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The Journal of Physiological Sciences

, Volume 68, Issue 1, pp 43–53 | Cite as

Branched-chain amino acids administration suppresses endurance exercise-related activation of ubiquitin proteasome signaling in trained human skeletal muscle

  • Evgeny A. LysenkoEmail author
  • Tatiana F. Vepkhvadze
  • Egor M. Lednev
  • Olga L. Vinogradova
  • Daniil V. Popov
Original Paper

Abstract

We tested whether post exercise ingestion of branched-chain amino acids (BCAA < 10 g) is sufficient to activate signaling associated with muscle protein synthesis and suppress exercise-induced activation of mechanisms associated with proteolysis in endurance-trained human skeletal muscle. Nine endurance-trained athletes performed a cycling bout with and without BCAA ingestion (0.1 g/kg). Post exercise ACCSer79/222 phosphorylation (endogenous marker of AMPK activity) was increased (~3-fold, P < 0.05) in both sessions. No changes were observed in IGF1 mRNA isoform expression or phosphorylation of the key anabolic markers — p70S6K1Thr389 and eEF2Thr56 — between the sessions. BCAA administration suppressed exercise-induced expression of mTORC1 inhibitor DDIT4 mRNA, eliminated activation of the ubiquitin proteasome system, detected in the control session as decreased FOXO1Ser256 phosphorylation (0.83-fold change, P < 0.05) and increased TRIM63 (MURF1) expression (2.4-fold, P < 0.05). Therefore, in endurance-trained human skeletal muscle, post exercise BCAA ingestion partially suppresses exercise-induced expression of PGC-1a mRNA, activation of ubiquitin proteasome signaling, and suppresses DDIT4 mRNA expression.

Keywords

Endurance exercise Branched-chain amino acids Proteolysis Mitochondrial biogenesis 

Notes

Acknowledgements

The authors are grateful to Arvo Leinsoo and Anton Bachinin for technical support and Dr. Dmitry Perfilov for tissue acquisition. This study was funded by the Russian Science Foundation (grant no. 14-15-00768, control experiment) and the Russian Foundation for Basic Research (Grant No. 14-04-01807a, BCAA experiment).

Author contributions

The study was designed by DVP, EAL and OLV. Data were collected and analyzed by EAL, TFV, EML and DVP. Data interpretation and manuscript preparation were undertaken by EAL, OLV, and DVP. All authors approved the final version of the paper.

Compliance with ethical standards

Conflict of interest

Evgeny A. Lysenko declares that he has no conflict of interest. Tatiana F. Vepkhvadze declares that she has no conflict of interest. Egor M. Lednev declares that he has no conflict of interest. Olga L. Vinogradova declares that she has no conflict of interest. Daniil V. Popov declares that he has no conflict of interest.

This study was funded by the Russian Science Foundation (Grant no. 14-15-00768, control experiment) and the Russian Foundation for Basic Research (Grant no. 14-04-01807a, BCAA experiment).

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© The Physiological Society of Japan and Springer Japan 2016

Authors and Affiliations

  1. 1.Laboratory of Exercise Physiology, Institute of Biomedical ProblemsRussian Academy of SciencesMoscowRussia
  2. 2.Faculty of Fundamental MedicineM.V. Lomonosov Moscow State UniversityMoscowRussia

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