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Fisheries Science

, Volume 85, Issue 6, pp 1011–1018 | Cite as

Antagonistic activity and protective effect of a Bacillus subtilis isolate against fish pathogen Edwardsiella piscicida

  • Xiaomeng Ren
  • Binbin Wu
  • Feng Zhao
  • Lingling Qi
  • Xianfeng Qiu
  • Ran Li
  • Sisi Yang
  • Fuguo Liu
  • Ganfeng Yi
  • Xuezhi Ding
  • Liqiu Xia
  • Yunjun SunEmail author
Original Article Aquaculture

Abstract

Bacillus subtilis ZFB19 isolated from the intestine of healthy grass carp exhibited considerable antagonistic activity against Edwardsiella piscicida which was resistant to various antibiotics. Its extracellular antibacterial substance was stable under wide range of pH, temperature, and proteinases. The active component was purified through HPLC and exhibited molecular weight of 560.28198 as revealed by mass spectrometric analysis. By utilizing adult zebrafish as well as ZF4 cell line, we found that pretreatment of zebrafish and ZF4 cell with B. subtilis ZFB19 could attenuate E. piscicida-induced mortality. The lymphoid tissues (liver, spleen, and kidney) of zebrafish injected with strain ZFB19 showed significant enhancement in the levels of respiratory burst activity, nitric oxide production, lysozyme activity and total protein content. Meanwhile, initial internalized E. piscicida and its intracellular replication in ZF4 cells could be obviously reduced by B. subtilis pretreatment. This study is helpful for understanding the mechanism that underlies the effects of probiotic Bacillus in reducing fish pathogen infection.

Keywords

Antagonistic activity Bacillus subtilis Edwardsiella piscicida Protective effect Zebrafish 

Notes

Acknowledgements

We thank Prof. Yuanxing Zhang for providing Edwardsiella piscicida strain EIB202. This work was supported by Scientific Research Fund of Hunan Provincial Education Department (18K023), Graduate Teaching Reform Program of Hunan Normal University (12JG14), and the Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (20134486), P. R. China.

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Copyright information

© Japanese Society of Fisheries Science 2019

Authors and Affiliations

  • Xiaomeng Ren
    • 1
  • Binbin Wu
    • 1
  • Feng Zhao
    • 1
  • Lingling Qi
    • 1
  • Xianfeng Qiu
    • 1
  • Ran Li
    • 1
  • Sisi Yang
    • 1
  • Fuguo Liu
    • 1
  • Ganfeng Yi
    • 1
  • Xuezhi Ding
    • 1
  • Liqiu Xia
    • 1
  • Yunjun Sun
    • 1
    Email author
  1. 1.State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life ScienceHunan Normal UniversityChangshaPeople’s Republic of China

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