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Biophysical Reviews

, Volume 11, Issue 3, pp 327–333 | Cite as

“Force-From-Lipids” mechanosensation in Corynebacterium glutamicum

  • Yoshitaka Nakayama
  • Ken-ichi Hashimoto
  • Hisashi Kawasaki
  • Boris MartinacEmail author
Review

Abstract

Since the mechanosensitive channel MscCG has been identified as the major glutamate efflux system in Corynebacterium glutamicum, studies of mechanotransduction processes in this bacterium have helped to unpuzzle a long-unresolved mystery of glutamate efflux that has been utilised for industrial monosodium glutamate production. The patch clamp recording from C. glutamicum giant spheroplasts revealed the existence of three types of mechanosensitive (MS) channels in the cell membrane of this bacterium. The experiments demonstrated that the MS channels could be activated by membrane tension, indicating that the channel gating by mechanical force followed the “Force-From-Lipids (FFL)” principle characteristic of ion channels inherently sensitive to transbilayer pressure profile changes in the mechanically stressed membrane bilayer. Mechanical properties of the C. glutamicum membrane are characteristics of very soft membranes, which in the C. glutamicum membrane are due to negatively charged lipids as its exclusive constituents. Given that membrane lipids are significantly altered during the fermentation process in the monosodium glutamate production, MS channels seem to respond to changes in force transmission through the membrane bilayer due to membrane lipid dynamics. In this review, we describe the recent results describing corynebacterial FFL-dependent mechanosensation originating from the particular lipid composition of the C. glutamicum membrane and unique structure of MscCG-type channels.

Keywords

NCgl1221 MscS Mechanosensation Glutamate efflux Fatty acid synthesis 

Notes

Acknowledgements

We acknowledge the Japanese Society for Promotion of Science (JSPS) for a fellowship to YN and the National Health and Medical Research Council of Australia for a Principal Research Fellowship to BM.

Author contributions

YN and BM wrote the manuscript. YN, HK, KH, and BM contributed to editing.

Compliance with ethical standards

Conflict of interest

Yoshitaka Nakayama declares that he has no conflict of interest. Ken-ichi Hashimoto declares that he has no conflict of interest. Hisashi Kawasaki declares that he has no conflict of interest. Boris Martinac declares that he has no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Molecular Cardiology and Biophysics DivisionVictor Chang Cardiac Research InstituteDarlinghurstAustralia
  2. 2.Biotechnology Research CenterThe University of TokyoTokyoJapan
  3. 3.Collaborative Research Institute for Innovative MicrobiologyThe University of TokyoTokyoJapan
  4. 4.St Vincent’s Clinical School, Faculty of MedicineUniversity of New South WalesDarlinghurstAustralia

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