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The Journal of Physiological Sciences

, Volume 69, Issue 1, pp 143–149 | Cite as

MARCKS phosphorylation and amylase release in GLP-1-stimulated acini isolated from rat pancreas

  • Keitaro SatohEmail author
  • Motoshi Ouchi
  • Asuka Morita
  • Masanori Kashimata
Short Communication

Abstract

Little is known about the effects of glucagon-like peptide 1 (GLP-1) on the pancreatic exocrine gland. In the gland, secretagogues induce amylase release. That signal transduction is evoked mainly by an increase in intracellular Ca2+ levels and activation of protein kinase C (PKC). We previously demonstrated that myristoylated alanine-rich C kinase substrate (MARCKS), a PKC substrate, is involved in pancreatic amylase release. Here, we studied the effects of GLP-1 on MARCKS phosphorylation and amylase release in rat pancreatic acini. GLP-1 induced amylase release and MARCKS phosphorylation in isolated pancreatic acini. Inhibitors of cAMP-dependent protein kinase (PKA) suppressed those effects. Furthermore, a MARCKS-related peptide inhibited the GLP-1-induced amylase release. These findings suggest that GLP-1 induces amylase release through MARCKS phosphorylation via activation of PKA in isolated pancreatic acini.

Keywords

GLP-1 MARCKS Amylase PKA Exocytosis 

Notes

Acknowledgements

The authors thank Dr. Noriko Koyama (Asahi University School of Dentistry) for helpful advice.

Author contributions

KS conception and design of research; KS, MO, and AM performed experiments; KS, MO, and AM analyzed data; KS, MO, and MK interpreted results of experiments; KS prepared figures; KS drafted manuscript; KS, MO, AM, and MK edited and revised the manuscript; KS, MO, AM, and MK approved final version of manuscript.

Funding

This work was supported by JSPS KAKENHI Grant numbers 15K21322 and 15K11060.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

In accordance with the established related guidelines, the experimental design of the study was approved by the Animal Offices of Asahi University (Approval number: 17-024) and Dokkyo Medical University (Approval numbers: 0876 and 1046).

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Copyright information

© The Physiological Society of Japan and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Keitaro Satoh
    • 1
    Email author
  • Motoshi Ouchi
    • 2
  • Asuka Morita
    • 2
  • Masanori Kashimata
    • 1
  1. 1.Department of PharmacologyAsahi University School of DentistryMizuhoJapan
  2. 2.Department of Pharmacology and ToxicologyDokkyo Medical University School of MedicineMibuJapan

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