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Archaeological and Anthropological Sciences

, Volume 11, Issue 7, pp 3459–3475 | Cite as

Ancient mitochondrial DNA and population dynamics in postclassic Central Mexico: Tlatelolco (ad 1325–1520) and Cholula (ad 900–1350)

  • Ana Y. Morales-ArceEmail author
  • Geoffrey McCafferty
  • Jessica Hand
  • Norma Schmill
  • Krista McGrath
  • Camilla SpellerEmail author
Original Paper

Abstract

The past composition and genetic diversity of populations from Central Mexico during the Postclassic period (ad 900–1520) are still little understood. Two of the largest centres of ancient groups, Tlatelolco and Cholula, declined after European conquest and questions about their relationships with other Central Mexican cities and ritual activities have been debated. Tlatelolco was a Mexica group that practiced the Quetzalcoatl cult and human sacrifice, including the sacrifice of children, while Cholula was considered the main pilgrimage centre and multiethnic city during the Postclassic. This study analysed the mitochondrial DNA control region of 28 human skeletal samples to estimate the genetic affinities of individuals buried at Tlatelolco and Cholula. Amelogenin analysis and whole genome sequencing (WGS) were also applied to determine the sex of the 15 Tlatelolco subadults from sacrificial contexts. Networks, PCoA and Nei genetic distances were calculated to compare Tlatelolco and Cholula haplotypes with available ancient haplotype data from Mesoamerican groups and the two borderland areas, Paquimé and Greater Nicoya. Mitochondrial haplogroups were characterized for 11 of the 15 samples from Tlatelolco (73%) and 12 samples out of 13 from Cholula (92%), revealing the presence of four distinct Amerindian mitochondrial lineages at Tlateloloco, A (n = 6; 55%), B (n = 2; 18%), C (n = 1; 9%) and D (n = 2; 10%); and three lineages in Cholula, A (n = 5; 42%), B (n = 5; 42%) and C (n = 2; 16%). Statistical analysis of the haplotypes, haplogroup frequencies and Nei genetic distances showed close affinity of Tlatelolco’s subadults with ancient Mexica (Aztecs) and closer affinities between Cholula and the Xaltocan of the Basin of Mexico. Sex determination of Tlatelolco subadult sacrifice victims revealed that 83% were females, in contrast to previous studies of subadult sacrificial patterns at the site. Together, these results demonstrate the multi-ethnic nature of religious and economic centres in Postclassic Central Mexico.

Keywords

Mesoamerica Human sacrifice Mitochondrial DNA Genetic distance Haplogroups Sex determination 

Notes

Acknowledgements

The authors thank Dirección de Antropología Física, INAH; INAH-Tlatelolco and INAH-Puebla for providing information and permissions to conduct this study. We give special thanks to MSc. Jose Antonio Pompa y Padilla and MSc. Ma. Elena Salas Cuesta† who selected the Tlatelolco samples and sent them for genetic analysis to the University of Calgary. Also, we thank the archaeologists Carlos Cedillo Ortega and Martha Adriana Saenz Serdio, for providing information and selecting samples from Cholula for this research. Likewise, we thank Salvador Guilliem Arroyo, who provided information about the samples obtained from Tlatelolco. Finally, we thank to Adam K. Benfer for making the map for this article.

Author contributions were as follows: AYMA, NS and CS designed the research. AYMA, JH and KM performed the experiments. AYMA, JH and CS analysed the data. GM, CS and NS provided materials and resources. AYMA wrote the paper, with input from the other co-authors.

Funding

This work was supported by the University Research Grants seed grant from the University of Calgary, and by BioArch, University of York.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

12520_2018_771_MOESM1_ESM.xlsx (13 kb)
ESM 1 Results of whole genome sequencing of Tlatelolco subadults. (XLSX 13 kb)
12520_2018_771_MOESM2_ESM.docx (762 kb)
ESM 2 (DOCX 762 kb)

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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Center for Evolution and MedicineArizona State UniversityTempeUSA
  2. 2.Department of Anthropology and ArchaeologyUniversity of CalgaryCalgaryCanada
  3. 3.BioArCh, Department of ArchaeologyUniversity of YorkYorkUK
  4. 4.Gen Assoc AHS Researcher, Cumming School of MedicineUniversity of CalgaryCalgaryCanada
  5. 5.Department of AnthropologyUniversity of British ColumbiaVancouverCanada

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