Mild foot electrical stimulation is comparable with phenytoin in inhibiting pentylenetetrazol-induced kindling in rats

  • Arefe Ghasemi-Dehno
  • Abolfazl Jand
  • Monir Abasi-Moghadam
  • Mehdi Sadegh
  • Morteza Mousavi-Hasanzadeh
  • Mohammad Reza PalizvanEmail author
Original Paper


Increasing evidence demonstrates that electric stimulation has anticonvulsant effects. The present study was undertaken to investigate the effects of mild foot electrical stimulation (MFES) on the development of pentylenetetrazol (PTZ) kindling and compare its effectiveness with the more commonly used treatment, phenytoin. Kindling was induced in rats by repeated injections (every 24 h) of PTZ (37.5 mg/kg). The rats were subjected to either MFES (0.2 mA in intensity for a 160 ms duration with a 160 ms interval for 20 min) or phenytoin (30 mg/kg) before PTZ injections. Following this treatment, rats received MFES every other day for 10 days or 26 days after establishment of PTZ kindling. The data showed that MFES significantly inhibited development of chemical kindling induced by PTZ in rats (p = 0.001, as compared to PTZ-treated animals). This inhibitory effect is comparable with the effect of 30 mg/kg doses of phenytoin (P = 0.99, as compared to phenytoin group). However, 10 days or 26 days durations of MFES had no effect on established kindled seizures (P = 0.58 as compared to PTZ-treated animals). Our data demonstrate that although MFES significantly inhibited the development of chemical kindling, this experimental paradigm had no effect on established kindled seizures.


Pentylenetetrazol Kindling Phenytoin Electrical stimulation Epilepsy 



Financial support for this study was provided by Deputy Vice-chancellor of research on Arak University of Medical Sciences Grant # 2283.

Compliance with ethical standards

Ethical approval

Ethical approval for the study was provided by the Arak University of Medical Sciences Research Ethics Committee # 94-132.

Conflict of interest

The authors declare no conflict of interest.


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Copyright information

© The Physiological Society of Japan and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Physiology, Faculty of MedicineArak University of Medical SciencesSardashtIran

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