Biophysical Reviews

, Volume 10, Issue 2, pp 285–292 | Cite as

High-speed atomic force microscopy and its future prospects

Review

Abstract

Various techniques have been developed and used to investigate how proteins produce complex biological architectures and phenomena. Among these techniques, high-speed atomic force microscopy (HS-AFM) holds a unique position. It is only HS-AFM that allows the simultaneous assessment of structure and dynamics of single protein molecules in action. This new microscopy tool has been successfully applied to a variety of proteins, from motor proteins to membrane proteins, antibodies, enzymes, and even to intrinsically disordered proteins. And yet there still remain many biomolecular phenomena that cannot be addressed by HS-AFM in its current form. Here, I present a brief history of HS-AFM development, describe the current state of HS-AFM, and then discuss which new biological scanning probe microscopy techniques will be coming up next.

Keywords

Imaging High-speed AFM Proteins Dynamic processes Structural changes 

Notes

Acknowledgements

This work was supported by JST/CREST (#JPMJCR13M1) and KAKENHI from the Ministry of Education, Culture, Sports, Science and Technology, Japan (#21113002, #24227005 and #26119003).

Compliance with ethical standards

Conflicts of interest

Toshio Ando declares that he has no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by the author.

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Copyright information

© International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Nano-Life Science InstituteKanazawa UniversityKanazawaJapan
  2. 2.Core Research for Evolutionary Science and Technology (CREST) Japan Science and Technology Agency (JST)TokyoJapan

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