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Proceedings of the Zoological Society

, Volume 72, Issue 3, pp 283–289 | Cite as

Mosquito Larvicidal Efficacy of Andrographis echioides (Acanthaceae) Foliages Against Vector of Lymphatic Filariasis Culex quinquefasciatus Say (1823)

  • Moumita Das
  • Aniket Singh
  • Kuntal Bhattacharya
  • Ambarish Mukherjee
  • Goutam ChandraEmail author
Research Article
  • 29 Downloads

Abstract

Increasing resistance against synthetic insecticides, mosquito control is becoming serious problem. For an alternative measure novel botanical sources can be used as good insecticides with less toxic hazards to the environment. The present study estimated larvicidal activities of Andrographis echioides against the vector of lymphatic filariasis Culex quinquefasciatus under laboratory conditions. Crude extracts of A. echioides mature leaves were examined for mosquito larvicidal activity against all the larval instars of Cx. quinquefasciatus. Solvent extraction was carried out using three different solvents viz. petroleum ether, ethyl acetate and acetone. Dose dependent larvicidal bioassays were performed using the solvent extractives. LC50 and LC90 values were consummated through log-probit analysis. Regression and ANOVA analyses were done for statistical justification. The effects of crude and solvent extractives were tested on a non-target water fauna. Crude extract of A. echioides leaf exhibited 100% mortality in 1.00% concentration against 1st instars larvae after 72 h of exposure. Among three used solvent extracts ethyl acetate showed efficient larvicidal effect against target mosquito. In ethyl acetate extractive 100.00% mortality was noticed at 150 ppm concentration against 1st instars larvae after 48 h of exposure with LC50 and LC90 values of 32.96 ppm and 106.96 ppm respectively. Tested non target organism was completely safe both from crude and ethyl acetate extractives. This experiment was a pioneer attempt to establish A. echioides as a precious resource for production of mosquito larvicide against Cx. quinquefasciatus.

Keywords

Culex quinquefasciatus Andrographis echioides Larvicidal activities Non-target organism Lymphatic filariasis 

Notes

Acknowledgements

Authors are indebted to UGC for financial support for providing CAS (RTI/1309/184) and Rajiv Gandhi National Fellowship (RGNF-2013-14-ST-WES-52307).

Author Contribution

The screening was done and the plant having mosquitocidal activity was selected by Moumita Das. Rearing of mosquito larvae and colony set up was done by Moumita Das. Extract preparation and laboratory bioassay was carried out by Moumita Das and Aniket Singh. Manuscript preparation was done by Kuntal Bhattacharya. Plant identification was done by Professor Ambarish Mukherjee. Professor Goutam Chandra designed the study and supervised the whole work. All authors read and approved the final version of the manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors have no conflict of interest.

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Copyright information

© Zoological Society, Kolkata, India 2018

Authors and Affiliations

  1. 1.Department of BotanyThe University of BurdwanBurdwanIndia
  2. 2.Mosquito, Microbiology, Nanotechnology Research Units, Parasitology Laboratory, Department of ZoologyThe University of BurdwanGolapbag, BurdwanIndia
  3. 3.Department of ZoologyDurgapur Government CollegeDurgapurIndia

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