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Marine Biodiversity

, Volume 49, Issue 1, pp 505–508 | Cite as

Characterization of 12 polymorphic microsatellite loci in the Port Jackson Shark, Heterodontus portusjacksoni (Meyer, 1793)

  • Jennalee A. ClarkEmail author
  • Culum Brown
  • Michael R. Gillings
  • Michael Gardner
  • Jane E. Williamson
  • Christopher Izzo
  • Joanna Day
Short Communication

Abstract

The Port Jackson Shark (Heterodontus portusjacksoni) is an epibenthic, oviparous species endemic to the southern half of Australia. Understanding the genetic structure, ecology and reproductive behaviours of this species is essential for developing appropriate management and conservation strategies. However, research has been hampered due a lack of suitable genetic markers. Here, we developed 12 microsatellite loci for H. portusjacksoni. The number of alleles at these polymorphic loci ranged from 3 to 22 and the observed heterozygosity ranged from 0.15 to 0.84. Six of twelve loci successfully cross-amplified in the closely related Crested Hornshark, H. galeatus (Günther, 1870). These markers will aid in studying the spatial and reproductive ecology of H. portusjacksoni and other heterodontid sharks.

Keywords

Elasmobranch Cross-amplification Population structure Dispersal Genotype reconstruction Nuclear DNA 

Notes

Acknowledgments

The authors wish to thank the funding providers Taronga Zoo, the Australian Research Council and Macquarie University; Pascal Geraghty and Liette Vandine for assistance with microsatellite characterization; the researchers and volunteers for assistance with sample collection and Alison Fitch from Flinders University for initial genomic sequencing.

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Copyright information

© Senckenberg Gesellschaft für Naturforschung and Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Jennalee A. Clark
    • 1
    Email author
  • Culum Brown
    • 1
  • Michael R. Gillings
    • 1
  • Michael Gardner
    • 2
    • 3
  • Jane E. Williamson
    • 1
  • Christopher Izzo
    • 4
  • Joanna Day
    • 5
  1. 1.Department of Biological SciencesMacquarie UniversityNorth RydeAustralia
  2. 2.College of Science and EngineeringFlinders UniversityAdelaideAustralia
  3. 3.Evolutionary Biology Unit SA MuseumAdelaideAustralia
  4. 4.School of Biological SciencesThe University of AdelaideAdelaideAustralia
  5. 5.Taronga Conservation Society AustraliaMosmanAustralia

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