naderi. 2000. Mixed-level orthogonal array design for the optimization of solid-phase extraction of some pesticide from surface water. Journal of Chromatology A 888: 197–208.Google Scholar
  5. Banaee, M., A.R. Mirvaghefi, K. Ahmadi, and R. Ashori. 2009. The effect of diazinon on histophatological changes of testis and ovaries of common carp (Cyprinus carpio). Scientific Journal of Marine Biology 1 (2): 14–25.Google Scholar
  6. Banaee, M., A.R. Mirvaghefi, B. Mojazi Amiri, G.R. Rafiee, and B. Nematdost. 2011. Hematological and histopathological study of experimental diazinon poisoning in common carp fish (Cyprinus carpio). Journal of Fisheries (Iranian Journal of Natural Resources) 64 (1): 1–12.Google Scholar
  7. Banaee, M., and K. Ahmadi. 2011. Sub-lethal toxicity impacts of endosulfan on some biochemical parameters of the freshwater crayfish (Astacus leptodactylus). Research Journal of Environmental Sciences 5 (11): 827–835.Google Scholar
  8. Banaee, M. 2012. Adverse effect of insecticides on various aspects of fish’s biology and physiology. In InsecticidesBasic and Other Applications Book, Edited by Sonia Soloneski and Marcelo Larramendy, Published by In Tech Chapter 6: 101–126.Google Scholar
  9. Begum, G. 2004. Carbofuran insecticide induced biochemical alterations in liver and muscle tissues of the fish Clarias batrachus (Linn) and recovery response. Aquatic Toxicology 66 (1): 83–92.Google Scholar
  10. Butchiram, M.S., K.S. Tilak, and P.W. Raju. 2009. Studies on histopathological changes in the gill, liver and kidney of Channa punctatus (Bloch) exposed to Alachlor. Journal of Environmental Biology 30 (2): 303–306.Google Scholar
  11. Cardoso, E.L., H. Chiarini-Garcia, R.M.A. Ferreira, and C.R. Poli. 1996. Morphological changes in the gills of Lophiosilurus alexandri exposed to unionized ammonia. Journal of Fish Biology 49 (5): 778–787.Google Scholar
  12. DuPont Safety Data Sheet. 2012. DuPonta & Almix A® 20 WP; Version 2.1: Revision Date 27.07.2012, (Ref. 130000029001).Google Scholar
  13. Ghassempour, A., A. Mohammadkhah, M. Najafie, and M. Rajabzadeh. 2002. Monitoring of the pesticide diazinon in soil, stem and surface water of rice fields. Analytical Sciences: The International Journal of The Japan Society For Analytical Chemistry 18 (7): 779–783.Google Scholar
  14. Hued, A.C., S. Oberhofer, and M. de los Angeles Bistoni. 2012. Exposure to a commercial glyphosate formulation (Roundup A®) alters normal gill and liver histology and affects male sexual activity of Jenynsia multidentata (Anablepidae, Cyprinodontiformes). Archives of Environmental Contamination and Toxicology 62 (1): 107–117.Google Scholar
  15. Jipsa, J.R., and S. Logaswamy. 2013. Effect of an insecticides chlorantraniliprole on some biochemical characteristics of fish Labeo rohita. International Journal for Life Science and Educational Research 1 (3): 128–130.Google Scholar
  16. Jiraungkoorskul, W., E.S. Upatham, M. Kruatrachue, S. Sahaphong, and S. Vichasri-Grams. 2003. Biochemical and histopathological effects of glyphosate herbicide on Nile tilapia (Oreochromis niloticus). Environmental Toxicology 18 (4): 260–267.Google Scholar
  17. John, R., P. Ahmad, K. Gadgil, and S. Sharma. 2007. Antioxidative response of Lemna polyrhiza L. to cadmium stress. Journal of Environmental Biology 28 (3): 583–589.Google Scholar
  18. Konar, S.K. 1979. Hazards of water pollution by pesticides. In Proceeding of international symposium held under the joint auspicus of Haryana Agriculture on environmental pollution and toxicology. Nov 28–30: 1977.Google Scholar
  19. Kumari, S., N. Kumar, and S. Ram. 1997. Effects of water pollution on histology of Intestine of two fresh water fishes from Hussainsagar Lake (AP). Indian Journal of Environment and Toxicology 7 (2): 68–70.Google Scholar
  20. Meade, J.W., and R.L. Herman. 1986. Histopathological changes in cultured lake trout, Salvelinus nameycush, subjected to cumulative loading in a water reuse system. Canadian Journal of Fisheries and Aquatic Science 43 (1): 228–231.Google Scholar
  21. Meissner, W.A., and G.T. Diamandopoulos. 1977. Neoplasia, In: Pathology, edited by W.A.D. Anderson, J.M. Kissane (The CV Mosby Co., St. Louis): 640–691.Google Scholar
  22. Monteiro, D.A., J. Alves de Almeida, F.T. Rantin, and A.L. Kalinin. 2006. Oxidative stress biomarkers in the freshwater characid fish, Bryconcephalus, exposed to organophosphate insecticide Folisuper 600 (methyl parathion). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 143 (2): 141–149.Google Scholar
  23. Nagaraju, B., and V.V. Rathnama. 2013. Acute toxicity of chlorantraniliprole to freshwater fish Channa punctatus (Bloch). Advance in Zoology and Botany 1 (4): 78–82.Google Scholar
  24. Nagaraju, B., V.V. Rathnamma, and S. Karra. 2013. Effect of chlorantraniliprole on biochemical and certain biomarkers in various tissues of freshwater fish Labeo rohita (Hamilton). Environment and Ecology Research 1 (4): 205–215.Google Scholar
  25. Oulmi, Y., R.D. Negele, and T. Braunbeck. 1995. Cytopathology of liver and kidney rainbow trout (Oncorhynchus mykiss) aier long-term exposure to sub-lethal concentrations of linuron. Disease of Aquatic Organism 21 (1): 35–52.Google Scholar
  26. Pal, S., E. Kokushi, J. Koyoma, S. Uno, and A.R. Ghosh. 2012. Histopathological alterations in gill, liver and kidney of common carp exposed to chlorpyrifos. Journal of Environmental Science and Health, Part B 47 (3): 180–195.Google Scholar
  27. Rahiminejad, M., S.J. Shahtaheri, M.R. Ganjalim, A. Rahimi Forushani, and F. Golbabaei. 2009. Moleculary imprinted soild phase extraction for trace analysis of diazinon in drinking water. Iranian Journal of Environmental Health Science and Engineering 6 (2): 97–106.Google Scholar
  28. Rahman, M.Z., Z. Hossain, M.F.A. Mollah, and G.U. Ahmed. 2002. Effect of Diazinon 60 EC on Anabas testudineus, Channa punctatus, Barbodes goniontus. Naga, the ICLARM Quarterly 25 (2): 8–12.Google Scholar
  29. Rathnamma, V.V., and B. Nagaraju. 2014a. Oxidative stress induced by chlrorantraniliprole in various tissues of freshwater fish Ctenopharyngodon Idella. Journal of Medical Science and Public Health 2 (1): 21–27.Google Scholar
  30. Rathnamma, V.V., and B. Nagaraju. 2014b. Acute toxicity and Histopathological changes in freshwater fish Cirrhinus mrigala exposed to chlorantraniliprole. The Journal of Zoology Studies 1 (4): 23–30.Google Scholar
  31. Rathnamma, V.V., and B. Nagaraju. 2013. Acute toxicity of chlorantraniliprole to fresh water fish Ctenopharingodon idella (Valenciennes, 1844). Innovare Journal of Life Science 1 (2): 17–20.Google Scholar
  32. Ravindar, K. 2000. Chronic ammonia induced histopathological changes in Indian subtropical freshwater murrel, Channa punctatus (Bloch). Pollution Research 19 (4): 611–613.Google Scholar
  33. Samanta, P., S. Pal, A.K. Mukherjee, T. Senapati, and A.R. Ghosh. 2016. Alterations in digestive enzymes of three freshwater teleostean fishes by Almix herbicide: A comparative study. Procedings of the Zoological Society 69 (1): 61–66.Google Scholar
  34. Sastry, K.V., and S.K. Sharma. 1978. The effect of endrin on the histopathologicl changes in the liver of Channa punctatus. Bulletin of Environmental Contamination and Toxicology 20 (1): 674–677.Google Scholar
  35. Schlenk, D. 2005. Pesticide biotransformation in fish. In Biochemistry and molecular biology of fishes vol. 6, Elsevier B.V., pp. 171–190.Google Scholar
  36. Senapati, T., A.K. Mukherjee, and A.R. Ghosh. 2012. Observations on the effect of Almix 20WP herbicide on ultrastructure (SEM) in different regions of alimentary canal of Anabas testudineus (Cuvier). International Journal of Food, Agriculture Veterinary Science 2 (1): 32–39.Google Scholar
  37. Siang, H.Y., L.M. Yee, and C.T. Seng. 2007. Acute toxicity of organochlorine insecticide endosulfan and its effect on behaviour and some hematological parameters of Asian swamp eel (Monopterus albus, Zuiew). Pesticide Biochemistry and Physiology 89 (1): 46–53.Google Scholar
  38. Stentiford, G.D., M. Longshaw, B.P. Lyons, G. Jones, and M. Green. 2003. Histopathological biomarkers in estuarine fish species for the assessment of biological effects of contaminants. Marine Environmental Research 55 (2): 137–159.Google Scholar
  39. Sweilum, M.A. 2006. Effect of sublethal toxicity of some pesticides on growth parameters, haematological properties and total production of Nile tilapia (Oreochromois niloticus L.) and water quality of ponds. Aquaculture Research 37 (11): 1079–1089.Google Scholar
  40. Szarek, J., A. Siwicki, A. Andrzejewska, E. Terech-Majewska, and T. Banaszkiewicz. 2000. Effects of the herbicide Roundup on the ultrastructural pattern of hepatocytes in carp (Cyprinus carpio). Marine Environmental Research 50 (1–5): 263–266.Google Scholar
  41. Talebi, K. 1998. Diazinon Residues in the Basis of Anzali Lagoon, Iran. Bulletin Environmental Contamination and Toxicology 61 (4): 477–483.Google Scholar
  42. Tarahi Tabrizi, S. 2001. Study of pesticide residues (diazinon, malathion, metasytoux) in the Tabriz Nahand River, Doctoral Dissertation. Tehran University of Medical science, Tehran, Iran.Google Scholar
  43. Triebskorn, R., H.R. Kohler, W. Honnen, M. Schramm, and S.M. Adams. 1997. Induction of heat shock proteins, changes in liver ultrastructure and alterations of fish behaviour: Are these biomarkers related and are they useful to reflect the state of pollution in the field. Journal of Aquatic Ecosystem Stress and Recovery 6 (1): 57–73.Google Scholar
  44. Thurston, R.V., R.C. Russo, R.J. Luedtke, C.E. Smith, E.L. Meyn, C. Chakoumakos, K.C. Wang, and C.J.D. Brown. 1984. Chronic toxicity of ammonia to rainbow trout. Transactions of the American Fisheries Society 113 (1): 56–73.Google Scholar
  45. Venkateswara, J.R. 2006. Toxic effects of novel organophosphorus insecticide (RPR-V) on certain biochemical parameters of euryhaline fish, Oreochromis Mossambicus. Pesticide Biochemistry and Physiology 86 (2): 78–84.Google Scholar

Copyright information

© Zoological Society, Kolkata, India 2019

Authors and Affiliations

  1. 1.Ecotoxicology Laboratory, Department of Environmental ScienceThe University of Burdwan, GolapbagBurdwanIndia
  2. 2.Department of Environmental ScienceAghorekamini Prakashchandra MahavidyalayaHooghlyIndia
  3. 3.P.G. Department of Conservation Biology, Durgapur Government CollegeDurgapurIndia
  4. 4.System Toxicology Research CenterKorea Institute of ToxicologyYuseong-gu, DaejeonRepublic of Korea

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