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Biophysical Reviews

, Volume 11, Issue 3, pp 311–318 | Cite as

Tensile and compressive force regulation on cell mechanosensing

  • Yunfeng Chen
  • Zhiyong Li
  • Lining Arnold JuEmail author
Review

Abstract

Receptor-mediated cell mechanosensing plays critical roles in cell spreading, migration, growth, and survival. Dynamic force spectroscopy (DFS) techniques have recently been advanced to visualize such processes, which allow the concurrent examination of molecular binding dynamics and cellular response to mechanical stimuli on single living cells. Notably, the live-cell DFS is able to manipulate the force “waveforms” such as tensile versus compressive, ramped versus clamped, static versus dynamic, and short versus long lasting forces, thereby deriving correlations of cellular responses with ligand binding kinetics and mechanical stimulation profiles. Here, by differentiating extracellular mechanical stimulations into two major categories, tensile force and compressive force, we review the latest findings on receptor-mediated mechanosensing mechanisms that are discovered by the state-of-the-art live-cell DFS technologies.

Keywords

Mechanosensing Receptor–ligand interactions Dynamic force spectroscopy Force waveform 

Abbreviations

DFS

Dynamic force spectroscopy

AFM

Atomic force microscopy

BFP

Biomembrane force probe

OT

Optical tweezers

TCR

T cell receptor

pMHC

Peptide major histocompatibility complex

VWF

von Willebrand factor

Notes

Acknowledgments

We thank Prof. Cheng Zhu for helpful discussion. This work was supported by the Cardiac Society of Australia and New Zealand BAYER Young Investigator Research Grant (L.A.J.). L.A.J. is an Australian Research Council DECRA Fellow (DE190100609) and a former National Heart Foundation of Australia postdoctoral fellow (101798). Y.C. is a MERU (Medolago-Ruggeri) Foundation post-doctoral awardee. Z.L. is an Australian Research Council Future Fellow (FT140101152).

Compliance with ethical standards

Conflict of interest

Yunfeng Chen declares that he has no conflict of interest. Zhiyong Li declares that he has no conflict of interest. Lining Arnold Ju declares that he has no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Molecular Medicine, MERU-Roon Research Center on Vascular BiologyThe Scripps Research InstituteLa JollaUSA
  2. 2.School of Chemistry, Physics and Mechanical EngineeringQueensland University of TechnologyBrisbaneAustralia
  3. 3.Heart Research InstituteSydneyAustralia
  4. 4.School of Aerospace, Mechanical and Mechatronic EngineeringDarlingtonAustralia
  5. 5.Charles Perkins CentreThe University of SydneyCamperdownAustralia

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