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Relationship between abundance and localization of maternal messenger RNA and egg quality in artificially matured Japanese eel Anguilla japonica

  • Moemi Horiuchi
  • Hikari Izumi
  • P. Mark Lokman
  • Shigeho IjiriEmail author
  • Shinji Adachi
Original Article Biology

Abstract

Artificial maturation and ovulation of Japanese eel do not always result in the production of good-quality eggs. The molecular basis for compromised egg quality in artificially matured Japanese eel remains unknown. In this study, we investigated the relationship between the localization of six maternal transcripts, grip2, dazl, sybu, trim36, pou5f3 and npm2, and egg quality in artificially matured Japanese eel oocytes and eggs. We observed that localization of these messenger RNAs (mRNAs) in oocytes from the perinucleolus stage to the migratory nucleus stage is not associated with egg quality. In contrast, in ovulated eggs, we observed two different mRNA localization patterns, which we designated type A (mRNA localized in the cortex) and type B (mRNA distributed more centrally). Furthermore, individuals containing many type B eggs tended to have low fertilization and hatching rates, but this was not reflected in the target gene mRNA levels. Our results suggest that the loss of quality in eggs from artificially matured Japanese eel is caused by abnormal mRNA localization even if there is no difference in mRNA levels.

Keywords

Maternal transcript Egg quality Japanese eel Localization 

Notes

Acknowledgements

We thank the team members of the Laboratory of Fish Reproductive Physiology at the Graduate School of Fisheries Sciences, Hokkaido University for their assistance and helpful advice. This study was partly supported by Japan Society for the Promotion of Science KAKENHI grant no. 26252030.

Supplementary material

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Supplementary material 1 (DOCX 76 kb)
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Supplementary material 4 (DOCX 926 kb)

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Copyright information

© Japanese Society of Fisheries Science 2019

Authors and Affiliations

  • Moemi Horiuchi
    • 1
  • Hikari Izumi
    • 1
  • P. Mark Lokman
    • 2
  • Shigeho Ijiri
    • 1
    Email author
  • Shinji Adachi
    • 1
  1. 1.Graduate School of Fisheries SciencesHokkaido UniversityHakodateJapan
  2. 2.Department of ZoologyUniversity of OtagoDunedinNew Zealand

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