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Pseudomonas simiae effects on the mycotoxin formation by fusaria and alternaria in vitro and in a wheat field

  • Thomas MüllerEmail author
  • Peter Lentzsch
  • Undine Behrendt
  • Dietmar Barkusky
  • Marina E. H. Müller
Original Article

Abstract

Fluorescent pseudomonads colonizing wheat ears have a high antagonistic potential against phytopathogenic fungi. To check this hypothesis, the bacterial antagonist Pseudomonas simiae 9 rif+/kan+ was spray-inoculated onto the ears of winter wheat in a locally demarcated experimental field plot. Fusarium and Alternaria fungi naturally occurring on the ears and the formation of their mycotoxins in the ripe grains were investigated. Inoculated bacteria were recovered from the plants in the inoculation cell, but not in the untreated neighboring plots or in the air above the plants. Growth of fusaria and alternaria on the ears was not influenced by the bacterial antagonist. Wheat kernels were co-inoculated in vitro with the antagonist and one mycotoxin-producing strain of Fusarium and Alternaria, respectively. Mycotoxin production was almost completely suppressed in these approaches. Concentrations of zearalenone, deoxynivalenol, alternariol, and tenuazonic acid were also significantly reduced in ripe grains in the field, but to a lesser extent than in vitro. The results of this and previous studies suggest that widespread biological control of the growth of fusaria and alternaria and their mycotoxin formation by naturally occurring pseudomonads with antagonistic activity is rather unlikely.

Keywords

Biological control Wheat Pseudomonas Fusarium Alternaria Mycotoxins 

Abbreviations

ALT

Altenuene

AME

Alternariol monomethyl ether

AOH

Alternariol

CFU

Colony-forming units

DON

Deoxynivalenol

DM

Dry mass

FM

Fresh mass

HPLC

High-performance liquid chromatography

MBCA

Microbial biocontrol agents

NIV

Nivalenol

TeA

Tenuazonic acid

ZEN

Zearalenone

Notes

Acknowledgments

We thank Petra Lange, Martina Peters, and Grit von der Waydbrink, for excellent technical assistance.We are also grateful to E. Garcia-Valdes and J. Lalucat (Balearic Islands University) for rpoD gene sequencing of the antagonist used in this study.

Source of funding

This work was financially supported by Deutsche Forschungsgemeinschaft in the framework of the BioMove Research Training Group (DFG-GRK 2118/1).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

12550_2019_379_MOESM1_ESM.pptx (74 kb)
ESM_1. pptx: Field plot design. (PPTX 74 kb)
12550_2019_379_MOESM2_ESM.pptx (89 kb)
ESM_2. pptx: Temperature and rainfall during field investigations in 2017 and 2018 (PPTX 88 kb)
12550_2019_379_MOESM3_ESM.xlsx (20 kb)
ESM_3. xlsx:In vitro-antagonism test on a natural substrate with Fusarium graminearum 23 and Pseudomonas simiae 9rif+/kan+. Microbiological and mycotoxin data (XLSX 20 kb)
12550_2019_379_MOESM4_ESM.xlsx (19 kb)
ESM_4. xlsx:In vitro-antagonism test on a natural substrate with Alternaria tenuissima 220 and Pseudomonas simiae 9rif+/kan+. Microbiological and mycotoxin data (XLSX 19 kb)
12550_2019_379_MOESM5_ESM.xlsx (31 kb)
ESM_5. xlsx: Field experiments in 2017 and 2018. Microbiological data (XLSX 31 kb)
12550_2019_379_MOESM6_ESM.pptx (238 kb)
ESM_6. pptx: Field experiments. Densities of culturable filamentous fungi on wheat ears. (PPTX 238 kb)

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Copyright information

© Society for Mycotoxin (Research Gesellschaft für Mykotoxinforschung e.V.) and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Leibniz-Centre for Agricultural Landscape Research (ZALF)MünchebergGermany

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