Proceedings of the Zoological Society

, Volume 72, Issue 2, pp 180–186 | Cite as

Green Biosynthesis of Silver Nanoparticle Using Garlic, Allium sativum with Reference to Its Antimicrobial Activity Against the Pathogenic Strain of Bacillus sp. and Pseudomonas sp. Infecting Goldfish, Carassius auratus

  • Mandira Saha
  • Probir Kumar BandyopadhyayEmail author
Research Article


Bacterial disease is a growing threat to aquaculture. The use of commercial antibiotics in aquaculture increases antibiotic resistance among pathogenic bacteria in exposed ecosystem and also creating environmental and public health hazards. As a consequence nowadays scientists are in search of alternate antibiotics. The use of Alliums by humans has a long history that can be traced back to the ancient Indians. Therefore, garlic-mediated synthesis of nanoparticles has garnered wide interest owing to its inherent features such as rapidity, simplicity, eco-friendliness and cheaper costs. This study deals with an environmentally friendly and biosynthesis process of antibacterial silver nanoparticles using an aqueous extract of Allium sativum. The formation and characterization of AgNPs was confirmed by scanning electron microscope and X-ray diffraction profile. Furthermore, the synthesized nanoparticles were examined against the pathogenic bacteria such as Pseudomonas aeruginosa PB112 (JN996498) and Bacillus licheniformis PKBMS16 (KX235179) infecting goldfish, Carassius auratus. From the experiment, it was found that the biologically synthesized nanoparticles by using A. sativum have a significant antibacterial activity and provide a scope for possible development of the formulations antimicrobial drug.

Graphical Abstract


Allium sativum Green synthesis Silver nanoparticles SEM analysis XRD analysis Antibacterial activity 



Authors are thankful to the Department of Zoology, University of Kalyani, Kalyani for providing the laboratory facilities. Sincere thanks are also due to the Centre for research of Nanoscience and Nanotechnology, University of Calcutta, Salt Lake City, Kolkata-91 for technical support during SEM study of the parasites.


One of the authors (MS) is thankful to the University Grants Commission, New Delhi for financial support under Special Assistance Program No. F-3-11/2012(SAP-II).

Compliance with Ethical Standards

Conflict of interest

The authors declared that they have no conflicts of interest.

Ethical Approval

The fishes were collected form different ornamental fish farms of West Bengal.


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Copyright information

© Zoological Society, Kolkata, India 2017

Authors and Affiliations

  1. 1.Parasitology Laboratory, Department of ZoologyUniversity of KalyaniKalyaniIndia

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