A rotating cerium anode X-ray system allows visualization of intramural coronary vessels after cardiac stem cell therapy for myocardial infarction
- 162 Downloads
Conventional angiography is insufficient for evaluating the therapeutic effect of cardiac regeneration therapy. A microangiographic X-ray system using a cerium anode was developed. Cerium has a characteristic X-ray with a peak at 34.6 keV, which allows visualization of tiny amounts of iodine. The performance of the cerium anode X-ray system was evaluated in two excised normal canine hearts and in excised ischemic canine hearts treated with c-kit-positive cardiac stem cells (5 canines) or without cells (5 control canines). In the normal canines, branches penetrating from the left anterior descending artery into the myocardium were visualized, down to third-order branches. In just the treated hearts treated with stem cells, small vessels characterized by irregular vessel walls were observed. The cerium anode X-ray system allowed visualization of microvessels in excised ischemic canine hearts, and may evaluate the effect of cardiac stem cell therapy.
KeywordsMicroangiography C-kit-positive cardiac stem cells Cerium anode Myocardial infarction Canine models
The study was supported by Grants-in-Aid for Scientific Research B (20390336, 2007–2010), Grant-in-Aid for Young Scientists B (25861233, 2013–2014), and Grants-in-Aid from The Cardiovascular Research Fund, Tokyo, Japan (H24-06-01, 2013–2014). Additionally, it was partly supported by a research grant from Health and Labour Sciences Research Grant (200624005A, 2004–2006) and The Research Funding for Longevity Sciences from National Center for Geriatrics and Gerontology (NCGG), Japan (22-5, 2010–2012). We would like to acknowledge Masaki Inoue, Emiko Hayashi (Tokai University Institute of Innovative Science and Technology, Kanagawa, Japan), Sachie Tanaka, Yoko Takahari, Yoshiko Shinozaki, Katsuko Naito (Support Center for Medical Research and Education, Tokai University School of Medicine, Kanagawa, Japan), Fujio Ando and Rie Hasegawa (Tokai University Imaging Center, Kanagawa, Japan), who contributed to the experiments.
Design of study; CT, TH, HM. Experiment; CT, TH, YI, YS, KT, TS, TS. NF, HM. Data collecting and analyzing; CT, KT, HM, Drafting the article; CT, TH, HM.
Compliance with ethical standards
This study was funded by Grants-in-Aid for Scientific Research B (20390336, 2007–2010), Grant-in-Aid for Young Scientists B (25861233, 2013–2014), and Grants-in-Aid from The Cardiovascular Research Fund, Tokyo, Japan (H24-06-01, 2013–2014). Additionally, it was partly supported by a research grant from Health and Labour Sciences Research Grant (200624005A, 2004–2006) and The Research Funding for Longevity Sciences from National Center for Geriatrics and Gerontology (NCGG), Japan (22-5, 2010–2012).
Conflict of interest
The authors declare that they have no conflict interest other than the funding mentioned above.
Research involving animals
All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.
This article does not contain any studies with human participants.
- 5.Rosalinda M, Linda WVL, Sean MD, Felix BE, Derek JH, Sandrine L, Jonathan L, Cinzia P, Rainer S, Kirsti Y, Ulf L, Christine LM, Stefan J, James W, Thomas E, Péter F, Joost PGS (2016) Position paper of the european society of cardiology working group cellular biology of the heart: cell-based therapies for myocardial repair and regeneration in ischemic heart disease and heart failure. Eur Heart J. doi: 10.1093/eurheartj/ehw113 CrossRefGoogle Scholar
- 9.Bearzi C, Rota M, Hosoda T, Tillmanns J, Nascimbene A, De Angelis A, Yasuzawa-Amano S, Trofimova I, Siggins RW, Lecapitaine N, Cascapera S, Beltrami AP, D’Alessandro DA, Zias E, Quaini F, Urbanek K, Michler RE, Bolli R, Kajstura J, Leri A, Anversa P (2007) Human cardiac stem cells. Proc Natl Acad Sci USA 104:14068–14073CrossRefGoogle Scholar
- 10.Bearzi C, Leri A, Lo Monaco F, Rota M, Gonzalez A, Hosoda T, Pepe M, Qanud K, Ojaimi C, Bardelli S, D’Amario D, D’Alessandro DA, Michler RE, Dimmeler S, Zeiher AM, Urbanek K, Hintze TH, Kajstura J, Anversa P (2009) Identification of a coronary vascular progenitor cell in the human heart. Proc Natl Acad Sci USA 106:15885–15890CrossRefGoogle Scholar
- 11.Bolli R, Chugh AR, D’Amario D, Loughran JH, Stoddard MF, Ikram S, Beache GM, Wagner SG, Leri A, Hosoda T, Sanada F, Elmore JB, Goichberg P, Cappetta D, Solankhi NK, Fahsah I, Rokosh DG, Slaughter MS, Kajstura J, Anversa P (2011) Cardiac stem cells in patients with ischaemic cardiomyopathy (scipio): initial results of a randomised phase 1 trial. Lancet 378:1847–1857CrossRefGoogle Scholar
- 12.Chugh AR, Beache GM, Loughran JH, Mewton N, Elmore JB, Kajstura J, Pappas P, Tatooles A, Stoddard MF, Lima JA, Slaughter MS, Anversa P, Bolli R (2012) Administration of cardiac stem cells in patients with ischemic cardiomyopathy: the scipio trial: surgical aspects and interim analysis of myocardial function and viability by magnetic resonance. Circulation 126:S54–S64CrossRefGoogle Scholar
- 13.Mori H, Hyodo K, Tanaka E, Uddin-Mohammed M, Yamakawa A, Shinozaki Y, Nakazawa H, Tanaka Y, Sekka T, Iwata Y, Handa S, Umetani K, Ueki H, Yokoyama T, Tanioka K, Kubota M, Hosaka H, Ishikawa N, Ando M (1996) Small-vessel radiography in situ with monochromatic synchrotron radiation. Radiology 201:173–177CrossRefGoogle Scholar
- 15.Abudurexiti A, Kameda M, Sato E, Abderyim P, Enomoto T, Watanabe M, Hitomi K, Tanaka E, Mori H, Kawai T, Takahashi K, Sato S, Ogawa A, Onagawa J (2010) Demonstration of iodine k-edge imaging by use of an energy-discrimination x-ray computed tomography system with a cadmium telluride detector. Radiol Phys Technol 3:127–135CrossRefGoogle Scholar
- 21.Tanaka A, Mori H, Tanaka E, Mohammed MU, Tanaka Y, Sekka T, Ito K, Shinozaki Y, Hyodo K, Ando M, Umetani K, Tanioka K, Kubota M, Abe S, Handa S, Nakazawa H (1999) Branching patterns of intramural coronary vessels determined by microangiography using synchrotronradiation. Am J Physiol 276(6 Pt 2):H2262–H2267PubMedGoogle Scholar