Anatomical Science International

, Volume 94, Issue 2, pp 192–198 | Cite as

Ontogenic development of the digestive tract in larval and juvenile Vimba bream, Vimba vimba

  • Sara Jalali
  • Shahla JamiliEmail author
  • Mohammad Sayyad Bourani
  • Ehsan Ramezani-Fard
  • Abolfazl Sepahdari
Original Article


In this study we examined the ontogenic development of the digestive tract of Vimba bream (Vimba vimba, Family: Cyprinidae) during the first 60 days of life (hatching to 60 days after hatching [DAH]). Samples of developing Vimba bream were randomly selected at various stages of development: 1–8, 10, 15, 20, 25, 30, 40, 50, and 60 DAH. For the histological and histochemical studies on the development of the alimentary canal, tissue sections prepared from the sampled hatchlings were stained with hematoxylin–eosin and periodic acid–Schiff and observed under a light microscope. The histological structures of both the mouth and esophagus were fully developed at 5 and 7 DAH, respectively. Intestinal differentiation was observed at 2 DAH, while mucosal folds appeared on the intestinal bulb at 7 DAH. At 5 DAH, with the appearance of goblet cells in the epithelium of the mouth, pharynx, and esophagus, the larvae showed secretion activity in these organs. At 6 DAH, secretion was observed in the intestine; at this stage of development, the surface of the gastrointestinal tract was covered in a neutral mucous-like layer of polysaccharide. The histological observations indicate that the early development of the digestive tract in Vimba vimba enables larvae to efficiently ingest and digest exogenous feed very quickly after hatching.


Development Digestive tract Histochemistry Ontogeny Vimba bream 



The authors would like to thank the head and the staff of Shahid Ansari Hatchery and Aquaculture Center in Rasht, Iran for their cooperation and assistance and Dr. Douglas Rideout at Université de Montreal, Montreal, QC and Mrs. Sheryl Nikpoor for proof-reading the text and their invaluable editorial comments on the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Abdoli A, Naderi M (2009) Biodiversity of fishes of the southern basin of the Caspian sea. Abzian Scientific Publication, Tehran (in Farsi)Google Scholar
  2. Baglole CJ, Murrary HM, Goff GP, Wright GM (1997) Ontogeny of the digestive tract during larval development of yellowtail flounder: a light microscopic and mucous histochemical study. J Fish Biol. Google Scholar
  3. Boulhic M, Gabaudan J (1992) Histological study of the organogenesis of the digestive system and swim bladder of the Dover Sole, Solea solea (Linnaeus 1758). Aquaculture. Google Scholar
  4. Canino MF, Bailey KM (1995) Gut evacuation of Walleye pollock larvae in response to feeding conditions. J Fish Biol 46(3):389–403CrossRefGoogle Scholar
  5. Dettlaf TA, Ginsburg AS, Schmal Hasen OI (1993) Sturgeon fishes: developmental biology and aquaculture. Springer, BerlinCrossRefGoogle Scholar
  6. Dunaevskaya E (2010) Histological investigation of organs and tissues development of Ballan wrasse larvae during ontogenesis. MSc thesis. Bodø University College, BodøGoogle Scholar
  7. Elbal MT, Garcia Hernandez MP, Lozano MT, Agulleiro B (2004) Development of the digestive tract of gilthead sea bream (Sparus aurata L.). Light and electron microscopic studies. Aquaculture. Google Scholar
  8. Gauthier GF, Landis SC (1972) The relationship of ultrastructural and cytochemical features to absorptive activity in the goldfish intestine. Anat Rec 172:675–701CrossRefGoogle Scholar
  9. Gawlicka A, Teh SJ, Hung SSO, Hinton DE, De lanoue J (1995) Histological and histochemical changes in the digestive tract of white sturgeon larvae during ontogeny. J Fish Physiol Biochem 14:357–371CrossRefGoogle Scholar
  10. Gordon AK, Hecht T (2002) Histological studies on the development of the digestive system of the clownfish Amphiprion percula and the time of weaning. J Appl Ichthyol. Google Scholar
  11. Guerrera MC, De Pasquale F, Muglia U, Caruso G (2015) Digestive enzymatic activity during ontogenetic development in Zebrafish (Danio rerio). J Exp Zool (Mol Dev Evol). Google Scholar
  12. Hachero-Cruzado I, Ortiz-Delgado JB, Borrega B, Herrera M, Navas JI, Sarasquete C (2009) Larval organogenesis of flatfish brill Scophthalmus rhombus L: Histological and histochemical aspects. Aquaculture. Google Scholar
  13. Khoshnood Z, Khodabandeh S, Shahryari Moghaddam M, Mosafer Khorjestan S (2011) Histopathological and pathomorphological effects of mercuric chloride on the gills of Persian Sturgeon (Acipenser persicus) Fry. IJNRMS (ECOPERSIA) 1(1):23–32Google Scholar
  14. Kozaric Z, Kuzir S, Petrinec Z, Gjurcevic E, Bozic M (2008) The development of the digestive tract in larval European catfish (Silurus glanis L.). J Anat Histol Embryol. Google Scholar
  15. Mai K, Yu H, Ma H, Duan Q, Gisbert E, Infante JLZ, Cahu CL (2005) A histological study on the development of the digestive system of Pseudosciaena crocea larvae and juveniles. J Fish Biol 67:1094–1106CrossRefGoogle Scholar
  16. Moshayedi F (2016) Growth pattern and ontogeny of digestive system in the common carp (Cyprinus carpio) (Var. Sazan) during larval development. MSc thesis. University of Tehran, TehranGoogle Scholar
  17. Neuhaus H, Van Der Marel M, Caspari N, Meyer W, Enss ML, Steinhagen D (2007) Biochemical and histological study on the intestinal mucosa of the common carp Cyprinus carpio L., with special consideration of mucin glycoproteins. J Fish Biol. Google Scholar
  18. Noaillac-Depeyre J, Gas N (1976) Electron microscopic study on gut epithelium of the tench (Tinca tinca L.) with respect to its absorptive functions. Tissue Cell 8:511–530CrossRefGoogle Scholar
  19. Ostasewska T (2005) Developmental changes of digestive system structures in Pike-Perch (Sander lucioperca L.). Electron J Ichthyol 2:65–78Google Scholar
  20. Pearse AG (1985) Histochemistry, theoretical and applied, analytical technology. Churchill Livingstone, LondonGoogle Scholar
  21. Purushothaman K, Lau D, Saju JM, Syed Musthaqsk SK, Lunny DP, Vij S, Orban L (2016) Morpho-histological characterization of the alimentary canal of an important food fish, Asian sea bass (Lates calcarifer). PeerJ 4:e2377. CrossRefGoogle Scholar
  22. Ramezani-Fard E, Kamarudin MS, Harmin AS, Saad CR, Abd Sattar MK, Daud SK (2011) Ontogenic development of the mouth and digestive tract in larval Malaysian mahseer, Tor tambroides Bleeker. J Appl Ichthyol. Google Scholar
  23. Sahlmann C, Gu J, Kartner TM, Lein I, Krogdahl A, Bakke AM (2015) Ontogeny of the digestive system of Atlantic Salmon (Salmo salar L.) and effects of soybean meal from start feeding. Plos One 10(4):e0124179. CrossRefGoogle Scholar
  24. Sarasquete MC, Polo A, Yufera M (1995) Histology and histochemistry of the development of the digestive system of larval gilthead sea bream, Sparus aurata L. Aquaculture. Google Scholar
  25. Shahriari Moghadam M, Abtahi B, Rezaei S, Rahdari A (2014) Early ontogenic development of digestive system in Schizothorax zarudnyi Nikolskii, 1897 (Actinopterygii: Cyprinidae) larvae. Ital J Zool. Google Scholar
  26. Stroband HWJ, Meer HVD, Timmermans LPM (1979) Regional functional differentiation in the gut of the Grasscarp, Ctenopharyngodon idella (Val.). Histochemistry 64:235–249CrossRefGoogle Scholar
  27. Unal G, Centinkaya O, Kankaya E, Elp M (2001) Histological study of the organogenesis of the digestive system and swim bladder of the Calchalburnus tarichi pallas, 1811 (Cyprinidae). Turk J Zool 25:217–228Google Scholar
  28. Yaghobi M (2012) Histological development of the alimentary channel of Caspian roach (Rutilus rutilus caspius) from hatching to fingerling size. MSc thesis. University of Tehran, TehranGoogle Scholar
  29. Zambonino Infante JL, Gisbert E, Sarasquete C, Navarro I, Gutierrez J, Cahu C (2008) Ontogeny and physiology of the digestive system of marine fish larvae. In: Cyrino JEP, Byreau D, Kapoor BG (eds) Feeding and digestive functions in fishes. Science Publishers, Enfield, pp 281–348CrossRefGoogle Scholar

Copyright information

© Japanese Association of Anatomists 2019

Authors and Affiliations

  • Sara Jalali
    • 1
  • Shahla Jamili
    • 3
    Email author
  • Mohammad Sayyad Bourani
    • 2
  • Ehsan Ramezani-Fard
    • 1
  • Abolfazl Sepahdari
    • 3
  1. 1.Department of Natural Resources and Environment, Science and Research BranchIslamic Azad UniversityTehranIran
  2. 2.Inland Water Aquaculture Research Center, Iranian Fisheries Sciences Research InstituteAgricultural Research Education and Extension Organization (AREEO)AnzaliIran
  3. 3.Iranian Fisheries Sciences Research InstituteAgricultural Research Education and Extension Organization (AREEO)TehranIran

Personalised recommendations