Skip to main content
SpringerLink
Log in

Evaluation of oxygen consumption in human vitrified and warmed pre-antral follicles after prolonged low temperatures

  • Original Article
  • Published:
Reproductive Medicine and Biology

Abstract

Purpose

The purpose of this study was to evaluate the effect of transportation at prolonged low temperatures on the survival of pre-antral follicles.

Methods

Ovarian tissue was removed from six women with gender identity disorder. Tissues were stored in an icebox at 4 °C for 6 or 18 h prior to vitrification. After warming, ovarian tissues were cultured for 24 h and follicle survival was assessed via a viability/cytotoxicity kit. Morphological features and oxygen consumption rate (OCR) were evaluated by scanning electrochemical microscopy (SECM).

Results

Survival rate of isolated primordial follicles was 95.7 and 100 %, and that of primary follicles was 91.7 and 81.8 % in the 6- and 18-h groups respectively. There was no difference in morphology between the 6- and 18-h storage groups. In comparison with OCR of vitrified-warmed follicles and OCR of 24-h culture after vitrified-warmed follicles, OCR of 24-h culture after vitrified-warmed primordial follicles was significantly higher in both 6-hour (0.02 ± 0.02 vs 0.07 ± 0.04, P < 0.05) and 18-h groups (0.02 ± 0.02 vs 0.11 ± 0.10, P < 0.05).

Conclusions

This strongly suggests that prolonged transportation of ovarian tissue at low temperatures is useful when there are no available local systems for fertility preservation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Jemal A, Clegg LX, Ward E, Ries LA, Wu X, Jamison PM, et al. Annual report to the nation on the status of cancer, 1975–2001, with a special feature regarding survival. Cancer. 2004;101:3–27.

    Article  PubMed  Google Scholar 

  2. Donnez J, Dolmans MM, Demylle D, Jadoul P, Pirard C, Squifflet J, et al. Livebirth after orthotopic transplantation of cryopreserved ovarian tissue. Lancet. 2004;364:1405–10.

    Article  CAS  PubMed  Google Scholar 

  3. Jeruss JS, Woodruff TK. Preservation of fertility in patients with cancer. N Engl J Med. 2009;360:902–11.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Kolp LA, Hubayter Z. Autotransplantation of cryopreserved ovarian tissue: a procedure with promise, risks, and a need for a registry. Fertil Steril. 2011;95:1879–86.

    Article  PubMed  Google Scholar 

  5. Donnez J, Dolmans MM. Preservation of fertility in females with haematological malignancy. Br J Haematol. 2011;154:75–184.

    Article  Google Scholar 

  6. Donnez J, Jadoul P, Pirard C, Hutchings G, Demylle D, Squifflet J, Smitz J, Dolmans MM. Live birth after transplantation of frozen-thawed ovarian tissue after bilateral oophorectomy for benign disease. Fertil Steril. 2012;98:720–5.

    Article  PubMed  Google Scholar 

  7. Revel A, Laufer N, Ben Meir A, Lebovich M, Mitran E. Micro-organ ovarian transplantation enables pregnancy: a case report. Hum Reprod. 2011;26:1097–103.

    Article  PubMed  Google Scholar 

  8. Silbers S, Kagawa N, Kuwayama M, Gosden R. Duration of fertility after fresh and frozen ovary transplantation. Fertil Steril. 2010;94:2191–6.

    Article  Google Scholar 

  9. Ferguson EM, Leese HJ. A potential role for triglyceride as an energy source during bovine oocyte maturation and early embryo development. Mol Reprod Dev. 2006;73:1195–201.

    Article  CAS  PubMed  Google Scholar 

  10. Magnusson C, Hillensjö T, Hamberger L, Nilsson L. Oxygen consumption by human oocytes and blastocysts grown in vitro. Hum Reprod. 1986;1:183–4.

    CAS  PubMed  Google Scholar 

  11. Preis KA, Seidel GE Jr, Gardner DK. Reduced oxygen concentration improves the developmental competence of mouse oocytes following in vitro maturation. Mol Reprod Dev. 2007;74:893–903.

    Article  CAS  PubMed  Google Scholar 

  12. Tejera A, Herrero J, de Los Santos MJ, Garrido N, Ramsing N, Meseguer M. Oxygen consumption is a quality marker for human oocyte competence conditioned by ovarian stimulation regimens. Fertil Steril. 2011;96:618–23.

    Article  PubMed  Google Scholar 

  13. Kagawa N, Silber S, Kuwayama M. Successful vitrification of bovine and human ovarian tissue. RBM Online. 2009;18:568–77.

    PubMed  Google Scholar 

  14. Shiku H, Shiraishi T, Aoyagi S, Utsumi Y, Matsudaira M, Abe H, et al. Respiration activity of single bovine embryos entrapped in a cone-shaped microwell monitored by scanning electrochemical microscopy. Anal Chim Acta. 2004;522:51–8.

    Article  CAS  Google Scholar 

  15. Van den Hurk R, Spek ER, Hage WJ, Fair T, Ralph JH, Schotanus K. Ultrastructure and viability of isolated bovine preantral follicles. Hum Reprod Update. 1998;4:833–41.

    Article  PubMed  Google Scholar 

  16. Benos DJ, Baladan RS. Energy metabolism of preimplantation mammalian blastocysts. Am J Physiol. 1983;245:40–5.

    Google Scholar 

  17. Leese HJ. What does an embryo need? Hum Fertil. 2003;6:180–5.

    Article  Google Scholar 

  18. Abe H, Shiku H, Yokoo M, et al. Evaluating the quality of individual embryos with a non-invasive and highly sensitive measurement of oxygen consumption by scanning electrochemical microscopy. J Reprod Dev. 2006;52(Suppl):55–64.

    Google Scholar 

  19. Lopes AS, Madsen SE, Ramsing NB, Løvendahl P, Greve T, Callesen H. Investigation of respiration of individual bovine embryos produced in vivo and in vitro and correlation with viability following transfer. Hum Reprod. 2007;22:558–66.

    Article  CAS  PubMed  Google Scholar 

  20. Sakagami N, Yamamoto T, Akiyama K, Nakazawa Y, Kojima N, Nishida K, Yokomizo S, Takagi Y, Abe H, Suzuki C, et al. Viability of porcine embryos after vitrification using water-soluble pullulan films. J Reprod Dev. 2010;56:279–84.

    Article  PubMed  Google Scholar 

  21. Yamanaka M, Hashimoto S, Amo A, Ito-Sasaki T, Abe H, Morimoto Y. Developmental assessment of human vitrified-warmed blastocysts based on oxygen consumption. Hum Reprod. 2011;26:3366–71.

    Article  CAS  PubMed  Google Scholar 

  22. Meirow D, Levron J, Eldar-Geva T, Hardan I, Fridman E, Zalel Y, Schiff E, Dor J. Pregnancy after transplantation of cryopreserved ovarian tissue in a patient with ovarian failure after chemotherapy. N Engl J Med. 2005;353:318–21.

    Article  CAS  PubMed  Google Scholar 

  23. Andersen CY, Rosendahl M, Byskov AG, Loft A, Ottosen C, Dueholm M, Schmidt KL, Andersen AN, Ernst E. Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue. Hum Reprod. 2008;23:2266–72.

    Article  PubMed  Google Scholar 

  24. Silber SJ, DeRosa M, Pineda J, et al. A series of monozygotic twins discordant for ovarian failure: ovary transplantation (cortical versus microvascular) and cryopreservation. Hum Reprod. 2008;23:1531–7.

    Article  CAS  PubMed  Google Scholar 

  25. Kuleshova LL, Lopata A. Vitrification can be more favourable than slow cooling. Fertil Steril. 2002;78:449–54.

    Article  PubMed  Google Scholar 

  26. Courbiere B, Odagescu V, Baudot A, Massardier J, Mazoyer C, Salle B, Lornage J. Cryopreservation of the ovary by vitrification as an alternative to slow-cooling protocols. Fertil Steril. 2006;86((Suppl 3)):1243–51.

    Article  PubMed  Google Scholar 

  27. Schmidt KL, Ernst E, Byskov AG, Andersen AN, Andersen CY. Survival of primordial follicles following prolonged transportation of ovarian tissue prior to cryopreservation. Hum Reprod. 2003;18:2654–9.

    Article  CAS  PubMed  Google Scholar 

  28. Schmidt KT, Rosendahl M, Ernst E, Loft A, Andersen AN, Dueholm M, Ottosen C, Andersen CY. Autotransplantation of cryopreserved ovarian tissue in 12 women with chemotherapy-induced premature ovarian failure: the Danish experience. Fertil Steril. 2011;95:695–701.

    Article  PubMed  Google Scholar 

  29. Dittrich R, Lotz L, Kech G, Hoffmann I, Mueller A, Bechmann MW, Van der Ven H, Montag M. Live birth after ovarian tissue autotransplantation following overnight transportation before cryopreservation. Fertil Steril. 2012;97:387–90.

    Article  PubMed  Google Scholar 

  30. Rosendahl M, Schmidt KT, Ernst E, Rasmussen PE, Loft A, Byskov AG, et al. Cryopreservation of ovarian tissue for a decade in Denmark: a view of the technique. RBM Online. 2011;22:162–71.

    PubMed  Google Scholar 

  31. Isachenko E, Isachenko V, Nawroth F, Rahimi G, Weiss JM. Effect of long-term exposure at suprazero temperatures on activity and viability of human ovarian cortex. Fertil Steril. 2009;91:1556–9.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Kyono ART Clinic, Mitsuiseimei, Sendai Honcho Bldg, 3F, 1-1-1 Honcho, Aoba-ku, Sendai, Miyagi, 980-0014, Japan

    Toshihiko Kyoya, Yusuke Nakamura, Shizue Miyatani, Tomoko Miyagawa & Koichi Kyono

  2. Osaka New ART Clinic, Osaka, Japan

    Tatsuhiro Tomiyama

Authors
  1. Toshihiko Kyoya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yusuke Nakamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shizue Miyatani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tomoko Miyagawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tatsuhiro Tomiyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Koichi Kyono
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Koichi Kyono.

About this article

Cite this article

Kyoya, T., Nakamura, Y., Miyatani, S. et al. Evaluation of oxygen consumption in human vitrified and warmed pre-antral follicles after prolonged low temperatures. Reprod Med Biol 13, 47–52 (2014). https://doi.org/10.1007/s12522-013-0166-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12522-013-0166-9

Keywords

Search

Navigation