Cytoskeletal discoveries in the plant lineage using the moss Physcomitrella patens
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Advances in cell biology have been largely driven by pioneering work in model systems, the majority of which are from one major eukaryotic lineage, the opisthokonts. However, with the explosion of genomic information in many lineages, it has become clear that eukaryotes have incredible diversity in many cellular systems, including the cytoskeleton. By identifying model systems in diverse lineages, it may be possible to begin to understand the evolutionary origins of the eukaryotic cytoskeleton. Within the plant lineage, cell biological studies in the model moss, Physcomitrella patens, have over the past decade provided key insights into how the cytoskeleton drives cell and tissue morphology. Here, we review P. patens attributes that make it such a rich resource for cytoskeletal cell biological inquiry and highlight recent key findings with regard to intracellular transport, microtubule-actin interactions, and gene discovery that promises for many years to provide new cytoskeletal players.
KeywordsActin Microtubules Myosin Kinesin Organelle transport Tip growth Phragmoplast
We thank Xiaohang Cheng for careful reading of the manuscript.
MY was supported by a Japan Society for the Promotion of Science pre-doctoral fellowship (16J02796). A grant from the National Science Foundation (MCB-1715785) supported S-Z W and MB. DM and MB were supported by funds from Dartmouth College.
Compliance with ethical standards
Conflict of interest
Shu-Zon Wu declares that she has no conflict of interest. Moe Yamada declares that she has no conflict of interest. Darren R. Mallett declares that he has no conflict of interest. Magdalena Bezanilla declares that she has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Doonan JH, Cove DJ, Lloyd CW (1988) Microtubules and microfilaments in tip growth: evidence that microtubules impose polarity on protonemal growth in Physcomitrella patens. J Cell Sci 89:533–540Google Scholar
- Doonan JH, Cove DJ, Lloyd CW (1985) Immunofluorescence microscopy of microtubules in intact cell lineages of the moss I Normal and CIPC-treated tip cells. J Cell Sci 75:131–147Google Scholar
- Heslop-Harrison J, Heslop-Harrison Y, Cresti M et al (1988) Cytoskeletal elements, cell shaping and movement in the angiosperm pollen tube. J Cell Sci 91:49–60Google Scholar
- Jonsson E, Yamada M, Vale RD, Goshima G (2015) Clustering of a kinesin-14 motor enables processive retrograde microtubule-based transport in plants. Nature Plants https://doi.org/10.1038/NPLANTS.2015.87
- Kumar AS, Park E, Nedo A, et al (2018) Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity. elife https://doi.org/10.7554/eLife.23625
- Rounds CM, Bezanilla M (2013) Growth mechanisms in tip-growing plant cells. Annu Rev Plant Biol 64:243–265. https://doi.org/10.1146/annurev-arplant-050312-120150 CrossRefGoogle Scholar
- Sato Y, Wada M, Kadota A (2001) Choice of tracks, microtubules and/or actin filaments for chloroplast photo-movement is differentially controlled by phytochrome and a blue light receptor. J Cell Sci 114:269–279Google Scholar
- Schaefer DG, Zrÿd J-P (1997) Efficient gene targeting in the moss Physcomitrella patens. Plant J 11:1195–1206. https://doi.org/10.1046/j.1365-313X.1997.11061195.x CrossRefGoogle Scholar
- Stevenson SR, Kamisugi Y, Trinh CH et al (2016) Genetic analysis of Physcomitrella patens identifies ABSCISIC ACID NON-RESPONSIVE, a regulator of ABA responses unique to basal land plants and required for desiccation tolerance. Plant Cell 28:1310–1327. https://doi.org/10.1105/tpc.16.00091 CrossRefPubMedPubMedCentralGoogle Scholar
- Strepp R, Scholz S, Kruse S et al (1998) Plant nuclear gene knockout reveals a role in plastid division for the homolog of the bacterial cell division protein FtsZ, an ancestral tubulin. Proc Natl Acad Sci U S A 95:4368–4373. https://doi.org/10.1073/pnas.95.8.4368 CrossRefPubMedPubMedCentralGoogle Scholar
- Yamada M, Tanaka-Takiguchi Y, Hayashi M, et al (2017) Multiple kinesin-14 family members drive microtubule minus end-directed transport in plant cells. J Cell Biol jcb.201610065. https://doi.org/10.1083/jcb.201610065
- Zurzycki J (1980) Blue light-induced intracellular movements. 50–68. https://doi.org/10.1007/978-3-642-67648-2_6