Antagonistic activity of marine Streptomyces sp. S073 on pathogenic Vibrio parahaemolyticus
Marine Streptomyces sp. isolate S073 was shown to have strong antagonistic activity towards the pathogenic Vibrio parahaemolyticus using the agar diffusion method. The antagonistic substance(s) secreted into the supernatant was thermostable and non-proteinaceous in nature. S073 was found to produce carboxylate-type siderophores during most of its life cycle using a chrome azurol S assay. The antagonistic activity of S073 was mostly attributed to its higher ability to compete for iron compared with that of V. parahaemolyticus, as deduced from siderophore quantification. Iron supplementation studies indicated that additional mechanisms besides iron competition were simultaneously involved in governing the observed inhibition. Co-culture analysis indicated that S073, although disadvantaged in growth rate, was still competitive in inhibiting vibrios. The promising potential of S073 development as a biocontrol agent in aquaculture was discussed.
KeywordsVibrio parahaemolyticus Marine Streptomyces Antagonistic activity Siderophore Aquaculture Streptomyces Vibrios
This work was supported by the National Natural Science Foundation of China (No. 31300046), the Natural Science Foundation of Guangdong Province (Nos. S2013010013705, 2015A030313319, 2018A030313578), Guangdong Marine and Fishery Bureau Science and Technology Project (No. A201601B05), and the Science and Technology Program of Guangzhou China (No. 201604020029)
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflict of interest.
- Goarant C, Merien F, Berthe F (1999) Arbitrarily primed PCR to type Vibrio spp. pathogenic for shrimp. Appl Environ Microbiol 65:1145–1151Google Scholar
- Huang Y, Zhang L, Tiu L (2015) Characterization of antibiotic resistance in commensal bacteria from an aquaculture ecosystem. Front Microbiol 6:914Google Scholar
- Kieser T, Bibb MJ, Chater K (2000) Practical Streptomyces genetics. The John Innes Foundation, NorwichGoogle Scholar
- Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115–175Google Scholar
- Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
- Selvakumar D, Arun K, Suguna S (2010) Bioactive potential of Streptomyces against fish and shellfish pathogens. Iran J Microbiol 2:157–164Google Scholar
- Tan LT, Chan KG, Lee LH (2016) Streptomyces bacteria as potential probiotics in aquaculture. Front Microbiol 7:79Google Scholar
- Yang N, Sun C (2016) The inhibition and resistance mechanisms of actinonin, isolated from marine Streptomyces sp. NHF165, against Vibrio anguillarum. Front Microbiol 7:1467Google Scholar
- Yeole RD, Dave BP, Dube HC (2001) Siderophore production by fluorescent pseudomonads colonizing roots of certain crop plants. Indian J Exp Biol 39:464–468Google Scholar