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Anatomical Science International

, Volume 94, Issue 1, pp 86–100 | Cite as

Distribution of somatostatin-28 (1-12), calcitonin gene-related peptide, and substance P in the squirrel monkey brainstem: an immunocytochemical study

  • E. Duque-DíazEmail author
  • R. Coveñas
Original Article
  • 32 Downloads

Abstract

Using an immunocytochemical technique, we have studied the distribution of fibers and cell bodies containing somatostatin-28 (1-12) [SOM-28 (1-12)], calcitonin gene-related peptide (CGRP), and substance P (SP) in the brainstem of Saimiri sciureus. The distribution of the peptidergic cell bodies was very restricted: perikarya containing SOM-28 (1-12) were only observed in the substantia grisea centralis, while no immunoreactive cell bodies containing CGRP or SP were visualized. Fibers containing SOM-28 (1-12), CGRP, or SP were widely distributed in the brainstem: immunoreactive fibers containing SOM-28 (1-12) showed the most widespread distribution and were the most abundant. The distribution of SOM-28 (1-12)-, CGRP- or SP-immunoreactive fibers was very similar. Colocalization of immunoreactive fibers containing SOM-28 (1-12), CGRP or SP was observed in many brainstem nuclei. A neuroanatomical relationship between CGRP- and SP-immunoreactive fibers was observed, although this relationship was less marked for SOM-28 (1-12) and SP and lower still for SOM-28 (1-12) and CGRP. The widespread distribution of the peptidergic fibers suggests that the studied neuropeptides are involved in many physiological actions.

Keywords

SOM-28 (1-12) CGRP SP Mesencephalon Pons Medulla oblongata Saimiri sciureus 

Abbreviations

Ann

Nucleus annularis

AS

Aquaeductus Sylvii

BC

Brachium conjunctivum

BP

Brachium pontis

Cd

Nucleus caudatus

CD

Nucleus cochlearis dorsalis

Cn

Nucleus cuneatus

CnL

Nucleus cuneatus lateralis

CM

Centrum medianum thalami

CoS

Colliculus superior

CT

Corpus trapezoideum

CV

Nucleus cochlearis ventralis

DBC

Decussatio brachii conjunctivi

DG

Nucleus dorsalis tegmenti (Gudden)

DPy

Decussatio pyramidum

DR

Nucleus dorsalis raphae

DV

Nucleus dorsalis nervi vagi

FC

Funiculus gracilis

FRM

Formatio reticularis myelencephali

FRPo

Formatio reticularis pontis oralis

FRTM

Formatio reticularis tegmenti mesencephali

G

Nucleus gracilis

GC

Substantia grisea centralis

GCv

Substantia grisea centralis, pars ventralis

GL

Corpus geniculatum lateralis

GM

Corpus geniculatum medialis

GPo

Griseum pontis

IP

Nucleus interpeduncularis

LC

Locus coeruleus

LM

Nucleus lemnisci lateralis

LP

Nucleus lateralis posterior thalami

MD

Nucleus medialis dorsalis thalami

NCI

Nucleus colliculi inferioris

NCS

Nucleus centralis superior tegmenti

NIII

Nucleus nervi oculomotorii

nIV

Nervus trochlearis

NIV

Nucleus nervi trochlearis

NR

Nucleus ruber

NSv

Nucleus tractus spinalis nervi trigemini

NTS

Nucleus tractus solitarii

nV

Nervus trigemini

NVI

Nucleus nervi abducentis

NVII

Nucleus nervi facialis

nVIIIa

Nervus acusticus

NXII

Nucleus nervi hypoglossi

OI

Nucleus olivaris inferior

OIm

Nucleus olivaris inferior, accessorius medialis

OIp

Nucleus olivaris inferior principalis

OS

Nucleus olivaris superior

PbL

Nucleus parabrachialis lateralis

PbM

Nucleus parabrachialis medialis

pf

Nucleus parafascicularis thalami

Pp

Nucleus praepositus

PTc

Nucleus praetectalis

PuO

Nucleus peripeduncularis oralis thalami

Pv

Nucleus principalis n. trigemini

Py

Tractus pyramidalis

RL

Nucleus reticularis lateralis myelencephali

RPm

Nucleus reticularis paramedianus myelencephali

RTP

Nucleus reticularis tegmenti pontis

SNc

Substantia nigra, pars compacta

SNd

Substantia nigra, pars diffusa

TSc

Tractus tectospinalis nervi trigemini

TTS

Tractus tectospinalis

VesI

Nucleus vestibularis inferior

VesL

Nucleus vestibularis lateralis

VesM

Nucleus vestibularis medialis

VesS

Nucleus vestibularis superior

Notes

Acknowledgements

This work has been supported by the Universidad de Santander UDES, Bucaramanga (Colombia). The authors wish to thank Professor Gérard Tramu (Université de Bordeaux I, France) for the gift of primary antibodies, and the language service of the Universidad de Santander UDES (Colombia) for supervising the English text. This work is dedicated to Professor Julio Villalobos (Universidad del Valle, Cali, Colombia).

Compliance with ethical standards

Conflict of interest

None.

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Copyright information

© Japanese Association of Anatomists 2018

Authors and Affiliations

  1. 1.Universidad de Santander UDES, School of Medicine, Neuroscience GroupBucaramangaColombia
  2. 2.Institute of Neuroscience of Castilla y León (INCYL), Laboratory of Neuroanatomy of the Peptidergic Systems (Lab. 14)University of SalamancaSalamancaSpain

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