Polymorphic Microsatellite Markers Selection by Cross-Species Amplification in Black Francolin (Francolinus francolinus asiae)

  • Priyanka NegiEmail author
  • Pramesh Lakhera
Short Communication


The cross-species amplification is an effective method in obtaining microsatellite loci for genetic studies of closely related species. In this study, the cross-species amplified markers were characterized and selected for black francolin (Francolinus francolinus asiae). It is a ground dwelling game bird. In this study total eight markers were cross-species amplified. These eight markers were selected from chicken. The number of alleles per locus ranged from 4 to 21. The observed heterozygosity ranged from 0.022 to 0.98 and the expected heterozygosity from 0.23 to 0.79. There was no evidence of linkage disequilibrium among the pair of loci. These loci were polymorphic and offered valuable tool for genetic diversity and population genetic studies of black francolin.


Black francolin Galliformes Genetic diversity Microsatellite Polymorphism 



The Authors gratefully acknowledge the University Grant Commission (UGC), New Delhi for providing UGC BSR Fellowship (No. F.7-77/2007/BSR) and Department of Biotechnology (DBT), New Delhi for Bioinformatics Centre.


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Copyright information

© Zoological Society, Kolkata, India 2018

Authors and Affiliations

  1. 1.H.N.B, Garhwal UniversitySrinagar GarhwalIndia

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