Tensile and compressive force regulation on cell mechanosensing
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Receptor-mediated cell mechanosensing plays critical roles in cell spreading, migration, growth, and survival. Dynamic force spectroscopy (DFS) techniques have recently been advanced to visualize such processes, which allow the concurrent examination of molecular binding dynamics and cellular response to mechanical stimuli on single living cells. Notably, the live-cell DFS is able to manipulate the force “waveforms” such as tensile versus compressive, ramped versus clamped, static versus dynamic, and short versus long lasting forces, thereby deriving correlations of cellular responses with ligand binding kinetics and mechanical stimulation profiles. Here, by differentiating extracellular mechanical stimulations into two major categories, tensile force and compressive force, we review the latest findings on receptor-mediated mechanosensing mechanisms that are discovered by the state-of-the-art live-cell DFS technologies.
KeywordsMechanosensing Receptor–ligand interactions Dynamic force spectroscopy Force waveform
Dynamic force spectroscopy
Atomic force microscopy
Biomembrane force probe
T cell receptor
Peptide major histocompatibility complex
von Willebrand factor
We thank Prof. Cheng Zhu for helpful discussion. This work was supported by the Cardiac Society of Australia and New Zealand BAYER Young Investigator Research Grant (L.A.J.). L.A.J. is an Australian Research Council DECRA Fellow (DE190100609) and a former National Heart Foundation of Australia postdoctoral fellow (101798). Y.C. is a MERU (Medolago-Ruggeri) Foundation post-doctoral awardee. Z.L. is an Australian Research Council Future Fellow (FT140101152).
Compliance with ethical standards
Conflict of interest
Yunfeng Chen declares that he has no conflict of interest. Zhiyong Li declares that he has no conflict of interest. Lining Arnold Ju declares that he has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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