Conceptual Study for Tissue-Regenerative Biodegradable Magnesium Implant Integrated with Nitric Oxide-Releasing Nanofibers
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The excessive initial corrosion rate of Mg is a critical limitation in the clinical application of biodegradable Mg implants because the device loses its fixation strength before the fractured bone heals. This study suggests a new approach to overcome this hurdle by accelerating tissue regeneration instead of delaying the implant biodegradation. As angiogenesis is an essential process in early bone regeneration, a Mg implant coated with electrospun nanofibers containing nitric oxide (NO), which physiologically promotes angiogenesis, is designed. The integrated device enables adjustable amounts of NO to be stored on the NO donor-conjugated nanofiber coating, stably delivered, and released to the fractured bone tissue near the implanted sites. An in vitro corrosion test reveals no adverse effect of the released NO on the corrosion behavior of the Mg implant. Simultaneously, the optimal concentration level of NO released from the implant significantly enhances tube network formation of human umbilical vein endothelial cells without any cytotoxicity problem. This indicates that angiogenesis can be accelerated by combining NO-releasing nanofibers with a Mg implant. With its proven feasibility, the proposed approach could be a novel solution for the initial stability problem of biodegradable Mg implants, leading to successful bone fixation.
KeywordsNitric oxide Nanofiber Angiogenesis Biodegradable magnesium implant Bone regeneration
This research was supported by a grant of the Ministry of Commerce, Industry, and Energy of the Korean Government (Project Number: 10065241) and a grant from the Korea Institute of Science and Technology (2V05460, KIST-Korea University TRC program). This research was also supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Minister of Science, ICT & Future Planning (NRF-2015M3A9E2029186).
- 8.J.-W. Lee, H.-S. Han, K.-J. Han, J. Park, H. Jeon, M.-R. Ok, H.-K. Seok, J.-P. Ahn, K.E. Lee, D.-H. Lee, S.J. Yang, S.Y. Cho, P.R. Cha, H. Kwon, T.H. Nam, J.H. Han, H.J. Rho, K.S. Lee, Y.C. Kim, D. Mantovani, Long-term clinical study and multiscale analysis of in vivo biodegradation mechanism of Mg alloy. Proc. Natl. Acad. Sci. USA 113, 716–721 (2016)CrossRefGoogle Scholar
- 16.P.R. Cha, H.S. Han, G.F. Yang, Y.C. Kim, K.H. Hong, S.C. Lee, J.Y. Jung, J.P. Ahn, Y.Y. Kim, S.Y. Cho, J.Y. Byun, K.S. Lee, S.J. Yang, H.K. Seok, Biodegradability engineering of biodegradable Mg alloys: tailoring the electrochemical properties and microstructure of constituent phases. Sci. Rep. 3, 2367 (2013). https://doi.org/10.1038/srep02367 CrossRefGoogle Scholar
- 38.J. Park, P. Du, J.K. Jeon, G.H. Jang, M.P. Hwang, H.S. Han, K. Park, K.H. Lee, J.W. Lee, H. Jeon, Y.C. Kim, J.W. Park, H.K. Seok, M.-R. Ok, Magnesium corrosion triggered spontaneous generation of H2O2 on oxidized titanium for promoting angiogenesis. Angew. Chem. Int. Ed. Engl. 54, 14753–14757 (2015)CrossRefGoogle Scholar