Advertisement

Marine Biodiversity

, Volume 49, Issue 1, pp 277–287 | Cite as

New data and 3D reconstruction of four species of Pruvotinidae (Mollusca: Solenogastres) from the NW Iberian peninsula

  • Lucía PedrouzoEmail author
  • Óscar García-Álvarez
  • Victoriano Urgorri
  • Marcos Pérez-Señarís
Original Paper
  • 74 Downloads

Abstract

The family Pruvotinidae (Mollusca, Solenogastres, Cavibelonia) includes 34 species, six of which are present in hard bottoms off Galicia (NW Iberian Peninsula). In this study, three-dimensional (3D) anatomical reconstruction techniques were applied for the study of four species of the family present in Galician waters: Pruvotina artabra, Gephyroherpia impar, Luitfriedia minuta and Unciherpia hirsuta. Histology and 3D rendering in this study were carried out with Avizo 6.3 software and micro-computed tomography (micro-CT). New morphological and anatomical data are provided. The 3D reconstruction techniques used, which are based on histological sections, offer a slightly distorted view of the anatomy, but allow a better understanding of the relative situation of the organs. Micro-CT provides less information than histological reconstruction, but offers an excellent 3D anatomical view and allows the specimens to be preserved intact. With the new specimens collected, new data on their distribution and bathymetry are provided.

Keywords

Solenogastres Pruvotinidae Galicia 3D reconstruction Micro-CT 

Notes

Acknowledgements

This work was supported by a scholarship through the predoctoral support program as part of the Galician Plan for Research, Innovation and Growth (Plan I2C) promoted by the Xunta de Galicia (regional government) and by the Campus of International Excellence (CEI)/Campus do Mar. This paper is part of the following research projects carried out by the Estación de Bioloxía Mariña da Graña (USC): PGIDT01PXI20008PR, PGIDIT05 - PXIC20001P, PGIDIT07PXB000120PR, A SELVA-08 and ForSaGal-09 supported by the Dirección Xeral de I + D + i, Xunta de Galicia; VEM2003-20070-C04-04, CGL 2004-22429-E and CTM2004-00740 supported by the Ministry of Education and Science (MEC) of the government of Spain.

References

  1. Alba-Tercedor J, Sánchez-Tocino L (2011) The use of the SkyScan 1172 high resolution micro-CT to elucidate if the spicules of the sea slugs (Mollusca: Nudibranchia, Opisthobranchia) have a structural or a defensive function. SkyScan Users Meet 2011:113–121Google Scholar
  2. Candás M, Díaz-Agras G, Urgorri V (2014) The use of micro-CT for the study of the internal anatomy of sea slugs (Opisthobranchia, Nudibranchia, Dotidae). SkyScan Users Meet 2014:241–243Google Scholar
  3. Candás M, Díaz-Agras G, Abad M, Barrio L, Cunha-Veira X, Pedrouzo L, Pérez-Señarís M, Tato R, García-Álvarez O, Urgorri V (2016) Application of micro-CT in the study of the anatomy of small marine molluscs. Microsc Anal 30(2):S8–S11Google Scholar
  4. Faulwetter S, Dailianis T, Vasileiadou A, Arvanitidis C (2013) Contrast enhancing techniques for the application of micro-CT in marine biodiversity studies. Microsc Anal 27(2):S4–S7Google Scholar
  5. García-Álvarez O, Salvini-Plawen L (2007) Species and diagnosis of the families and genera of Solenogastres (Mollusca). Iberus 25(2):73–143Google Scholar
  6. García-Álvarez O, Urgorri V (2001) Luitfriedia minuta gen. Et sp. nov. (Mollusca: Solenogastres), a new species from Galicia, North-West Spain. Cah Biol Mar 42:197–202Google Scholar
  7. García-Álvarez O, Salvini-Plawen L, Urgorri V (2001) Unciherpia hirsuta a new genus and species of Aplacophoran (Mollusca Solenogastres: Pararrhopaliidae) from Galicia, Northwest Spain. J Molluscan Stud 67:113–119CrossRefGoogle Scholar
  8. García-Álvarez O, Salvini-Plawen Lv, Urgorri V (2014) Solenogastres. In: Mollusca, Solenogastres, Caudofoveata, Monoplacophora. García-Álvarez, O., Salvini-Plawen, L.v., Urgorri, V. & Troncoso, J. S. In: Ramos, M. A. et al. (Eds.). Fauna Ibérica, vol. 38. Museo Nacional de Ciencias Naturales. CSIC. Madrid, pp 31–163Google Scholar
  9. Golding RE, Jones AS (2007) Micro-CT as a novel technique for 3D reconstruction of molluscan anatomy. Molluscan Res 27(3):123–128Google Scholar
  10. Handl C, Todt C (2005) Foregut glands of Solenogastres (Mollusca): anatomy and revised terminology. J Morphol 265:28–42CrossRefGoogle Scholar
  11. Pedrouzo L, Cobo MC, García-Álvarez O, Rueda JL, Gofas S, Urgorri V (2014) Solenogastres (Mollusca) from expeditions off the south Iberian Peninsula, with the description of a new species. J Nat Hist 48(45–48):2985–3006CrossRefGoogle Scholar
  12. Zamarro M, García-Alvarez O, Urgorri V (2013) Three new species of Pruvotinidae (Mollusca: Solenogastres from Antarctica and NW Spain). Helgol Mar Res 67:423–443CrossRefGoogle Scholar
  13. Zamarro M, García-Alvarez O, Urgorri V (2015) New anatomical and biogeographical data on Solenogastres Cavibelonia from the Galician continental margin (NW Spain). Iberus 33(1):1–26Google Scholar

Copyright information

© Senckenberg Gesellschaft für Naturforschung and Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Departamento de Zooloxía e Antropoloxía FísicaUniversidade de Santiago de CompostelaSantiago de CompostelaSpain
  2. 2.Estación de Bioloxía Mariña da GrañaUniversidade de Santiago de CompostelaFerrolSpain

Personalised recommendations