Determination of Chronic Median Lethal Concentration of Sodium Fluoride in Drosophila melanogaster and Exploring Effect of Sub-lethal Concentrations on Differential Hemocyte Count
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The prerequisite for toxicity assessment of any chemical is the determination of its median lethal concentrations and thus, this study initiates with determination of chronic median lethal concentration of sodium fluoride (NaF) in a non-target organism Drosophila melanogaster, which was found to be 125–130 µg/mL. Further, chronic exposure to NaF at sub-lethal concentrations (10–100 µg/mL) resulted in significant reduction in total hemocyte number in general and plasmatocytes in particular, whereas, crystal cells were significantly increased in number. Since hemocytes are an integral part of innate immune system in Drosophila, their numerical fluctuation confirms fluoride-induced compromise in innate immunity status of Drosophila.
KeywordsFluoride Fruit fly LC50 Trypan blue
The authors are grateful to the Head, DST-FIST, DST-PURSE and UGC-DRS sponsored Department of Zoology, the University of Burdwan for providing the infrastructural facilities. Thanks are due to Dr. A. Mazumdar for kindly providing microscopic facilities.
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