Biophysical Reviews

, Volume 11, Issue 3, pp 395–398 | Cite as

Comparison of two different partially fluorinated phosphatidylcholines with the perfluorobutyl group on thermotropic properties of the bilayer membrane and reconstituted bacteriorhodopsin

  • Tamami Yanagi
  • Toshiyuki TakagiEmail author
  • Hiroshi TakahashiEmail author
  • Takashi Kikukawa
  • Hideki Amii
  • Masashi SonoyamaEmail author
Letter to the Editor


Fluorinated surfactants (Shepherd and Holzenburg 1995; Popot 2010; Frotscher et al. 2015) and phospholipids (Santaella et al. 1991; Yoder et al. 2007; Takai et al. 2008; Gagnon et al. 2018) are now attracting more and more attention in biophysical fields because the incorporation of fluorine atoms provides distinctive physical properties to amphiphilic molecules, which would open up a new way of structural and functional characterization of membrane proteins (MPs). In particular, a novel partially fluorinated analog of dimyristoylphosphatidylcholine (DMPC), F4-DMPC (Fig.  1, m = 9; diF4H10-PC was used as the former abbreviation in the previous papers) (Yoshino et al. 2012; Takahashi et al. 2013; Okamoto et al. 2016), is of great promise for biophysical studies of MPs. Bacteriorhodopsin (bR) molecules in the F4-DMPC bilayer membrane form the native purple membrane (PM)–like two-dimensional crystals of trimers as the structural unit in the liquid-crystalline phase as well as...



We would like to thank Ms. Maria Takahashi for sample preparation and CD measurements of bR/F4-DPPC.

Funding information

This work was partly supported by Grants-in-Aid for Scientific Research (KAKENHI: 15K05558 to M.S., 18K06568 to T.T., 16K05509 to H.T., and 18H04381 to H.A.) from the Japan Society for the Promotion of Science (JSPS).

Compliance with ethical standards

Conflict of interest

Tamami Yanagi declares that she has no conflict of interest. Toshiyuki Takagi declares that he has no conflict of interest. Hiroshi Takahashi declares that he has no conflict of interest. Takashi Kikukawa declares that he has no conflict of interest. Hideki Amii declares that he has no conflict of interest. Masashi Sonoyama declares that he has no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of Molecular Science, Faculty of Science and TechnologyGunma UniversityKiryuJapan
  2. 2.Biotechnology Research Institute for Drug DiscoveryNational Institute of Advanced Industrial Science and Technology (AIST)TsukubaJapan
  3. 3.Division of Pure and Applied Science, Graduate School of Science and TechnologyGunma UniversityMaebashiJapan
  4. 4.Faculty of Advanced Life ScienceHokkaido UniversitySapporoJapan
  5. 5.Global Station for Soft Matter, GI-CoREHokkaido UniversitySapporoJapan
  6. 6.Gunma University Center for Food Science and Wellness (GUCFW)Gunma UniversityMaebashiJapan
  7. 7.Gunma University Initiative for Advanced Research (GIAR)Gunma UniversityMaebashiJapan

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