Effect of cypermethrin on worker and soldier termites of subterranean termites Odontotermes brunneus (Hagen) (Termitidae: Isoptera)
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The termite Odontotermes brunneus is an economically important species causing damage to cellulose containing wooden material and agricultural crops in India. Insecticide application is an effective strategy in termite control. In the present study the effect of cypermethrin was tested for workers and soldiers termite using filter paper dip method. After 24 h treatment the lethal concentration (LC50) was increased to 9.7 ppm in workers and 1.8 ppm in soldiers respectively. The detoxification enzyme activities of esterase, glutathione S-transferase was increased in worker termites 23 µmol, 9 µmol/min/mg of protein compared to soldiers 15 µmol, 7 µmol/min/mg of protein respectively (p < 0.05). The activity of mixed-function oxidase was found very less in both samples. Further nPAGE analysis revealed that increased esterase band in workers than soldier and control sample. The data of this study revealed that possible mechanism of esterase and glutathione S-transferase mediated cypermethrin detoxification that leads to reduce the sensitivity in worker termites of O. brunneus.
KeywordsTerrestrial insect Synthetic pyrethriod Toxicity Detoxification enzymes Electrophoresis
We thank our department of PG and Research Centre in Biotechnology for providing infrastructure facility to carry out this work.
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Conflict of interest
The authors declare that they have no conflict of interest.
- Ahmed, S., and M. Qasim. 2011. Foraging and chemical control of subterranean termites in a farm building at Faisalabad, Pakistan. Pakistan Journal of Life Science 9: 58–62.Google Scholar
- Chottani, O.B. 1997. Fauna of India–isopera (termites), vol. 2, pp. xx + 801 (Published Director. ZSI. Calcutta).Google Scholar
- Dauterman, W.C. 1985. Insect metabolism : Extra microsomal. In Comprehensive insect philology, biochemistry and pharmacology, vol. 12, ed. G.A. Kerkut and L.I. Gilbert, 713–730., Pergamon UK: Oxford.Google Scholar
- Hussain, M.A., 1935. Pest of wheat crop in India. In Proceedings of 2nd world grain exhibition and conference, pp. 562–564.Google Scholar
- Kranthi, K.R. 2005. Insecticides resistance—Monitoring, mechanisms and management manual. Nagpur: CICR.Google Scholar
- Krishna, K., and P.M. Weesner. 1970. In Biology of termites, vol. 2, ed. K. Krishna and F.M. Weesner, 643. New York: Academic Press.Google Scholar
- Kuriachan, I., and R.E. Gold. 1998. Evaluation of the ability of Reticulitermes flavipus Kollar, a subterranean termite (Isoptera: Rhinotermitidae) to differentiate between termiticid treated and untreated soils in laboratory tests. Sociobiology 32: 151–166.Google Scholar
- Osbrink, W.L.A., and A.R. Lax. 2003. Putative resistance to insecticides in the Formosan Subterranean termite an-overview. Sociobiology 41 (1): 143–152.Google Scholar
- Patel, G.A., and H.K. Patel. 1954. Seasonal incidence of termite injury in the northern parts of the Bombay State. Indian Journal of Entomology 15 (4): 376–378.Google Scholar
- Pearce, M.J. 1997. Termites biology and pest management, 172. Cambridge: Cambridge University Press.Google Scholar
- Rajagopal, D. 2002. Economically important termite species in India. Sociobiology 40 (1): 33–46.Google Scholar
- Sattar, A., and Z. Salihah, 2001. Detection and control of subterranean termites. In ed. Technologies for Sustainable Agriculture, Proceedings of national workshop. September 24–26, NIAB, Faisalabad, Pakistan (pp. 195–98).Google Scholar
- Taskin, V., K. Ucukakyuz, T. Arslan, B. Col, and B.G. Taskin. 2007. The biochemical basis of insecticides resistance and determination of esterase enzyme patterns by using PAGE in laboratory collected strains of Drosophila melanogaster from Mulla province of turkey. Journal of Cell and Molecular Biology 6 (1): 31–40.Google Scholar