Fisheries Science

, Volume 85, Issue 1, pp 187–197 | Cite as

Effect of Bacillus subtilis on intestinal apoptosis of grass carp Ctenopharyngodon idella orally challenged with Aeromonas hydrophila

  • Ding Zhang
  • Zhixin Wu
  • Xiaoxuan ChenEmail author
  • Huan Wang
  • Daoyuan Guo
Original Article Aquaculture


The influence of Bacillus subtilis Ch9 on intestinal apoptosis of grass carp Ctenopharyngodon idella challenged with Aeromonas hydrophila was studied. Groups of grass carp were orally intubated with B. subtilis or phosphate buffered saline for 3 days and subjected to oral A. hydrophila challenge. Intestinal tissues were collected and prepared for terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay, real-time quantitative polymerase chain reaction analysis of caspase-3 and -8 and b-cell lymphoma–2 protein (bcl-2), histopathological analyses and measurement of caspase-3 and -8 activities at 0, 12, 24, 48, 72 and 96 h post-challenge. TUNEL analyses demonstrated that B. subtilis induced intestinal cell apoptosis, and caspase-3 and -8 activities increased significantly compared to in the control and A. hydrophila groups. The intestinal damage due to A. hydrophila was also reduced. However, messenger RNA transcript levels of caspase-3 and -8 and bcl-2 were not markedly elevated. These results suggested that B. subtilis Ch9 increased intestinal apoptosis in fish challenged with A. hydrophila, and that B. subtilis Ch9-induced caspase-dependent apoptosis may play an important role in reducing intestinal damage following A. hydrophila challenge.


Intestine Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling Real-time quantitative polymerase chain reaction Caspase Aquaculture 



This work was supported by the National Natural Science Foundation of China (grant nos. 31472310, 31672683).


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Copyright information

© Japanese Society of Fisheries Science 2018

Authors and Affiliations

  1. 1.Hubei Engineering Technology Research Center for Aquatic Animal Diseases Control and Prevention, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, College of FisheriesHuazhong Agricultural UniversityWuhanPeople’s Republic of China

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