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Food and Environmental Virology

, Volume 11, Issue 1, pp 52–64 | Cite as

Detection of Human Enteric Viruses in French Polynesian Wastewaters, Environmental Waters and Giant Clams

  • Laetitia Kaas
  • Leslie Ogorzaly
  • Gaël Lecellier
  • Véronique Berteaux-Lecellier
  • Henry-Michel Cauchie
  • Jérémie LangletEmail author
Original Paper
  • 35 Downloads

Abstract

Lack of wastewater treatment efficiency causes receiving seawaters and bivalve molluscan shellfish to become contaminated, which can lead to public health issues. Six wastewater samples, five seawater samples and three batches of giant clams from Tahiti (French Polynesia) were investigated for the presence of enteric viruses, but also if present, for the diversity, infectivity and integrity of human adenoviruses (HAdV). Enteroviruses (EV), sapoviruses (SaV) and human polyomaviruses (HPyV) were detected in all wastewater samples. In decreasing frequency, noroviruses (NoV) GII and HAdV, rotaviruses (RoV), astroviruses (AsV), NoV GI and finally hepatitis E viruses (HEV) were also observed. Nine types of infectious HAdV were identified. HPyV and EV were found in 80% of seawater samples, NoV GII in 60%, HAdV and SaV in 40% and AsV and RoV in 20%. NoV GI and HEV were not detected in seawater. Intact and infectious HAdV-41 were detected in one of the two seawater samples that gave a positive qPCR result. Hepatitis A viruses were never detected in any water types. Analysis of transcriptomic data from giant clams revealed homologues of fucosyltransferases (FUT genes) involved in ligand biosynthesis that strongly bind to certain NoV strains, supporting the giant clams ability to bioaccumulate NoV. This was confirmed by the presence of NoV GII in one of the three batches of giant clams placed in a contaminated marine area. Overall, all sample types were positive for at least one type of virus, some of which were infectious and therefore likely to cause public health concerns.

Keywords

Noroviruses Enteric viruses Adenoviruses Wastewater Recreational water Giant clams Infectivity Next-generation sequencing 

Notes

Acknowledgements

This work has been undertaken as part of a research project co-funded by the Pacific Fund of the French Ministry of Foreign and European Affairs, project No. 07 / 2015. The authors want to thank the Embassy of France in New Zealand, Stéphane Lastère (Centre Hospitalier de Polynésie française—CHPf) as well Maréva Vigneron and Glenda Melix (Centre d’Hygiène et de Salubrité Publique—CHSP) for advice on study site selection and water samplings. Special thanks to Joanne Hewitt (Institute of Environmental Science and Research—ESR) for her valuable assistance and Cécile Walczak and Delphine Collard (Luxembourg Institute of Science and Technology—LIST) for their precious and excellent technical assistance. Joanne Hewitt and Pradip Gyawali (Institute of Environmental Science and Research—ESR) are also acknowledged for their critical review of the manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical Approval

The manuscript does not contain clinical studies or patient data.

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Authors and Affiliations

  1. 1.Enteric, Environmental and Food Virology LaboratoryInstitute of Environmental Science and Research (ESR)PoriruaNew Zealand
  2. 2.Department of Environmental Research and Innovation (ERIN)Luxembourg Institute of Science and Technology (LIST)BelvauxLuxembourg
  3. 3.PSL CRIOBE USR3278 CNRS-EPHE-UPVD, Labex CORAILMooreaFrench Polynesia
  4. 4.Département de BiologieUniversité de Paris Saclay UVSQVersaillesFrance
  5. 5.UMR250/9220 ENTROPIE IRD-CNRS-UR, Labex CORAILNoumea CedexFrance

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