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Fisheries Science

, Volume 85, Issue 3, pp 533–543 | Cite as

Antagonistic activity of marine Streptomyces sp. S073 on pathogenic Vibrio parahaemolyticus

  • Mingxia Yang
  • Jun Zhang
  • Qiting Liang
  • Guanxin Pan
  • Jiachang Zhao
  • Miao Cui
  • Xinqing Zhao
  • Qizhong Zhang
  • Delin XuEmail author
Original Article Aquaculture

Abstract

Marine Streptomyces sp. isolate S073 was shown to have strong antagonistic activity towards the pathogenic Vibrio parahaemolyticus using the agar diffusion method. The antagonistic substance(s) secreted into the supernatant was thermostable and non-proteinaceous in nature. S073 was found to produce carboxylate-type siderophores during most of its life cycle using a chrome azurol S assay. The antagonistic activity of S073 was mostly attributed to its higher ability to compete for iron compared with that of V. parahaemolyticus, as deduced from siderophore quantification. Iron supplementation studies indicated that additional mechanisms besides iron competition were simultaneously involved in governing the observed inhibition. Co-culture analysis indicated that S073, although disadvantaged in growth rate, was still competitive in inhibiting vibrios. The promising potential of S073 development as a biocontrol agent in aquaculture was discussed.

Keywords

Vibrio parahaemolyticus Marine Streptomyces Antagonistic activity Siderophore Aquaculture Streptomyces Vibrios 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31300046), the Natural Science Foundation of Guangdong Province (Nos. S2013010013705, 2015A030313319, 2018A030313578), Guangdong Marine and Fishery Bureau Science and Technology Project (No. A201601B05), and the Science and Technology Program of Guangzhou China (No. 201604020029)

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Supplementary material

12562_2019_1309_MOESM1_ESM.docx (101 kb)
Supplementary material 1 (DOCX 100 kb)

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Copyright information

© Japanese Society of Fisheries Science 2019

Authors and Affiliations

  • Mingxia Yang
    • 1
  • Jun Zhang
    • 1
  • Qiting Liang
    • 1
  • Guanxin Pan
    • 1
  • Jiachang Zhao
    • 1
  • Miao Cui
    • 1
  • Xinqing Zhao
    • 2
  • Qizhong Zhang
    • 1
  • Delin Xu
    • 1
    Email author
  1. 1.Department of Ecology, Institute of Hydrobiology, School of Life Science and Technology, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of EducationJinan UniversityGuangzhouPeople’s Republic of China
  2. 2.State Key Laboratory of Microbial Metabolism and School of Life Science and BiotechnologyShanghai Jiao Tong UniversityShanghaiChina

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