Biophysical Reviews

, Volume 10, Issue 4, pp 949–954 | Cite as

Non-sarcomeric causes of heart failure: a Sydney Heart Bank perspective

  • C. G. dos RemediosEmail author
  • A. Li
  • S. Lal

The Sydney Heart Bank (SHB) has contributed and continues to contribute samples of human left ventricles to our understanding of molecular mechanisms underlying heart failure (Li et al. 2013; Li and dos Remedios 2015). The great majority of the publications concern myofibrillar proteins. To date, the SHB has published about 120 research papers with about 60 collaborators around the world. About 20% of them involved genes and proteins that did not involve sarcomeric proteins.

Working with the SHB

The authors took the position that we should not expect to identify all of the causes of heart failure mechanisms, and that the best way forward would be to collaborate with the many laboratories in searching for an answer. The major obstacle to this aspiration was the need to convince these labs that your heart samples were unique in several ways. First we had to dispel the notion that our samples were degraded, or at least partially degraded due to the time between harvesting and preservation...


Compliance with ethical standards

Conflict of interest

C. G. dos Remedios declares that he has no conflicts of interest. A. Li declares that she has no conflicts of interest. S. Lal declares that he has no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


  1. Berry D, Yao M, Barden JA, Balcar VJ, Hansen MA (1998) Alterations in the expression of P2X1 receptors in failing and non-diseased human atria. Electrophoresis 19:856–859CrossRefPubMedGoogle Scholar
  2. Berry D, Barden JA, Balcar VJ, Keogh A, dos Remedios CG (1999) Increase in expression of P2X1 receptors in the atria of patients suffering from dilated cardiomyopathy. Electrophoresis 20:2059–2064CrossRefPubMedGoogle Scholar
  3. Berry D, Balcar VJ, Barden JA, Keogh A, dos Remedios CG (2000) Determination of P2X1α-sarcoglycan (adhalin) expression levels in failing human dilated cardiomyopathic left ventricles changes in P2X1 receptors in human left ventricles and their relationship to the acto-ATPase α-sarcoglycan (adhalin). Electrophoresis 21:3857–3862CrossRefPubMedGoogle Scholar
  4. Berry DA, Keogh A, dos Remedios CG (2001) Nuclear membrane proteins in failing human dilated cardiomyopathy. Proteomics 1:1507–1512CrossRefPubMedGoogle Scholar
  5. Boateng SY, Belin RJ, Geenen DL, Margulies KB, Martin JL et al (2007) Cardiac dysfunction and heart failure are associated with abnormalities in the subcellular distribution and amounts of oligomeric muscle LIM protein. Am J Physiol Heart Circ Physiol 292:H259–H269CrossRefPubMedGoogle Scholar
  6. Bos JM, Poley RN, Ny M, Tester DJ, Xu X et al (2006) Genotype-phenotype relationships involving hypertrophic cardiomyopathy-associated mutations in titin, muscle LIM protein, and telethonin. Mol Genet Metab 88:78–85CrossRefPubMedGoogle Scholar
  7. Bovill E, Westaby S, Crisp A, Jacobs S, Shaw T (2008) Reduction of four-and-a-half LIM-protein 2 expression occurs in human left ventricular failure and leads to altered localization and reduced activity of metabolic enzymes. J Thorac Cardiovasc Surg 137:853–861. CrossRefGoogle Scholar
  8. Chahine MN, Mioulane M, Sikkel MB, O’Gara P, dos Remedios CG et al (2014) Nuclear pore rearrangements and nuclear trafficking in cardiomyocytes from rat and human failing hearts. Cardiovasc Res 105:31–43. CrossRefPubMedPubMedCentralGoogle Scholar
  9. Court NW, dos Remedios CG, Cordell J, Bogoyevitch MA (2002) Cardiac expression and subcellular localization of the p38 mitogen-activated protein kinase member, stress-activated protein kinase-3 (SAPK3). J Mol Cell Cardiol 34:413–426CrossRefPubMedGoogle Scholar
  10. Crossman DJ, Shen X, Jüllig M, Munro M, Hou Y et al (2017) Increased collagen within the transverse tubules in human heart failure. Cardiovasc Res 113:879–891. CrossRefPubMedGoogle Scholar
  11. Dwyer J, Pluess M, Iskratsch T, dos Remedios CG, Ehler E (2015) The formin FHOD1 in cardiomyocytes. Anat Rec 297:1560–1570CrossRefGoogle Scholar
  12. Fatkin D, MacRae C, Sasaki T, Wolff MR, Porcu M et al (1999) Misspence mutations in the rod domain of the lamin A/C gene as causes of dilated cardiomyopathy and conduction system disease. N Engl J Med 341:1715–1724CrossRefPubMedGoogle Scholar
  13. Hou M, Malmsjö M, Möller S, Pantev E, Bergdahl A, Zhao XH, Sun XY, Hedner T, Edvinsson L, Erling D (1999) Increase in cardiac P2X1- and P2Xy2-receptor mRNA levels in congestive heart failure. Life Sci 65:1195–1206CrossRefPubMedGoogle Scholar
  14. Huang Z-P, Ding Y, Chen J, Wu G, Kataoka M, Wang D-Z et al (2016) Long non-coding RNAs link extracellular matrix gene expression to ischemic cardiomyopathy. Cardiovasc Res 112:543–554. CrossRefPubMedCentralPubMedGoogle Scholar
  15. Iskratsch T, Lange S, Kho AL, dos Remedios CG, Ehler E (2010) Formin follows function: a muscle specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. J Cell Biol 191:1159–1172CrossRefPubMedPubMedCentralGoogle Scholar
  16. Lal S, Li A, dos Remedios C (2016) Limitations in translating animal studies to humans in cardiovascular disease. J Cardiovasc Transl Res 9:165–166. CrossRefPubMedGoogle Scholar
  17. Lange S, Gehmlich K, Lun AS, Blondelle J, Hooper C et al (2016) MLP and CARP are linked to chronic PKCa signalling in dilated cardiomyopathy. Nat Commun 7:12120. CrossRefPubMedPubMedCentralGoogle Scholar
  18. Li A, dos Remedios CG (2015) Special issue on human heart failure. Biophys Rev 7:1–3CrossRefPubMedPubMedCentralGoogle Scholar
  19. Li A, Ponten F, dos Remedios CG (2012) The interactome of Lim domain proteins: the contributions of LIM domain proteins to heart failure and heart development. Proteomics 12:203–225. CrossRefPubMedGoogle Scholar
  20. Li A, Estigoy C, Raftery M, Cameron D, Odeberg J et al (2013) Heart research advances using database search engines, human protein atlas and the Sydney Heart Bank. Heart Lung Circ 22:819–826CrossRefPubMedGoogle Scholar
  21. Lin Z, Guo H, Cao Y, Zohrabian S, Zhou P et al (2016) Acetylation of VGLL4 regulates hippo-YAP regulates growth-YAP signaling and postnatal growth. Dev Cell 39:466–479. CrossRefPubMedPubMedCentralGoogle Scholar
  22. Lu B, Yu H, Zwartbol M, Ruifrok WP, van Gilst WH et al (2012) Identification of hypertrophy- and heart failure-associated genes by combining in vitro and in vivo models. Physiol Genomics 44:443–454. CrossRefPubMedGoogle Scholar
  23. Ng DCH, Court NW, dos Remedios CG, Bogoyevitch MA (2003) Activation of signal transducer and activator of transcription (STAT) pathways in failing human hearts. Cardiovasc Res 57:333–346CrossRefPubMedGoogle Scholar
  24. Pleuss M, Daeubler G, dos Remedios CG, Ehler E (2015) Adaptations of cytoarchitecture in human dilated cardiomyopathy. Biophys Rev 7:25–32CrossRefGoogle Scholar
  25. Polden J, McManus CA, dos Remedios C, Dunn MJ (2011) A 2-D gel reference map of the basic human heart proteome. Proteomics 11:3582–3586CrossRefPubMedGoogle Scholar
  26. Ralevic C (2015) P2X receptors in the cardiovascular system and their potential as therapeutic targets in disease. Curr Med Chem 22:851–865CrossRefPubMedGoogle Scholar
  27. Robinson AA, Dunn MJ, McCormack A, dos Remedios CG, Rose ML (2010) Protective effect of phosphorylated Hsp27 in coronary arteries through actin stabilization. J Mol Cell Cardiol 49:370–379CrossRefPubMedGoogle Scholar
  28. Schreckenbach T, Henn W, Kress W, Roos A, Maschke M et al (2013) Novel FHL1 mutation in a family with reducing body myopathy. Muscle Nerve 47:127–134. CrossRefPubMedGoogle Scholar
  29. Walweel K, Li J, Molenaar P, Imtiaz MS, Quail A et al (2014) Regulation of human RyR2 by Ca2+ and Mg2+ in the cytoplasm and in the lumen of the sarcoplasmic reticulum. J Gen Physiol 144:263–271CrossRefPubMedPubMedCentralGoogle Scholar
  30. Walweel K, Gomez-Hurtado N, Oo YW, Beard NA, dos Remedios C et al (2017a) Calmodulin mutants linked to catecholaminergic polymorphic ventricular tachycardia fail to inhibit human RyR2 channels during adrenergic stress. J Am Coll Cardiol 70:115–117. CrossRefPubMedGoogle Scholar
  31. Walweel K, Molenaar P, Imtiaz MS, Denniss A, dos Remedios C et al (2017b) Ryanodine receptor modification and regulation by intracellular Ca2+ and Mg2+ in healthy and failing human hearts. J Mol Cell Cardiol 104:263–271. CrossRefGoogle Scholar

Copyright information

© International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Sydney Heart Bank, Bosch Institute, Anderson Stuart Building (F13)University of SydneySydneyAustralia

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